Direct determination of the kinetics of oxygen diffusion to the photocytes of a bioluminescent elaterid larva, measurement of gas- and aqueous-phase diffusional barriers and modelling of oxygen supply
We describe the development and use of a direct kinetic technique to determine the time taken for oxygen to diffuse from the external environment into the light-producing cells (photocytes) in the prothorax of bioluminescent larvae of Pyrearinus termitilluminans. This was achieved by measuring the t...
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Veröffentlicht in: | Journal of experimental biology 2000-08, Vol.203 (Pt 16), p.2479-2484 |
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creator | Timmins, G S Bechara, E J Swartz, H M |
description | We describe the development and use of a direct kinetic technique to determine the time taken for oxygen to diffuse from the external environment into the light-producing cells (photocytes) in the prothorax of bioluminescent larvae of Pyrearinus termitilluminans. This was achieved by measuring the time course of the pseudoflash induced through sequential anoxia followed by normoxia. We have also determined the separate times taken for this oxygen diffusion in gaseous and tissue (predominantly aqueous) phases by using helium and nitrogen as the carrier gas. Of the total time taken for diffusion, that in the gas phase required 613+/-136 ms (mean +/- s.e. m., N=5) whilst that in the aqueous phase required 1313+/-187 ms. These values imply pathlengths of diffusion in the gaseous and aqueous phases of 4.80x10(-)(3)+/-0.53x10(-)(3) and 8. 89x10(-)(5)+/-0.61x10(-)(5 )m, respectively. In addition, the pathlength of gas-phase diffusion was used to derive a parameter relating to the tortuosity of the tracheal system. These values, together with those obtained upon bioluminescent oxygen consumption, have been used to model oxygen supply to the photocyte. From these studies, it would also appear that the modulation of tracheolar fluid levels might be a significant mechanism of control of tissue oxygen levels in at least some insects. |
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This was achieved by measuring the time course of the pseudoflash induced through sequential anoxia followed by normoxia. We have also determined the separate times taken for this oxygen diffusion in gaseous and tissue (predominantly aqueous) phases by using helium and nitrogen as the carrier gas. Of the total time taken for diffusion, that in the gas phase required 613+/-136 ms (mean +/- s.e. m., N=5) whilst that in the aqueous phase required 1313+/-187 ms. These values imply pathlengths of diffusion in the gaseous and aqueous phases of 4.80x10(-)(3)+/-0.53x10(-)(3) and 8. 89x10(-)(5)+/-0.61x10(-)(5 )m, respectively. In addition, the pathlength of gas-phase diffusion was used to derive a parameter relating to the tortuosity of the tracheal system. These values, together with those obtained upon bioluminescent oxygen consumption, have been used to model oxygen supply to the photocyte. 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This was achieved by measuring the time course of the pseudoflash induced through sequential anoxia followed by normoxia. We have also determined the separate times taken for this oxygen diffusion in gaseous and tissue (predominantly aqueous) phases by using helium and nitrogen as the carrier gas. Of the total time taken for diffusion, that in the gas phase required 613+/-136 ms (mean +/- s.e. m., N=5) whilst that in the aqueous phase required 1313+/-187 ms. These values imply pathlengths of diffusion in the gaseous and aqueous phases of 4.80x10(-)(3)+/-0.53x10(-)(3) and 8. 89x10(-)(5)+/-0.61x10(-)(5 )m, respectively. In addition, the pathlength of gas-phase diffusion was used to derive a parameter relating to the tortuosity of the tracheal system. These values, together with those obtained upon bioluminescent oxygen consumption, have been used to model oxygen supply to the photocyte. From these studies, it would also appear that the modulation of tracheolar fluid levels might be a significant mechanism of control of tissue oxygen levels in at least some insects.