Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration
A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric t...
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Veröffentlicht in: | Analytical biochemistry 2000-08, Vol.283 (2), p.166-174 |
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creator | He, Z.K. Fuhrmann, B. Spohn, U. |
description | A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric titration of ammonia with hypobromite. The hypobromite is formed after the electrogeneration of bromine in an electrolyte containing 1.0 M NaBr and 0.1 M sodium borate adjusted to pH 8.5. The electrolysis current follows a triangle-programmed current-time course. An amperometric flow detector records the resulting mirror symmetrical titration curves, which show two equivalence points. The analyte concentration is calculated from the time difference between the equivalence points. For quantitative conversion of creatinine and quantitative separation of present and released ammonia no calibration is necessary to get accurate results. Both ammonia/ammonium and creatinine were determined in the range between 2 μM and 2 mM with relative standard deviations between 3.0 and 1.0% (n = 5). High recoveries were obtained for the analysis of diluted urine samples for both creatinine and ammonia. |
doi_str_mv | 10.1006/abio.2000.4627 |
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This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric titration of ammonia with hypobromite. The hypobromite is formed after the electrogeneration of bromine in an electrolyte containing 1.0 M NaBr and 0.1 M sodium borate adjusted to pH 8.5. The electrolysis current follows a triangle-programmed current-time course. An amperometric flow detector records the resulting mirror symmetrical titration curves, which show two equivalence points. The analyte concentration is calculated from the time difference between the equivalence points. For quantitative conversion of creatinine and quantitative separation of present and released ammonia no calibration is necessary to get accurate results. Both ammonia/ammonium and creatinine were determined in the range between 2 μM and 2 mM with relative standard deviations between 3.0 and 1.0% (n = 5). High recoveries were obtained for the analysis of diluted urine samples for both creatinine and ammonia.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.2000.4627</identifier><identifier>PMID: 10906237</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aminohydrolases - chemistry ; ammonia ; Ammonia - analysis ; Calibration ; calibrationless determinations ; Chemistry Techniques, Analytical - methods ; coulometric flow titration ; creatinine ; Creatinine - analysis ; Creatinine - urine ; creatinine iminohydrolase ; Electrochemistry ; Humans ; Quality Control ; Titrimetry - methods</subject><ispartof>Analytical biochemistry, 2000-08, Vol.283 (2), p.166-174</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-899e374a988004996dc2a47bec17ad5fc768bbf369edb084bf70da2b1885feae3</citedby><cites>FETCH-LOGICAL-c340t-899e374a988004996dc2a47bec17ad5fc768bbf369edb084bf70da2b1885feae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abio.2000.4627$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10906237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Z.K.</creatorcontrib><creatorcontrib>Fuhrmann, B.</creatorcontrib><creatorcontrib>Spohn, U.</creatorcontrib><title>Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric titration of ammonia with hypobromite. The hypobromite is formed after the electrogeneration of bromine in an electrolyte containing 1.0 M NaBr and 0.1 M sodium borate adjusted to pH 8.5. The electrolysis current follows a triangle-programmed current-time course. An amperometric flow detector records the resulting mirror symmetrical titration curves, which show two equivalence points. The analyte concentration is calculated from the time difference between the equivalence points. For quantitative conversion of creatinine and quantitative separation of present and released ammonia no calibration is necessary to get accurate results. Both ammonia/ammonium and creatinine were determined in the range between 2 μM and 2 mM with relative standard deviations between 3.0 and 1.0% (n = 5). High recoveries were obtained for the analysis of diluted urine samples for both creatinine and ammonia.</description><subject>Aminohydrolases - chemistry</subject><subject>ammonia</subject><subject>Ammonia - analysis</subject><subject>Calibration</subject><subject>calibrationless determinations</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>coulometric flow titration</subject><subject>creatinine</subject><subject>Creatinine - analysis</subject><subject>Creatinine - urine</subject><subject>creatinine iminohydrolase</subject><subject>Electrochemistry</subject><subject>Humans</subject><subject>Quality Control</subject><subject>Titrimetry - methods</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFPwzAQRi0EoqWwMiJPbAnnJLXjsQoUkCqxtLNlOxfJKImLnYL670kJAwvTnT69-6R7hNwySBkAf9DG-TQDgLTgmTgjcwaSJ5CDPCfzMc6TjEsxI1cxvgMwViz5JZmNEPAsF3Oyq3TrTNCD832LMdJHHDB0rv9JqG9oFXDce9cj1X1NV13ne6epOdLKH1rf4RCcpevWf9GtG6ama3LR6Dbize9ckN36aVu9JJu359dqtUlsXsCQlFJiLgotyxKgkJLXNtOFMGiZ0PWysYKXxjQ5l1gbKAvTCKh1ZlhZLhvUmC_I_dS7D_7jgHFQnYsW21b36A9RCZYVnEk-gukE2uBjDNiofXCdDkfFQJ1EqpNIdRKpTiLHg7vf5oPpsP6DT-ZGoJwAHP_7dBhUtA57i7ULaAdVe_df9zdOdoMc</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>He, Z.K.</creator><creator>Fuhrmann, B.</creator><creator>Spohn, U.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration</title><author>He, Z.K. ; Fuhrmann, B. ; Spohn, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-899e374a988004996dc2a47bec17ad5fc768bbf369edb084bf70da2b1885feae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aminohydrolases - chemistry</topic><topic>ammonia</topic><topic>Ammonia - analysis</topic><topic>Calibration</topic><topic>calibrationless determinations</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>coulometric flow titration</topic><topic>creatinine</topic><topic>Creatinine - analysis</topic><topic>Creatinine - urine</topic><topic>creatinine iminohydrolase</topic><topic>Electrochemistry</topic><topic>Humans</topic><topic>Quality Control</topic><topic>Titrimetry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Z.K.</creatorcontrib><creatorcontrib>Fuhrmann, B.</creatorcontrib><creatorcontrib>Spohn, U.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Z.K.</au><au>Fuhrmann, B.</au><au>Spohn, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>283</volume><issue>2</issue><spage>166</spage><epage>174</epage><pages>166-174</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream, and coulometric titration of ammonia with hypobromite. The hypobromite is formed after the electrogeneration of bromine in an electrolyte containing 1.0 M NaBr and 0.1 M sodium borate adjusted to pH 8.5. The electrolysis current follows a triangle-programmed current-time course. An amperometric flow detector records the resulting mirror symmetrical titration curves, which show two equivalence points. The analyte concentration is calculated from the time difference between the equivalence points. For quantitative conversion of creatinine and quantitative separation of present and released ammonia no calibration is necessary to get accurate results. Both ammonia/ammonium and creatinine were determined in the range between 2 μM and 2 mM with relative standard deviations between 3.0 and 1.0% (n = 5). High recoveries were obtained for the analysis of diluted urine samples for both creatinine and ammonia.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10906237</pmid><doi>10.1006/abio.2000.4627</doi><tpages>9</tpages></addata></record> |
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subjects | Aminohydrolases - chemistry ammonia Ammonia - analysis Calibration calibrationless determinations Chemistry Techniques, Analytical - methods coulometric flow titration creatinine Creatinine - analysis Creatinine - urine creatinine iminohydrolase Electrochemistry Humans Quality Control Titrimetry - methods |
title | Calibrationless Determination of Creatinine and Ammonia by Coulometric Flow Titration |
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