Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin
Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography...
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Veröffentlicht in: | Insect biochemistry and molecular biology 2000-09, Vol.30 (8), p.681-689 |
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creator | Audsley, Neil Weaver, Robert J Edwards, John P |
description | Juvenile hormone (JH) biosynthesis and the effects of synthetic
Manduca sexta allatostatin (Mas-AS) and
M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth,
Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200
fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval
L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in
L. oleracea. |
doi_str_mv | 10.1016/S0965-1748(00)00039-4 |
format | Article |
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Manduca sexta allatostatin (Mas-AS) and
M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth,
Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200
fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval
L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in
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Manduca sexta allatostatin (Mas-AS) and
M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth,
Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200
fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval
L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in
L. oleracea.</description><subject>Allatostatin</subject><subject>Allatotropin</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Corpora allata</subject><subject>Corpora Allata - metabolism</subject><subject>esterase</subject><subject>Hormone Antagonists - metabolism</subject><subject>Insect</subject><subject>Insect Hormones - metabolism</subject><subject>Juvenile hormone</subject><subject>Juvenile hormone acid</subject><subject>Juvenile Hormones - biosynthesis</subject><subject>Lacanobia</subject><subject>Lacanobia oleracea</subject><subject>Larva - metabolism</subject><subject>Lycopersicon esculentum</subject><subject>Manduca</subject><subject>Manduca - metabolism</subject><subject>Manduca sexta</subject><subject>Molecular Sequence Data</subject><subject>Moths - metabolism</subject><subject>Neuropeptide</subject><subject>Neuropeptides - metabolism</subject><subject>Noctuidae</subject><subject>Tissue Extracts - metabolism</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoMo7uzqT1ByJS5sNWnTprlaZPGTES_U63CanjqRNhmTdHD-iT_XdDqId3sVOHne94Q8hDzj7BVnvHn9lammLrgU7UvGrhljlSrEA7LhrVQFKwV7SDb_kAtyGePPDAlRy8fkgrNWNpzzDfnzaT6gsyPSnQ-Td0g76-PRpR1GG2l3pMaHvQ9AYRwhAfUDHSEcYKTJT5A8nXza3dAtGHC-sxkYMYBBuKHgehrwx5xz1rul63MezQZoxN_p3OhjytfuBK-DFPzeuifk0QBjxKfn84p8f_f2292HYvvl_ce7N9vCiLpNhWoHpZSo-0rVoDrolRQDKNXLtuo60dStKVshZF8K5LIuKwHQmqapuJJoelFdkRdr7z74XzPGpCcbDeaXOPRz1JKXZatUcy-Y2-sML431CprgYww46H2wE4Sj5kwv7vTJnV7EaMb0yZ1ecs_PC-Zuwv6_1CorA7crgPk_DhaDjsaiM9jbgCbp3tt7VvwFCz-rpw</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Audsley, Neil</creator><creator>Weaver, Robert J</creator><creator>Edwards, John P</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>200009</creationdate><title>Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin</title><author>Audsley, Neil ; Weaver, Robert J ; Edwards, John P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-98f99945d395a9bad974fa99d783bb4658c28447d24e175234aa8c663197ecd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Allatostatin</topic><topic>Allatotropin</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Corpora allata</topic><topic>Corpora Allata - metabolism</topic><topic>esterase</topic><topic>Hormone Antagonists - metabolism</topic><topic>Insect</topic><topic>Insect Hormones - metabolism</topic><topic>Juvenile hormone</topic><topic>Juvenile hormone acid</topic><topic>Juvenile Hormones - biosynthesis</topic><topic>Lacanobia</topic><topic>Lacanobia oleracea</topic><topic>Larva - metabolism</topic><topic>Lycopersicon esculentum</topic><topic>Manduca</topic><topic>Manduca - metabolism</topic><topic>Manduca sexta</topic><topic>Molecular Sequence Data</topic><topic>Moths - metabolism</topic><topic>Neuropeptide</topic><topic>Neuropeptides - metabolism</topic><topic>Noctuidae</topic><topic>Tissue Extracts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Audsley, Neil</creatorcontrib><creatorcontrib>Weaver, Robert J</creatorcontrib><creatorcontrib>Edwards, John P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Audsley, Neil</au><au>Weaver, Robert J</au><au>Edwards, John P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2000-09</date><risdate>2000</risdate><volume>30</volume><issue>8</issue><spage>681</spage><epage>689</epage><pages>681-689</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Juvenile hormone (JH) biosynthesis and the effects of synthetic
Manduca sexta allatostatin (Mas-AS) and
M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth,
Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200
fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval
L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in
L. oleracea.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>10876111</pmid><doi>10.1016/S0965-1748(00)00039-4</doi><tpages>9</tpages></addata></record> |
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subjects | Allatostatin Allatotropin Amino Acid Sequence Animals Brain - metabolism Carboxylic Ester Hydrolases - metabolism Corpora allata Corpora Allata - metabolism esterase Hormone Antagonists - metabolism Insect Insect Hormones - metabolism Juvenile hormone Juvenile hormone acid Juvenile Hormones - biosynthesis Lacanobia Lacanobia oleracea Larva - metabolism Lycopersicon esculentum Manduca Manduca - metabolism Manduca sexta Molecular Sequence Data Moths - metabolism Neuropeptide Neuropeptides - metabolism Noctuidae Tissue Extracts - metabolism |
title | Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin |
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