Regionally Distinct Regulation of Astroglial Neurotransmitter Receptors by Fibroblast Growth Factor-2
Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19–30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-rece...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2000-07, Vol.16 (1), p.42-58 |
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| creator | Reuss, Bernhard Leung, Doreen S.Y. Ohlemeyer, Carsten Kettenmann, Helmut Unsicker, Klaus |
| description | Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19–30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]i. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. These results indicate that downregulation of astroglial gap junctions by FGF-2 is accompanied by an upregulation of D1DR and DA sensitivity, adding a new aspect to the role of FGF-2 in the regulation of brain functions. |
| doi_str_mv | 10.1006/mcne.2000.0857 |
| format | Article |
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We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19–30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]i. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. 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We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19–30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]i. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. These results indicate that downregulation of astroglial gap junctions by FGF-2 is accompanied by an upregulation of D1DR and DA sensitivity, adding a new aspect to the role of FGF-2 in the regulation of brain functions.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Calcium - metabolism</subject><subject>Cell Count - drug effects</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Corpus Striatum - cytology</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>Dopamine - metabolism</subject><subject>Dopamine - pharmacology</subject><subject>Dopamine Agonists - pharmacology</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Gap Junctions - drug effects</subject><subject>Gap Junctions - metabolism</subject><subject>Glutamic Acid - metabolism</subject><subject>Glutamic Acid - pharmacology</subject><subject>Glycyrrhetinic Acid - analogs & derivatives</subject><subject>Glycyrrhetinic Acid - pharmacology</subject><subject>Intracellular Fluid - drug effects</subject><subject>Intracellular Fluid - metabolism</subject><subject>Mesencephalon - cytology</subject><subject>Mesencephalon - drug effects</subject><subject>Mesencephalon - metabolism</subject><subject>Octanols - pharmacology</subject><subject>Rats</subject><subject>Receptors, Dopamine D1 - agonists</subject><subject>Receptors, Dopamine D1 - biosynthesis</subject><subject>Receptors, Dopamine D1 - genetics</subject><subject>Receptors, Neurotransmitter - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQha2qqFDaa4-VT71lO3ac2DkiYKESKhKCs-U4E-rKiRfbKdp_j6PlwAX1NKM337zDe4R8Y7BhAO3Pyc644QCwAdXID-SEQddUXc3lx3UXopKiZsfkc0p_C9Xwrv5EjhkoxYXiJwTv8NGF2Xi_pxcuZTfbTIu2eJOLTsNIz1KO4dE74-lvXGLI0cxpcjljLKTFXQ4x0X5Pt66PofcmZXoVw3P-Q7fGlmPFv5Cj0fiEX1_nKXnYXt6fX1c3t1e_zs9uKitqmSsGVgDrLZOj6hur2pEPhg8w4sAROROCIXRiGAVH1vZQlKZlXd9CB2NnVH1Kfhx8dzE8LZiynlyy6L2ZMSxJS8a5ku3_QSaLZ8dlATcH0MaQUsRR76KbTNxrBnptQK8N6LUBvTZQHr6_Oi_9hMMb_BB5AdQBwBLEP4dRJ-twtji4iDbrIbj3vF8AMseV4Q</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Reuss, Bernhard</creator><creator>Leung, Doreen S.Y.</creator><creator>Ohlemeyer, Carsten</creator><creator>Kettenmann, Helmut</creator><creator>Unsicker, Klaus</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Regionally Distinct Regulation of Astroglial Neurotransmitter Receptors by Fibroblast Growth Factor-2</title><author>Reuss, Bernhard ; Leung, Doreen S.Y. ; Ohlemeyer, Carsten ; Kettenmann, Helmut ; Unsicker, Klaus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-10c401bc17f8b5c86f2da2d0fed2ee21441e094df42e16b02145619b6090f9a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Calcium - metabolism</topic><topic>Cell Count - drug effects</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Corpus Striatum - cytology</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>Dopamine - metabolism</topic><topic>Dopamine - pharmacology</topic><topic>Dopamine Agonists - pharmacology</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Gap Junctions - drug effects</topic><topic>Gap Junctions - metabolism</topic><topic>Glutamic Acid - metabolism</topic><topic>Glutamic Acid - pharmacology</topic><topic>Glycyrrhetinic Acid - analogs & derivatives</topic><topic>Glycyrrhetinic Acid - pharmacology</topic><topic>Intracellular Fluid - drug effects</topic><topic>Intracellular Fluid - metabolism</topic><topic>Mesencephalon - cytology</topic><topic>Mesencephalon - drug effects</topic><topic>Mesencephalon - metabolism</topic><topic>Octanols - pharmacology</topic><topic>Rats</topic><topic>Receptors, Dopamine D1 - agonists</topic><topic>Receptors, Dopamine D1 - biosynthesis</topic><topic>Receptors, Dopamine D1 - genetics</topic><topic>Receptors, Neurotransmitter - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reuss, Bernhard</creatorcontrib><creatorcontrib>Leung, Doreen S.Y.</creatorcontrib><creatorcontrib>Ohlemeyer, Carsten</creatorcontrib><creatorcontrib>Kettenmann, Helmut</creatorcontrib><creatorcontrib>Unsicker, Klaus</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reuss, Bernhard</au><au>Leung, Doreen S.Y.</au><au>Ohlemeyer, Carsten</au><au>Kettenmann, Helmut</au><au>Unsicker, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regionally Distinct Regulation of Astroglial Neurotransmitter Receptors by Fibroblast Growth Factor-2</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>16</volume><issue>1</issue><spage>42</spage><epage>58</epage><pages>42-58</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 downregulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19–30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]i. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. These results indicate that downregulation of astroglial gap junctions by FGF-2 is accompanied by an upregulation of D1DR and DA sensitivity, adding a new aspect to the role of FGF-2 in the regulation of brain functions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10882482</pmid><doi>10.1006/mcne.2000.0857</doi><tpages>17</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Astrocytes - cytology Astrocytes - drug effects Astrocytes - metabolism Calcium - metabolism Cell Count - drug effects Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - drug effects Cerebral Cortex - metabolism Corpus Striatum - cytology Corpus Striatum - drug effects Corpus Striatum - metabolism Dopamine - metabolism Dopamine - pharmacology Dopamine Agonists - pharmacology Fibroblast Growth Factor 2 - metabolism Fibroblast Growth Factor 2 - pharmacology Gap Junctions - drug effects Gap Junctions - metabolism Glutamic Acid - metabolism Glutamic Acid - pharmacology Glycyrrhetinic Acid - analogs & derivatives Glycyrrhetinic Acid - pharmacology Intracellular Fluid - drug effects Intracellular Fluid - metabolism Mesencephalon - cytology Mesencephalon - drug effects Mesencephalon - metabolism Octanols - pharmacology Rats Receptors, Dopamine D1 - agonists Receptors, Dopamine D1 - biosynthesis Receptors, Dopamine D1 - genetics Receptors, Neurotransmitter - metabolism RNA, Messenger - biosynthesis |
| title | Regionally Distinct Regulation of Astroglial Neurotransmitter Receptors by Fibroblast Growth Factor-2 |
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