Lens-Specific VEGF-A Expression Induces Angioblast Migration and Proliferation and Stimulates Angiogenic Remodeling
Vascular endothelial growth factor (VEGF) is a secreted mitogen which specifically stimulates proliferation of vascular endothelial cells in vitro and in vivo. Its expression pattern is consistent with it being an important regulator of vasculogenesis and angiogenesis, and targeted disruption of VEG...
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| Veröffentlicht in: | Developmental biology 2000-07, Vol.223 (2), p.383-398 |
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| container_title | Developmental biology |
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| creator | Ash, John D. Overbeek, Paul A. |
| description | Vascular endothelial growth factor (VEGF) is a secreted mitogen which specifically stimulates proliferation of vascular endothelial cells in vitro and in vivo. Its expression pattern is consistent with it being an important regulator of vasculogenesis and angiogenesis, and targeted disruption of VEGF-A has demonstrated that it is essential for vascular development. To determine if VEGF-A was sufficient to alter vascularization in the eye we generated transgenic mice which express human VEGF-A165 specifically in the lens. Expression of transgenic VEGF-A led to excessive proliferation and accumulation of disorganized angioblasts and endothelial cells around the lens. The results support the hypothesis that VEGF-A can initiate the process of vascularization by stimulating chemoattraction and proliferation of angioblasts and endothelial cells and that VEGF-A expression can stimulate angiogenic remodeling. However, VEGF-A alone was not sufficient to direct blood vessel organization or maturation. |
| doi_str_mv | 10.1006/dbio.2000.9755 |
| format | Article |
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However, VEGF-A alone was not sufficient to direct blood vessel organization or maturation.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.2000.9755</identifier><identifier>PMID: 10882523</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>angioblasts ; angiogenesis ; Animals ; Basement Membrane - metabolism ; Collagen - biosynthesis ; Endothelial Growth Factors - genetics ; Endothelium, Vascular - cytology ; Eye - blood supply ; Eye - embryology ; Eye - growth & development ; eye development ; Humans ; Hyperplasia ; Laminin - biosynthesis ; Lens, Crystalline - blood supply ; Lens, Crystalline - embryology ; Lens, Crystalline - growth & development ; Lymphokines - genetics ; Mesoderm - cytology ; Mice ; Mice, Transgenic ; neovascularization ; Neovascularization, Physiologic ; Stem Cells ; transgenic mice ; vascular development ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; VEGF-A</subject><ispartof>Developmental biology, 2000-07, Vol.223 (2), p.383-398</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-507dd125438df4c84f5082deaa464342f3b1777c728fb30a1815c719cb6776ef3</citedby><cites>FETCH-LOGICAL-c380t-507dd125438df4c84f5082deaa464342f3b1777c728fb30a1815c719cb6776ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/dbio.2000.9755$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10882523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ash, John D.</creatorcontrib><creatorcontrib>Overbeek, Paul A.</creatorcontrib><title>Lens-Specific VEGF-A Expression Induces Angioblast Migration and Proliferation and Stimulates Angiogenic Remodeling</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Vascular endothelial growth factor (VEGF) is a secreted mitogen which specifically stimulates proliferation of vascular endothelial cells in vitro and in vivo. Its expression pattern is consistent with it being an important regulator of vasculogenesis and angiogenesis, and targeted disruption of VEGF-A has demonstrated that it is essential for vascular development. To determine if VEGF-A was sufficient to alter vascularization in the eye we generated transgenic mice which express human VEGF-A165 specifically in the lens. Expression of transgenic VEGF-A led to excessive proliferation and accumulation of disorganized angioblasts and endothelial cells around the lens. The results support the hypothesis that VEGF-A can initiate the process of vascularization by stimulating chemoattraction and proliferation of angioblasts and endothelial cells and that VEGF-A expression can stimulate angiogenic remodeling. However, VEGF-A alone was not sufficient to direct blood vessel organization or maturation.