Catalytic Roles for Two Water Bridged Residues (Asp-98 and His-255) in the Active Site of Copper-containing Nitrite Reductase

Two active site residues, Asp-98 and His-255, of copper-containing nitrite reductase (NIR) from Alcaligenes faecalis have been mutated to probe the catalytic mechanism. Three mutations at these two sites (D98N, H255D, and H255N) result in large reductions in activity relative to native NIR, suggesti...

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Veröffentlicht in:	The Journal of biological chemistry 2000-08, Vol.275 (31), p.23957-23964
Hauptverfasser:	Boulanger, Martin J., Kukimoto, Mutsuko, Nishiyama, Makoto, Horinouchi, Sueharu, Murphy, Michael E.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcaligenes - enzymology Aspartic Acid Catalysis Catalytic Domain - genetics Copper Crystallography, X-Ray Electron Spin Resonance Spectroscopy Histidine Hydrogen Bonding Models, Molecular Mutation Nitrite Reductases - chemistry Nitrite Reductases - genetics Water
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