</description><subject>Animals</subject><subject>Coleoptera - cytology</subject><subject>Coleoptera - growth & development</subject><subject>Coleoptera - metabolism</subject><subject>Diffusion</subject><subject>Kinetics</subject><subject>Larva - cytology</subject><subject>Larva - metabolism</subject><subject>Luminescent Measurements</subject><subject>Models, Biological</subject><subject>Oxygen - metabolism</subject><subject>Pyrearinus termitilluminans</subject><issn>0022-0949</issn><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi1ERYfCmh3yihWZ-i9xskSFFqRK3ZR1dJNcz7g4cbCdinlDHgtnphLd1Qtblo8_3-tDyAfOtlwocfmA3VYwueXVVijdvCIbrrQuGq7K12TDmBAFa1RzTt7G-MDyqEr1hpxz1jDJK7Ehf7_agH2iAyYMo50gWT9Rb2jaI_1lJ0y2j-ve_znscKKDNWaJK5P8kZn3Pvn-kPBIAe2sd0sOwtjjlCg6yMF2oA7CI3ymI0JcAo7rWeZ3EAsK00Dh94J-icW8h4j_XwFHOwjBYohHbPQDOmen3bOS4jLP7vCOnBlwEd8_rRfk5_W3-6vvxe3dzY-rL7dFr5hMRcegUrXC2hiOXf5CwxQHXeWZ11yUUhgp667s69oI0wvgJehS1xXTpmxAygvy6ZQ7B59rjqkdbW7VOZjWBlrNhRQ1r18Eua4axvQKXp7APvgYA5p2DnaEcGg5a1fLbbbcZsstr9rVcr7x8Sl66UYcnvEnrfIfQLKnqQ</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Timmins, G S</creator><creator>Bechara, E J</creator><creator>Swartz, H M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Direct determination of the kinetics of oxygen diffusion to the photocytes of a bioluminescent elaterid larva, measurement of gas- and aqueous-phase diffusional barriers and modelling of oxygen supply</title><author>Timmins, G S ; Bechara, E J ; Swartz, H M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-b0a6484e8ff1eb242f041a760411812532f338b5c88f2fc2a15a7578607f59a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Coleoptera - cytology</topic><topic>Coleoptera - growth & development</topic><topic>Coleoptera - metabolism</topic><topic>Diffusion</topic><topic>Kinetics</topic><topic>Larva - cytology</topic><topic>Larva - metabolism</topic><topic>Luminescent Measurements</topic><topic>Models, Biological</topic><topic>Oxygen - metabolism</topic><topic>Pyrearinus termitilluminans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Timmins, G S</creatorcontrib><creatorcontrib>Bechara, E J</creatorcontrib><creatorcontrib>Swartz, H M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Timmins, G S</au><au>Bechara, E J</au><au>Swartz, H M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct determination of the kinetics of oxygen diffusion to the photocytes of a bioluminescent elaterid larva, measurement of gas- and aqueous-phase diffusional barriers and modelling of oxygen supply</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>203</volume><issue>Pt 16</issue><spage>2479</spage><epage>2484</epage><pages>2479-2484</pages><issn>0022-0949</issn><eissn>1477-9145</eissn><abstract>We describe the development and use of a direct kinetic technique to determine the time taken for oxygen to diffuse from the external environment into the light-producing cells (photocytes) in the prothorax of bioluminescent larvae of Pyrearinus termitilluminans. This was achieved by measuring the time course of the pseudoflash induced through sequential anoxia followed by normoxia. We have also determined the separate times taken for this oxygen diffusion in gaseous and tissue (predominantly aqueous) phases by using helium and nitrogen as the carrier gas. Of the total time taken for diffusion, that in the gas phase required 613+/-136 ms (mean +/- s.e. m., N=5) whilst that in the aqueous phase required 1313+/-187 ms. These values imply pathlengths of diffusion in the gaseous and aqueous phases of 4.80x10(-)(3)+/-0.53x10(-)(3) and 8. 89x10(-)(5)+/-0.61x10(-)(5 )m, respectively. In addition, the pathlength of gas-phase diffusion was used to derive a parameter relating to the tortuosity of the tracheal system. These values, together with those obtained upon bioluminescent oxygen consumption, have been used to model oxygen supply to the photocyte. From these studies, it would also appear that the modulation of tracheolar fluid levels might be a significant mechanism of control of tissue oxygen levels in at least some insects.</abstract><cop>England</cop><pmid>10903162</pmid><doi>10.1242/jeb.203.16.2479</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Coleoptera - cytology Coleoptera - growth & development Coleoptera - metabolism Diffusion Kinetics Larva - cytology Larva - metabolism Luminescent Measurements Models, Biological Oxygen - metabolism Pyrearinus termitilluminans |
title | Direct determination of the kinetics of oxygen diffusion to the photocytes of a bioluminescent elaterid larva, measurement of gas- and aqueous-phase diffusional barriers and modelling of oxygen supply |
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