</description><subject>angioblasts</subject><subject>angiogenesis</subject><subject>Animals</subject><subject>Basement Membrane - metabolism</subject><subject>Collagen - biosynthesis</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Endothelium, Vascular - cytology</subject><subject>Eye - blood supply</subject><subject>Eye - embryology</subject><subject>Eye - growth & development</subject><subject>eye development</subject><subject>Humans</subject><subject>Hyperplasia</subject><subject>Laminin - biosynthesis</subject><subject>Lens, Crystalline - blood supply</subject><subject>Lens, Crystalline - embryology</subject><subject>Lens, Crystalline - growth & development</subject><subject>Lymphokines - genetics</subject><subject>Mesoderm - cytology</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>neovascularization</subject><subject>Neovascularization, Physiologic</subject><subject>Stem Cells</subject><subject>transgenic mice</subject><subject>vascular development</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><subject>VEGF-A</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFv1DAQRi0EotvClSPKiVuWsR3HznFVbUulrUAUEDfLsccro8Re7ATBvyfRFqkXTiPNvO-T5hHyhsKWArTvXR_SlgHAtpNCPCMbCp2oRdt8f042AJTVtIX2glyW8mOhuFL8JbmgoBQTjG9IOWAs9cMJbfDBVt_2tzf1rtr_PmUsJaRY3UU3WyzVLh5D6gdTpuo-HLOZ1qOJrvqU0xA8Ptk8TGGcBzP9Sx0xLtWfcUwOhxCPr8gLb4aCrx_nFfl6s_9y_aE-fLy9u94dassVTLUA6RxlouHK-caqxgtQzKExTdvwhnneUymllUz5noOhigoraWf7VsoWPb8i7869p5x-zlgmPYZicRhMxDQXLSnjHLpuAbdn0OZUSkavTzmMJv_RFPSqWa-a9apZr5qXwNvH5rkf0T3Bz14XQJ0BXP77FTDrYgNGiy5ktJN2Kfyv-y8LM4yh</recordid><startdate>20000715</startdate><enddate>20000715</enddate><creator>Ash, John D.</creator><creator>Overbeek, Paul A.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000715</creationdate><title>Lens-Specific VEGF-A Expression Induces Angioblast Migration and Proliferation and Stimulates Angiogenic Remodeling</title><author>Ash, John D. ; Overbeek, Paul A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-507dd125438df4c84f5082deaa464342f3b1777c728fb30a1815c719cb6776ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>angioblasts</topic><topic>angiogenesis</topic><topic>Animals</topic><topic>Basement Membrane - metabolism</topic><topic>Collagen - biosynthesis</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Endothelium, Vascular - cytology</topic><topic>Eye - blood supply</topic><topic>Eye - embryology</topic><topic>Eye - growth & development</topic><topic>eye development</topic><topic>Humans</topic><topic>Hyperplasia</topic><topic>Laminin - biosynthesis</topic><topic>Lens, Crystalline - blood supply</topic><topic>Lens, Crystalline - embryology</topic><topic>Lens, Crystalline - growth & development</topic><topic>Lymphokines - genetics</topic><topic>Mesoderm - cytology</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>neovascularization</topic><topic>Neovascularization, Physiologic</topic><topic>Stem Cells</topic><topic>transgenic mice</topic><topic>vascular development</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><topic>VEGF-A</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ash, John D.</creatorcontrib><creatorcontrib>Overbeek, Paul A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ash, John D.</au><au>Overbeek, Paul A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lens-Specific VEGF-A Expression Induces Angioblast Migration and Proliferation and Stimulates Angiogenic Remodeling</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2000-07-15</date><risdate>2000</risdate><volume>223</volume><issue>2</issue><spage>383</spage><epage>398</epage><pages>383-398</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Vascular endothelial growth factor (VEGF) is a secreted mitogen which specifically stimulates proliferation of vascular endothelial cells in vitro and in vivo. Its expression pattern is consistent with it being an important regulator of vasculogenesis and angiogenesis, and targeted disruption of VEGF-A has demonstrated that it is essential for vascular development. To determine if VEGF-A was sufficient to alter vascularization in the eye we generated transgenic mice which express human VEGF-A165 specifically in the lens. Expression of transgenic VEGF-A led to excessive proliferation and accumulation of disorganized angioblasts and endothelial cells around the lens. The results support the hypothesis that VEGF-A can initiate the process of vascularization by stimulating chemoattraction and proliferation of angioblasts and endothelial cells and that VEGF-A expression can stimulate angiogenic remodeling. 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| ispartof | Developmental biology, 2000-07, Vol.223 (2), p.383-398 |
| issn | 0012-1606 1095-564X |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | angioblasts angiogenesis Animals Basement Membrane - metabolism Collagen - biosynthesis Endothelial Growth Factors - genetics Endothelium, Vascular - cytology Eye - blood supply Eye - embryology Eye - growth & development eye development Humans Hyperplasia Laminin - biosynthesis Lens, Crystalline - blood supply Lens, Crystalline - embryology Lens, Crystalline - growth & development Lymphokines - genetics Mesoderm - cytology Mice Mice, Transgenic neovascularization Neovascularization, Physiologic Stem Cells transgenic mice vascular development Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors VEGF-A |
| title | Lens-Specific VEGF-A Expression Induces Angioblast Migration and Proliferation and Stimulates Angiogenic Remodeling |
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