Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes
Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHβ) and chorionic gonadotropin (CGβ) and their translation into protein in various leukocyte...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2000-04, Vol.162 (1), p.79-85 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Hotakainen, P.Kristina Serlachius, E.Martina Lintula, Susanna I. Alfthan, Henrik V. Schröder, Jim P. Stenman, Ulf-Håkan E. |
| description | Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHβ) and chorionic gonadotropin (CGβ) and their translation into protein in various leukocyte subsets. Monocytes, granulocytes, B and T-cells from peripheral blood were separated. Lymphocytes were stimulated with various mitogens, prolactin and mixed lymphocyte culture. LHβ and CGβ mRNA expression was determined by reverse transcriptase polymerase chain reaction. LH, LHβ, CG and CGβ protein were determined in the culture medium by immunofluorometric assays. LHβ mRNA expression was detected in all cell fractions and cultures and stimulation with prolactin induced LH protein in the culture medium. CGβ mRNA expression appeared after culture of lymphocytes, but mitogens and prolactin had no clear stimulating effect. The LH expression in leukocytes shown here suggests an autocrine function of this hormone in blood cells. |
| doi_str_mv | 10.1016/S0303-7207(00)00208-2 |
| format | Article |
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We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHβ) and chorionic gonadotropin (CGβ) and their translation into protein in various leukocyte subsets. Monocytes, granulocytes, B and T-cells from peripheral blood were separated. Lymphocytes were stimulated with various mitogens, prolactin and mixed lymphocyte culture. LHβ and CGβ mRNA expression was determined by reverse transcriptase polymerase chain reaction. LH, LHβ, CG and CGβ protein were determined in the culture medium by immunofluorometric assays. LHβ mRNA expression was detected in all cell fractions and cultures and stimulation with prolactin induced LH protein in the culture medium. CGβ mRNA expression appeared after culture of lymphocytes, but mitogens and prolactin had no clear stimulating effect. The LH expression in leukocytes shown here suggests an autocrine function of this hormone in blood cells.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/S0303-7207(00)00208-2</identifier><identifier>PMID: 10854700</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adult ; Base Sequence ; Cell Line ; Chorionic gonadotropin ; Chorionic Gonadotropin, beta Subunit, Human - blood ; Chorionic Gonadotropin, beta Subunit, Human - genetics ; Chorionic Gonadotropin, beta Subunit, Human - metabolism ; DNA Primers - genetics ; Female ; Gene Expression ; Humans ; In Vitro Techniques ; Leukocytes ; Leukocytes - metabolism ; Luteinising hormone ; Luteinizing Hormone - blood ; Luteinizing Hormone - genetics ; Luteinizing Hormone - metabolism ; Male ; Prolactin ; RNA, Messenger - blood ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Molecular and cellular endocrinology, 2000-04, Vol.162 (1), p.79-85</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-ee89ea1fe395f6f99f38e4dbde8dff6efe583ac73c96d5d89b9036c60f66f2f53</citedby><cites>FETCH-LOGICAL-c361t-ee89ea1fe395f6f99f38e4dbde8dff6efe583ac73c96d5d89b9036c60f66f2f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0303-7207(00)00208-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10854700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hotakainen, P.Kristina</creatorcontrib><creatorcontrib>Serlachius, E.Martina</creatorcontrib><creatorcontrib>Lintula, Susanna I.</creatorcontrib><creatorcontrib>Alfthan, Henrik V.</creatorcontrib><creatorcontrib>Schröder, Jim P.</creatorcontrib><creatorcontrib>Stenman, Ulf-Håkan E.</creatorcontrib><title>Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHβ) and chorionic gonadotropin (CGβ) and their translation into protein in various leukocyte subsets. Monocytes, granulocytes, B and T-cells from peripheral blood were separated. Lymphocytes were stimulated with various mitogens, prolactin and mixed lymphocyte culture. LHβ and CGβ mRNA expression was determined by reverse transcriptase polymerase chain reaction. LH, LHβ, CG and CGβ protein were determined in the culture medium by immunofluorometric assays. LHβ mRNA expression was detected in all cell fractions and cultures and stimulation with prolactin induced LH protein in the culture medium. CGβ mRNA expression appeared after culture of lymphocytes, but mitogens and prolactin had no clear stimulating effect. The LH expression in leukocytes shown here suggests an autocrine function of this hormone in blood cells.