Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin
The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH(4)Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore rad...
Gespeichert in:
| Veröffentlicht in: | American journal of physiology. Renal physiology 2000-07, Vol.279 (1), p.F84-F91 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | F91 |
|---|---|
| container_issue | 1 |
| container_start_page | F84 |
| container_title | American journal of physiology. Renal physiology |
| container_volume | 279 |
| creator | Ohlson, M Sörensson, J Haraldsson, B |
| description | The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH(4)Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore radii of 46 A and 80-87 A, respectively. The estimated negative charge density was 35-45 meq/l at 8 degrees C. Perfusion with erythrocyte-free solutions of kidneys at 37 degrees C reduced glomerular size and charge permselectivity. Thus the large pore fraction of the glomerular filtrate (f(L)) was 1.64% at 37 degrees C compared with 0.94% at 8 degrees C. The theta for albumin was four times higher at 37 degrees C than at 8 degrees C (0.86% vs. 0.19%, respectively). NH(4)Cl caused further irreversible damage to the glomerular barrier. We conclude that there are no deleterious effects on the glomerular barrier of a reduction in temperature from 37 degrees C to 8 degrees C. Therefore our data seem to disprove the hypothesis of low glomerular permselectivity and transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier. |
| doi_str_mv | 10.1152/ajprenal.2000.279.1.f84 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71240241</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71240241</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-31ff6eab9619cd11c4c343dfcf4f2038e73ed8c2926452ac6c91538d526a42b63</originalsourceid><addsrcrecordid>eNpNkE1LxDAQhoMofv8FzclbayZJ0_Yoi7suLHhR2FtI04lW0nZN2oX119t1FTzNC_O8M_AQcgssBcj4vfnYBOyMTzljLOV5mULqCnlEzqctT0AqdTzlUkBSZPn6jFzE-DGhABxOyRmwopR5yc7JeuH7FsPoTaCx-UJqupradxPekEb0aIdm2ww72nR0eEcazEBNpAG3aDzWtNrR-fJllrjG9t7_lI2vxrbprsiJMz7i9e-8JK_zx5fZU7J6XixnD6vESiWGRIBzCk1VKihtDWClFVLUzjrpOBMF5gLrwvKSK5lxY5UtIRNFnXFlJK-UuCR3h7ub0H-OGAfdNtGi96bDfow6By4ZlzCB-QG0oY8xoNOb0LQm7DQwvZeq_6TqvVQ9SdWg54Wcmje_L8aqxfpf72BRfAMobHW3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71240241</pqid></control><display><type>article</type><title>Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin</title><source>MEDLINE</source><source>American Physiological Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Ohlson, M ; Sörensson, J ; Haraldsson, B</creator><creatorcontrib>Ohlson, M ; Sörensson, J ; Haraldsson, B</creatorcontrib><description>The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH(4)Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore radii of 46 A and 80-87 A, respectively. The estimated negative charge density was 35-45 meq/l at 8 degrees C. Perfusion with erythrocyte-free solutions of kidneys at 37 degrees C reduced glomerular size and charge permselectivity. Thus the large pore fraction of the glomerular filtrate (f(L)) was 1.64% at 37 degrees C compared with 0.94% at 8 degrees C. The theta for albumin was four times higher at 37 degrees C than at 8 degrees C (0.86% vs. 0.19%, respectively). NH(4)Cl caused further irreversible damage to the glomerular barrier. We conclude that there are no deleterious effects on the glomerular barrier of a reduction in temperature from 37 degrees C to 8 degrees C. Therefore our data seem to disprove the hypothesis of low glomerular permselectivity and transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier.