Evidence that ganglioside enriched domains are distinct from caveolae in MDCK II and human fibroblast cells in culture

Cultures of MDCK II and human fibroblast cells were fed radioactive sphingosine and a radioactive GM3 ganglioside derivative containing a photoactivable group. The derived cell homogenates were treated with Triton X‐100 and fractionated by sucrose‐gradient centrifugation to prepare a detergent‐insol...

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Veröffentlicht in:	European journal of biochemistry 2000-07, Vol.267 (13), p.4187-4197
Hauptverfasser:	Chigorno, Vanna, Palestini, Paola, Sciannamblo, Mariateresa, Dolo, Vincenza, Pavan, Antonio, Tettamanti, Guido, Sonnino, Sandro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals caveolae caveolin‐1 Cell Membrane - chemistry Cells, Cultured Electrophoresis, Polyacrylamide Gel Fibroblasts - chemistry gangliosides Gangliosides - analysis GM3 Humans Molecular Weight sphingolipid domains Sphingosine - metabolism
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