Structural Characterization of Proteins with an Attached ATCUN Motif by Paramagnetic Relaxation Enhancement NMR Spectroscopy

The use of a short, three-residue Cu2+-binding sequence, the ATCUN motif, is presented as an approach for extracting long-range distance restraints from relaxation enhancement NMR spectroscopy. The ATCUN motif is prepended to the N-termini of proteins and binds Cu2+ with a very high affinity. Relaxa...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of the American Chemical Society 2001-10, Vol.123 (40), p.9843-9847
Hauptverfasser:	Donaldson, Logan W, Skrynnikov, Nikolai R, Choy, Wing-Yiu, Muhandiram, D. Ranjith, Sarkar, Bibudhendra, Forman-Kay, Julie D, Kay, Lewis E
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Motifs Binding Sites Copper - chemistry Humans Nuclear Magnetic Resonance, Biomolecular Protein Conformation Thermodynamics Ubiquitin - chemistry Ubiquitin - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!