</description><identifier>ISSN: 1931-857X</identifier><identifier>EISSN: 1522-1466</identifier><identifier>DOI: 10.1152/ajprenal.2000.279.1.f84</identifier><identifier>PMID: 10894790</identifier><language>eng</language><publisher>United States</publisher><subject>Ammonium Chloride - pharmacology ; Animals ; Biological Transport - drug effects ; Calibration ; Diffusion ; Ficoll - analogs & derivatives ; Ficoll - chemistry ; Ficoll - metabolism ; Fluorescein-5-isothiocyanate - analogs & derivatives ; Fluorescein-5-isothiocyanate - chemistry ; Fluorescein-5-isothiocyanate - metabolism ; Glomerular Filtration Rate ; In Vitro Techniques ; Kidney Glomerulus - blood supply ; Kidney Glomerulus - drug effects ; Kidney Glomerulus - metabolism ; Kidney Glomerulus - physiology ; Male ; Models, Biological ; Perfusion ; Rats ; Rats, Wistar ; Serum Albumin - chemistry ; Serum Albumin - metabolism ; Substrate Specificity ; Temperature ; Time Factors</subject><ispartof>American journal of physiology. Renal physiology, 2000-07, Vol.279 (1), p.F84-F91</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-31ff6eab9619cd11c4c343dfcf4f2038e73ed8c2926452ac6c91538d526a42b63</citedby><cites>FETCH-LOGICAL-c463t-31ff6eab9619cd11c4c343dfcf4f2038e73ed8c2926452ac6c91538d526a42b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10894790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohlson, M</creatorcontrib><creatorcontrib>Sörensson, J</creatorcontrib><creatorcontrib>Haraldsson, B</creatorcontrib><title>Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin</title><title>American journal of physiology. Renal physiology</title><addtitle>Am J Physiol Renal Physiol</addtitle><description>The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH(4)Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore radii of 46 A and 80-87 A, respectively. The estimated negative charge density was 35-45 meq/l at 8 degrees C. Perfusion with erythrocyte-free solutions of kidneys at 37 degrees C reduced glomerular size and charge permselectivity. Thus the large pore fraction of the glomerular filtrate (f(L)) was 1.64% at 37 degrees C compared with 0.94% at 8 degrees C. The theta for albumin was four times higher at 37 degrees C than at 8 degrees C (0.86% vs. 0.19%, respectively). NH(4)Cl caused further irreversible damage to the glomerular barrier. We conclude that there are no deleterious effects on the glomerular barrier of a reduction in temperature from 37 degrees C to 8 degrees C. Therefore our data seem to disprove the hypothesis of low glomerular permselectivity and transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier.</description><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Calibration</subject><subject>Diffusion</subject><subject>Ficoll - analogs & derivatives</subject><subject>Ficoll - chemistry</subject><subject>Ficoll - metabolism</subject><subject>Fluorescein-5-isothiocyanate - analogs & derivatives</subject><subject>Fluorescein-5-isothiocyanate - chemistry</subject><subject>Fluorescein-5-isothiocyanate - metabolism</subject><subject>Glomerular Filtration Rate</subject><subject>In Vitro Techniques</subject><subject>Kidney Glomerulus - blood supply</subject><subject>Kidney Glomerulus - drug effects</subject><subject>Kidney Glomerulus - metabolism</subject><subject>Kidney Glomerulus - physiology</subject><subject>Male</subject><subject>Models, Biological</subject><subject>Perfusion</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - metabolism</subject><subject>Substrate Specificity</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>1931-857X</issn><issn>1522-1466</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LxDAQhoMofv8FzclbayZJ0_Yoi7suLHhR2FtI04lW0nZN2oX119t1FTzNC_O8M_AQcgssBcj4vfnYBOyMTzljLOV5mULqCnlEzqctT0AqdTzlUkBSZPn6jFzE-DGhABxOyRmwopR5yc7JeuH7FsPoTaCx-UJqupradxPekEb0aIdm2ww72nR0eEcazEBNpAG3aDzWtNrR-fJllrjG9t7_lI2vxrbprsiJMz7i9e-8JK_zx5fZU7J6XixnD6vESiWGRIBzCk1VKihtDWClFVLUzjrpOBMF5gLrwvKSK5lxY5UtIRNFnXFlJK-UuCR3h7ub0H-OGAfdNtGi96bDfow6By4ZlzCB-QG0oY8xoNOb0LQm7DQwvZeq_6TqvVQ9SdWg54Wcmje_L8aqxfpf72BRfAMobHW3</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Ohlson, M</creator><creator>Sörensson, J</creator><creator>Haraldsson, B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin</title><author>Ohlson, M ; Sörensson, J ; Haraldsson, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-31ff6eab9619cd11c4c343dfcf4f2038e73ed8c2926452ac6c91538d526a42b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Ammonium Chloride - pharmacology</topic><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Calibration</topic><topic>Diffusion</topic><topic>Ficoll - analogs & derivatives</topic><topic>Ficoll - chemistry</topic><topic>Ficoll - metabolism</topic><topic>Fluorescein-5-isothiocyanate - analogs & derivatives</topic><topic>Fluorescein-5-isothiocyanate - chemistry</topic><topic>Fluorescein-5-isothiocyanate - metabolism</topic><topic>Glomerular Filtration Rate</topic><topic>In Vitro Techniques</topic><topic>Kidney Glomerulus - blood supply</topic><topic>Kidney Glomerulus - drug effects</topic><topic>Kidney Glomerulus - metabolism</topic><topic>Kidney Glomerulus - physiology</topic><topic>Male</topic><topic>Models, Biological</topic><topic>Perfusion</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin - metabolism</topic><topic>Substrate Specificity</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohlson, M</creatorcontrib><creatorcontrib>Sörensson, J</creatorcontrib><creatorcontrib>Haraldsson, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Renal physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohlson, M</au><au>Sörensson, J</au><au>Haraldsson, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin</atitle><jtitle>American journal of physiology. Renal physiology</jtitle><addtitle>Am J Physiol Renal Physiol</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>279</volume><issue>1</issue><spage>F84</spage><epage>F91</epage><pages>F84-F91</pages><issn>1931-857X</issn><eissn>1522-1466</eissn><abstract>The fractional clearances (theta) for FITC-Ficoll and albumin were estimated in isolated perfused rat kidneys in which the tubular activity was inhibited by low temperature (8 degrees C) and/or 10 mM NH(4)Cl. The Ficoll data were analyzed according to a two-pore model giving small and large pore radii of 46 A and 80-87 A, respectively. The estimated negative charge density was 35-45 meq/l at 8 degrees C. Perfusion with erythrocyte-free solutions of kidneys at 37 degrees C reduced glomerular size and charge permselectivity. Thus the large pore fraction of the glomerular filtrate (f(L)) was 1.64% at 37 degrees C compared with 0.94% at 8 degrees C. The theta for albumin was four times higher at 37 degrees C than at 8 degrees C (0.86% vs. 0.19%, respectively). NH(4)Cl caused further irreversible damage to the glomerular barrier. We conclude that there are no deleterious effects on the glomerular barrier of a reduction in temperature from 37 degrees C to 8 degrees C. Therefore our data seem to disprove the hypothesis of low glomerular permselectivity and transtubular uptake of intact albumin and support the classic concept of a highly selective glomerular barrier.</abstract><cop>United States</cop><pmid>10894790</pmid><doi>10.1152/ajprenal.2000.279.1.f84</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1931-857X |
| ispartof | American journal of physiology. Renal physiology, 2000-07, Vol.279 (1), p.F84-F91 |
| issn | 1931-857X 1522-1466 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71240241 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Ammonium Chloride - pharmacology Animals Biological Transport - drug effects Calibration Diffusion Ficoll - analogs & derivatives Ficoll - chemistry Ficoll - metabolism Fluorescein-5-isothiocyanate - analogs & derivatives Fluorescein-5-isothiocyanate - chemistry Fluorescein-5-isothiocyanate - metabolism Glomerular Filtration Rate In Vitro Techniques Kidney Glomerulus - blood supply Kidney Glomerulus - drug effects Kidney Glomerulus - metabolism Kidney Glomerulus - physiology Male Models, Biological Perfusion Rats Rats, Wistar Serum Albumin - chemistry Serum Albumin - metabolism Substrate Specificity Temperature Time Factors |
| title | Glomerular size and charge selectivity in the rat as revealed by FITC-ficoll and albumin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T04%3A14%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glomerular%20size%20and%20charge%20selectivity%20in%20the%20rat%20as%20revealed%20by%20FITC-ficoll%20and%20albumin&rft.jtitle=American%20journal%20of%20physiology.%20Renal%20physiology&rft.au=Ohlson,%20M&rft.date=2000-07-01&rft.volume=279&rft.issue=1&rft.spage=F84&rft.epage=F91&rft.pages=F84-F91&rft.issn=1931-857X&rft.eissn=1522-1466&rft_id=info:doi/10.1152/ajprenal.2000.279.1.f84&rft_dat=%3Cproquest_cross%3E71240241%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71240241&rft_id=info:pmid/10894790&rfr_iscdi=true |