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Chorionic gonadotropin</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - blood</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - genetics</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - metabolism</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Leukocytes</subject><subject>Leukocytes - metabolism</subject><subject>Luteinising hormone</subject><subject>Luteinizing Hormone - blood</subject><subject>Luteinizing Hormone - genetics</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Male</subject><subject>Prolactin</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYNY7Fr9CEqepD5MvZnsZDJPUkr_CEVB2-eQSW52ozPJmMxI-w382GZ3i_gmBELgd869OYeQNwzOGDDx4Rtw4FVbQ3sK8B6gBlnVz8iKybauJDTtc7L6ixyTlzl_B4C2qeULcsxANusWYEV-Xz5MCXP2MdDo6LDM6IPPPmzoNqYxBqQ6WGrKoyDe0E0M2sY5xckH2uOsq7z0S_AzHYsNhg2m6uvn871qSnFnR8vZLqMOdMLkpy0mPdB-iNHSAZcf0TzOmF-RI6eHjK-f7hNyf3V5d3FT3X65_nRxflsZLthcIcoONXPIu8YJ13WOS1zb3qK0zgl02EiuTctNJ2xjZdd3wIUR4IRwtWv4CXl38C3L_Vwwz2r02eAw6IBxyapldYlyzQvYHECTYs4JnZqSH3V6VAzUrgK1r0Dt8lUAal-Bqovu7dOApR_R_qM6ZF6AjwcAyzd_eUwqG4_BoPUJzaxs9P8Z8Qfv-pp-</recordid><startdate>20000425</startdate><enddate>20000425</enddate><creator>Hotakainen, P.Kristina</creator><creator>Serlachius, E.Martina</creator><creator>Lintula, Susanna I.</creator><creator>Alfthan, Henrik V.</creator><creator>Schröder, Jim P.</creator><creator>Stenman, Ulf-Håkan E.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000425</creationdate><title>Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes</title><author>Hotakainen, P.Kristina ; Serlachius, E.Martina ; Lintula, Susanna I. ; Alfthan, Henrik V. ; Schröder, Jim P. ; Stenman, Ulf-Håkan E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-ee89ea1fe395f6f99f38e4dbde8dff6efe583ac73c96d5d89b9036c60f66f2f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Chorionic gonadotropin</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - blood</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - genetics</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - metabolism</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Leukocytes</topic><topic>Leukocytes - metabolism</topic><topic>Luteinising hormone</topic><topic>Luteinizing Hormone - blood</topic><topic>Luteinizing Hormone - genetics</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Male</topic><topic>Prolactin</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hotakainen, P.Kristina</creatorcontrib><creatorcontrib>Serlachius, E.Martina</creatorcontrib><creatorcontrib>Lintula, Susanna I.</creatorcontrib><creatorcontrib>Alfthan, Henrik V.</creatorcontrib><creatorcontrib>Schröder, Jim P.</creatorcontrib><creatorcontrib>Stenman, Ulf-Håkan E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hotakainen, P.Kristina</au><au>Serlachius, E.Martina</au><au>Lintula, Susanna I.</au><au>Alfthan, Henrik V.</au><au>Schröder, Jim P.</au><au>Stenman, Ulf-Håkan E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2000-04-25</date><risdate>2000</risdate><volume>162</volume><issue>1</issue><spage>79</spage><epage>85</epage><pages>79-85</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHβ) and chorionic gonadotropin (CGβ) and their translation into protein in various leukocyte subsets. Monocytes, granulocytes, B and T-cells from peripheral blood were separated. Lymphocytes were stimulated with various mitogens, prolactin and mixed lymphocyte culture. LHβ and CGβ mRNA expression was determined by reverse transcriptase polymerase chain reaction. LH, LHβ, CG and CGβ protein were determined in the culture medium by immunofluorometric assays. LHβ mRNA expression was detected in all cell fractions and cultures and stimulation with prolactin induced LH protein in the culture medium. CGβ mRNA expression appeared after culture of lymphocytes, but mitogens and prolactin had no clear stimulating effect. 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| subjects | Adult Base Sequence Cell Line Chorionic gonadotropin Chorionic Gonadotropin, beta Subunit, Human - blood Chorionic Gonadotropin, beta Subunit, Human - genetics Chorionic Gonadotropin, beta Subunit, Human - metabolism DNA Primers - genetics Female Gene Expression Humans In Vitro Techniques Leukocytes Leukocytes - metabolism Luteinising hormone Luteinizing Hormone - blood Luteinizing Hormone - genetics Luteinizing Hormone - metabolism Male Prolactin RNA, Messenger - blood RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes |
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