Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin

Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography...

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Veröffentlicht in:Insect biochemistry and molecular biology 2000-09, Vol.30 (8), p.681-689
Hauptverfasser: Audsley, Neil, Weaver, Robert J, Edwards, John P
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Weaver, Robert J
Edwards, John P
description Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200 fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in L. oleracea.
doi_str_mv 10.1016/S0965-1748(00)00039-4
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Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200 fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval L. oleracea brain homogenates. 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Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100–200 fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval L. oleracea brain homogenates. 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Weaver, Robert J ; Edwards, John P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-98f99945d395a9bad974fa99d783bb4658c28447d24e175234aa8c663197ecd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Allatostatin</topic><topic>Allatotropin</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Corpora allata</topic><topic>Corpora Allata - metabolism</topic><topic>esterase</topic><topic>Hormone Antagonists - metabolism</topic><topic>Insect</topic><topic>Insect Hormones - metabolism</topic><topic>Juvenile hormone</topic><topic>Juvenile hormone acid</topic><topic>Juvenile Hormones - biosynthesis</topic><topic>Lacanobia</topic><topic>Lacanobia oleracea</topic><topic>Larva - metabolism</topic><topic>Lycopersicon esculentum</topic><topic>Manduca</topic><topic>Manduca - metabolism</topic><topic>Manduca sexta</topic><topic>Molecular Sequence Data</topic><topic>Moths - metabolism</topic><topic>Neuropeptide</topic><topic>Neuropeptides - metabolism</topic><topic>Noctuidae</topic><topic>Tissue Extracts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Audsley, Neil</creatorcontrib><creatorcontrib>Weaver, Robert J</creatorcontrib><creatorcontrib>Edwards, John P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Audsley, Neil</au><au>Weaver, Robert J</au><au>Edwards, John P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2000-09</date><risdate>2000</risdate><volume>30</volume><issue>8</issue><spage>681</spage><epage>689</epage><pages>681-689</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. 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subjects Allatostatin
Allatotropin
Amino Acid Sequence
Animals
Brain - metabolism
Carboxylic Ester Hydrolases - metabolism
Corpora allata
Corpora Allata - metabolism
esterase
Hormone Antagonists - metabolism
Insect
Insect Hormones - metabolism
Juvenile hormone
Juvenile hormone acid
Juvenile Hormones - biosynthesis
Lacanobia
Lacanobia oleracea
Larva - metabolism
Lycopersicon esculentum
Manduca
Manduca - metabolism
Manduca sexta
Molecular Sequence Data
Moths - metabolism
Neuropeptide
Neuropeptides - metabolism
Noctuidae
Tissue Extracts - metabolism
title Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin
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