Expression of the Arabidopsis thaliana AtJ2 Cochaperone Protein in Pichia pastoris
A vector was constructed for intracellular expression of the Arabidopsis thaliana DnaJ homologue AtJ2 in the methylotrophic yeast Pichia pastoris. The vector includes DNA encoding an amino-terminal histidine-tag, to simplify protein purification. Shake-flask cultures could be induced to produce appr...
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| Veröffentlicht in: | Protein expression and purification 2000-07, Vol.19 (2), p.253-258 |
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| container_title | Protein expression and purification |
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| creator | Zhou, Rengang Kroczyńska, Barbara Miernyk, Jan A. |
| description | A vector was constructed for intracellular expression of the Arabidopsis thaliana DnaJ homologue AtJ2 in the methylotrophic yeast Pichia pastoris. The vector includes DNA encoding an amino-terminal histidine-tag, to simplify protein purification. Shake-flask cultures could be induced to produce approximately 250 mg/ L of AtJ2. Purified recombinant AtJ2 was able to stimulate the ATPase activities of both the Escherichia coli and Zea mays cytoplasmic Stress70 chaperone proteins five- to ninefold. The carboxy terminus of AtJ2 is –CAQQ, a protein farnesylation motif. When transformed P. pastoris was induced to synthesize AtJ2 in the presence of [3H]mevalonolactone, radioactivity was incorporated into the protein, suggesting farnesylation. |
| doi_str_mv | 10.1006/prep.2000.1254 |
| format | Article |
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The vector includes DNA encoding an amino-terminal histidine-tag, to simplify protein purification. Shake-flask cultures could be induced to produce approximately 250 mg/ L of AtJ2. Purified recombinant AtJ2 was able to stimulate the ATPase activities of both the Escherichia coli and Zea mays cytoplasmic Stress70 chaperone proteins five- to ninefold. The carboxy terminus of AtJ2 is –CAQQ, a protein farnesylation motif. 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| ispartof | Protein expression and purification, 2000-07, Vol.19 (2), p.253-258 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphatases - chemistry Arabidopsis - chemistry Arabidopsis Proteins Escherichia coli Proteins Heat-Shock Proteins - chemistry Heat-Shock Proteins - isolation & purification Heat-Shock Proteins - metabolism Histidine - chemistry HSP40 Heat-Shock Proteins Molecular Chaperones - metabolism Pichia - chemistry Pichia - metabolism Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism |
| title | Expression of the Arabidopsis thaliana AtJ2 Cochaperone Protein in Pichia pastoris |
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