Replication of Yeast rDNA Initiates Downstream of Transcriptionally Active Genes
In the yeast S. cerevisiae, ARS (autonomously replicating sequence) elements located in the intergenic spacers of the rRNA gene locus are infrequently activated as origins of replication. We analyzed the rARS activation with a combination of neutral/neutral (N/N) two-dimensional (2D) gel electrophor...
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| Veröffentlicht in: | Molecular cell 2000-05, Vol.5 (5), p.767-777 |
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| creator | Muller, Martine Lucchini, Renzo Sogo, José M |
| description | In the yeast
S. cerevisiae, ARS (autonomously replicating sequence) elements located in the intergenic spacers of the rRNA gene locus are infrequently activated as origins of replication. We analyzed the rARS activation with a combination of neutral/neutral (N/N) two-dimensional (2D) gel electrophoresis and either the intercalating drug psoralen, which in vivo specifically marks the transcribing gene copies, or the selective accessibility of restriction sites in transcriptionally active genes. We found that initiation of replication starts at those rARSs placed immediately downstream of transcribing rRNA genes. This correlation between transcription and replication is consistent with the presence of nucleosome-free enhancers at each transcriptionally active gene copy and suggests that the transcription factor Abf1p is involved in replication initiation at the ARS in the rDNA gene locus. |
| doi_str_mv | 10.1016/S1097-2765(00)80317-2 |
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S. cerevisiae, ARS (autonomously replicating sequence) elements located in the intergenic spacers of the rRNA gene locus are infrequently activated as origins of replication. We analyzed the rARS activation with a combination of neutral/neutral (N/N) two-dimensional (2D) gel electrophoresis and either the intercalating drug psoralen, which in vivo specifically marks the transcribing gene copies, or the selective accessibility of restriction sites in transcriptionally active genes. We found that initiation of replication starts at those rARSs placed immediately downstream of transcribing rRNA genes. This correlation between transcription and replication is consistent with the presence of nucleosome-free enhancers at each transcriptionally active gene copy and suggests that the transcription factor Abf1p is involved in replication initiation at the ARS in the rDNA gene locus.</description><identifier>ISSN: 1097-2765</identifier><identifier>EISSN: 1097-4164</identifier><identifier>DOI: 10.1016/S1097-2765(00)80317-2</identifier><identifier>PMID: 10882113</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Abf1 protein ; DNA Replication ; DNA, Fungal - drug effects ; DNA, Fungal - genetics ; DNA, Ribosomal - drug effects ; DNA, Ribosomal - genetics ; Electrophoresis, Gel, Two-Dimensional ; Ficusin - pharmacology ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Replication Origin - genetics ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Transcription, Genetic</subject><ispartof>Molecular cell, 2000-05, Vol.5 (5), p.767-777</ispartof><rights>2000 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-ff43935aac01cac5aed0d3dedf5aae54a4f074a88eeacdcdaafb6417349064193</citedby><cites>FETCH-LOGICAL-c505t-ff43935aac01cac5aed0d3dedf5aae54a4f074a88eeacdcdaafb6417349064193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1097-2765(00)80317-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10882113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muller, Martine</creatorcontrib><creatorcontrib>Lucchini, Renzo</creatorcontrib><creatorcontrib>Sogo, José M</creatorcontrib><title>Replication of Yeast rDNA Initiates Downstream of Transcriptionally Active Genes</title><title>Molecular cell</title><addtitle>Mol Cell</addtitle><description>In the yeast
S. cerevisiae, ARS (autonomously replicating sequence) elements located in the intergenic spacers of the rRNA gene locus are infrequently activated as origins of replication. We analyzed the rARS activation with a combination of neutral/neutral (N/N) two-dimensional (2D) gel electrophoresis and either the intercalating drug psoralen, which in vivo specifically marks the transcribing gene copies, or the selective accessibility of restriction sites in transcriptionally active genes. We found that initiation of replication starts at those rARSs placed immediately downstream of transcribing rRNA genes. This correlation between transcription and replication is consistent with the presence of nucleosome-free enhancers at each transcriptionally active gene copy and suggests that the transcription factor Abf1p is involved in replication initiation at the ARS in the rDNA gene locus.</description><subject>Abf1 protein</subject><subject>DNA Replication</subject><subject>DNA, Fungal - drug effects</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal - drug effects</subject><subject>DNA, Ribosomal - genetics</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Ficusin - pharmacology</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Replication Origin - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription, Genetic</subject><issn>1097-2765</issn><issn>1097-4164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4MoPqofQdmT6KE6aZJ9nKS0PgqiovXgKUyTWYhsd2uSKn57d9sevPU0D37_GfgxdsrhigNPr984FFl_kKXqAuAyB8HbaYcdrtaSp3J303fIATsK4ROAS5UX--yAQ54POBeH7OWVFpUzGF1TJ02ZfBCGmPjx0zCZ1C46jBSScfNTh-gJ5x0y9VgH492iy2BV_SZDE903JfdUUzhmeyVWgU42tcfe726no4f-4_P9ZDR87BsFKvbLUopCKEQD3KBRSBassGTLdkdKoiwhk5jnRGissYjlLJU8E7KAthaix87Xdxe--VpSiHrugqGqwpqaZdAZHwiZ5vlWkGdKpgI6UK1B45sQPJV64d0c_a_moDvneuVcd0I1gF4514M2d7Z5sJzNyf5LrSW3wM0aoNbHtyOvg3FUG7LOk4naNm7Liz_k2JIc</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Muller, Martine</creator><creator>Lucchini, Renzo</creator><creator>Sogo, José M</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Replication of Yeast rDNA Initiates Downstream of Transcriptionally Active Genes</title><author>Muller, Martine ; Lucchini, Renzo ; Sogo, José M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-ff43935aac01cac5aed0d3dedf5aae54a4f074a88eeacdcdaafb6417349064193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Abf1 protein</topic><topic>DNA Replication</topic><topic>DNA, Fungal - drug effects</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal - drug effects</topic><topic>DNA, Ribosomal - genetics</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Ficusin - pharmacology</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Replication Origin - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muller, Martine</creatorcontrib><creatorcontrib>Lucchini, Renzo</creatorcontrib><creatorcontrib>Sogo, José M</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muller, Martine</au><au>Lucchini, Renzo</au><au>Sogo, José M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Replication of Yeast rDNA Initiates Downstream of Transcriptionally Active Genes</atitle><jtitle>Molecular cell</jtitle><addtitle>Mol Cell</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>5</volume><issue>5</issue><spage>767</spage><epage>777</epage><pages>767-777</pages><issn>1097-2765</issn><eissn>1097-4164</eissn><abstract>In the yeast
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| subjects | Abf1 protein DNA Replication DNA, Fungal - drug effects DNA, Fungal - genetics DNA, Ribosomal - drug effects DNA, Ribosomal - genetics Electrophoresis, Gel, Two-Dimensional Ficusin - pharmacology Gene Expression Regulation, Fungal Genes, Fungal Replication Origin - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transcription, Genetic |
| title | Replication of Yeast rDNA Initiates Downstream of Transcriptionally Active Genes |
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