ETHANOL INHIBITS SKELETAL MUSCLE CELL PROLIFERATION AND DELAYS ITS DIFFERENTIATION IN CELL CULTURE
Chronic ingestion of ethanol (EtOH) produces physiological and morphological alterations in skeletal muscle. The effects of EtOH on skeletal muscle have been studied in experimental animals or on biopsies from alcoholic patients. However, alterations in skeletal muscle from alcoholic patients could...
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| Veröffentlicht in: | Alcohol and alcoholism (Oxford) 2000-05, Vol.35 (3), p.236-241 |
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| container_title | Alcohol and alcoholism (Oxford) |
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| creator | GARRIGA, J. ADANERO, E. FERNÁNDEZ-SOLÁ, J. URBANO-MÁRQUEZ, A. CUSSÓ, R. |
| description | Chronic ingestion of ethanol (EtOH) produces physiological and morphological alterations in skeletal muscle. The effects of EtOH on skeletal muscle have been studied in experimental animals or on biopsies from alcoholic patients. However, alterations in skeletal muscle from alcoholic patients could be secondary to the effects of EtOH on the nervous system. In this study, by assaying the action of EtOH on primary skeletal muscle cell cultures, we provide evidence of its direct effect on skeletal muscle proliferation and differentiation. The results indicate that EtOH: (1) significantly inhibits skeletal muscle cell proliferation at the beginning of the proliferation phase; (2) delays skeletal muscle differentiation, shown by the significant changes in the evolution of the percentage of the creatine kinase isozymes; (3) has no significant effect on skeletal muscle DNA or protein content during the proliferation phase. |
| doi_str_mv | 10.1093/alcalc/35.3.236 |
| format | Article |
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The effects of EtOH on skeletal muscle have been studied in experimental animals or on biopsies from alcoholic patients. However, alterations in skeletal muscle from alcoholic patients could be secondary to the effects of EtOH on the nervous system. In this study, by assaying the action of EtOH on primary skeletal muscle cell cultures, we provide evidence of its direct effect on skeletal muscle proliferation and differentiation. The results indicate that EtOH: (1) significantly inhibits skeletal muscle cell proliferation at the beginning of the proliferation phase; (2) delays skeletal muscle differentiation, shown by the significant changes in the evolution of the percentage of the creatine kinase isozymes; (3) has no significant effect on skeletal muscle DNA or protein content during the proliferation phase.</description><identifier>ISSN: 0735-0414</identifier><identifier>ISSN: 1464-3502</identifier><identifier>EISSN: 1464-3502</identifier><identifier>DOI: 10.1093/alcalc/35.3.236</identifier><identifier>PMID: 10869241</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Alcoholism and acute alcohol poisoning ; Animals ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Division - drug effects ; Cell Division - physiology ; Cells, Cultured ; Central Nervous System Depressants - pharmacology ; Creatine Kinase - drug effects ; Creatine Kinase - physiology ; DNA - drug effects ; DNA - physiology ; Ethanol - pharmacology ; Isoenzymes ; Medical sciences ; Muscle Proteins - drug effects ; Muscle Proteins - physiology ; Muscle, Skeletal - cytology ; Muscle, Skeletal - drug effects ; Rats ; Rats, Sprague-Dawley ; Toxicology</subject><ispartof>Alcohol and alcoholism (Oxford), 2000-05, Vol.35 (3), p.236-241</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) May 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-e1b3373aa9df489921f11a06bf5c9c233ad9d2bdb048ee4206cabe620cb55e543</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1399818$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10869241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GARRIGA, J.</creatorcontrib><creatorcontrib>ADANERO, E.</creatorcontrib><creatorcontrib>FERNÁNDEZ-SOLÁ, J.</creatorcontrib><creatorcontrib>URBANO-MÁRQUEZ, A.</creatorcontrib><creatorcontrib>CUSSÓ, R.</creatorcontrib><title>ETHANOL INHIBITS SKELETAL MUSCLE CELL PROLIFERATION AND DELAYS ITS DIFFERENTIATION IN CELL CULTURE</title><title>Alcohol and alcoholism (Oxford)</title><addtitle>Alcohol and Alcoholism</addtitle><description>Chronic ingestion of ethanol (EtOH) produces physiological and morphological alterations in skeletal muscle. The effects of EtOH on skeletal muscle have been studied in experimental animals or on biopsies from alcoholic patients. However, alterations in skeletal muscle from alcoholic patients could be secondary to the effects of EtOH on the nervous system. In this study, by assaying the action of EtOH on primary skeletal muscle cell cultures, we provide evidence of its direct effect on skeletal muscle proliferation and differentiation. 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ADANERO, E. ; FERNÁNDEZ-SOLÁ, J. ; URBANO-MÁRQUEZ, A. ; CUSSÓ, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-e1b3373aa9df489921f11a06bf5c9c233ad9d2bdb048ee4206cabe620cb55e543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alcoholism and acute alcohol poisoning</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>Central Nervous System Depressants - pharmacology</topic><topic>Creatine Kinase - drug effects</topic><topic>Creatine Kinase - physiology</topic><topic>DNA - drug effects</topic><topic>DNA - physiology</topic><topic>Ethanol - pharmacology</topic><topic>Isoenzymes</topic><topic>Medical sciences</topic><topic>Muscle Proteins - drug effects</topic><topic>Muscle Proteins - physiology</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GARRIGA, J.</creatorcontrib><creatorcontrib>ADANERO, E.</creatorcontrib><creatorcontrib>FERNÁNDEZ-SOLÁ, J.</creatorcontrib><creatorcontrib>URBANO-MÁRQUEZ, A.</creatorcontrib><creatorcontrib>CUSSÓ, R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Alcohol and alcoholism (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GARRIGA, J.</au><au>ADANERO, E.</au><au>FERNÁNDEZ-SOLÁ, J.</au><au>URBANO-MÁRQUEZ, A.</au><au>CUSSÓ, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ETHANOL INHIBITS SKELETAL MUSCLE CELL PROLIFERATION AND DELAYS ITS DIFFERENTIATION IN CELL CULTURE</atitle><jtitle>Alcohol and alcoholism (Oxford)</jtitle><addtitle>Alcohol and Alcoholism</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>35</volume><issue>3</issue><spage>236</spage><epage>241</epage><pages>236-241</pages><issn>0735-0414</issn><issn>1464-3502</issn><eissn>1464-3502</eissn><abstract>Chronic ingestion of ethanol (EtOH) produces physiological and morphological alterations in skeletal muscle. 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| source | MEDLINE; Oxford Academic Journals (OUP); Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Alcoholism and acute alcohol poisoning Animals Biological and medical sciences Cell Differentiation - drug effects Cell Differentiation - physiology Cell Division - drug effects Cell Division - physiology Cells, Cultured Central Nervous System Depressants - pharmacology Creatine Kinase - drug effects Creatine Kinase - physiology DNA - drug effects DNA - physiology Ethanol - pharmacology Isoenzymes Medical sciences Muscle Proteins - drug effects Muscle Proteins - physiology Muscle, Skeletal - cytology Muscle, Skeletal - drug effects Rats Rats, Sprague-Dawley Toxicology |
| title | ETHANOL INHIBITS SKELETAL MUSCLE CELL PROLIFERATION AND DELAYS ITS DIFFERENTIATION IN CELL CULTURE |
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