Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus
Extensive studies have established that light regulates c- fos gene expression in the suprachiasmatic nucleus, the site of an endogenous circadian clock, but relatively little is known about the expression of genes structurally related to c- fos, including fra-1, fra-2 and fosB. We analysed the phot...
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| creator | Schwartz, W.J. Carpino, A. de la Iglesia, H.O. Baler, R. Klein, D.C. Nakabeppu, Y. Aronin, N. |
| description | Extensive studies have established that light regulates c-
fos gene expression in the suprachiasmatic nucleus, the site of an endogenous circadian clock, but relatively little is known about the expression of genes structurally related to c-
fos, including
fra-1,
fra-2 and
fosB. We analysed the photic and temporal regulation of these genes at the messenger RNA and immunoreactive protein levels in rat suprachiasmatic nucleus, and we found different expression patterns after photic stimulation and depending on location in the ventrolateral or dorsomedial subdivisions. In the ventrolateral suprachiasmatic nucleus, c-
fos,
fra-2 and
fosB expression was stimulated after a subjective-night (but not subjective-day) light pulse. Expression of the
fra-2 gene was prolonged following photic stimulation, with elevated messenger RNA and protein levels that appeared unchanged for at least a few hours beyond the c-
fos peak. Unlike c-
fos and
fra-2, the
fosB gene appeared to be expressed constitutively in the ventrolateral suprachiasmatic nucleus throughout the circadian cycle; immunohistochemical analysis suggested that ΔFosB was the protein product accounting for this constitutive expression, while FosB was induced by the subjective-night light pulse. In the dorsomedial suprachiasmatic nucleus, c-
fos and
fra-2 expression exhibited an endogenous circadian rhythm, with higher levels during the early subjective day, although the relative abundance was much lower than that measured after light pulses in the ventrolateral suprachiasmatic nucleus. Double-label immunohistochemistry suggested that some of the dorsomedial cells responsible for the circadian expression of c-Fos also synthesized arginine vasopressin. No evidence of suprachiasmatic nucleus
fra-1 expression was found.
In summary,
fos family genes exhibit differences in their specific expression patterns in the suprachiasmatic nucleus, including their photic and circadian regulation in separate cell populations in the ventrolateral and dorsomedial subdivisions. The data, in combination with our previous results [Takeuchi J.
et al. (1993)
Neuron
11, 825–836], suggest that activator protein-1 binding sites on ventrolateral suprachiasmatic nucleus target genes are constitutively occupied by ΔFosB/JunD complexes, and that c-Fos, Fra-2, FosB and JunB compete for binding after photic stimulation. The differential regulation of
fos family genes in the ventrolateral and dorsomedial suprachiasmatic nucleus suggests that the |
| doi_str_mv | 10.1016/S0306-4522(00)00140-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71219293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0306452200001408</els_id><sourcerecordid>71219293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-ec555a581e2001cce6387ae16d57a3adb413951d448a2f264b3b122febef15f03</originalsourceid><addsrcrecordid>eNqFkE1v1DAQhi1ERZfCTwD5gBAcAv5MsqcKlY8iVeIAnK2JM26NknjxJCv1xk_H6a6gt_piyXred8YPYy-keCeFrN9_F1rUlbFKvRHirRDSiKp9xDaybXTVWGMes80_5JQ9JfolyrFGP2GnUrT1tjXNhv35GEPAjNMcYeAZr5cB5pgmngIPiXiAMQ63_BonJB4nPt8g3xc6p8JhLhmYet6nTGnEfu2gpevjPlIpobVlTWSYy_sug7-JQGOZ4Pm0-AEXesZOAgyEz4_3Gfv5-dOPi8vq6tuXrxcfripvbD1X6K21YFuJqvzVe6x12wDKurcNaOg7I_XWyt6YFlRQtel0J5UK2GGQNgh9xl4fenc5_V6QZjdG8jgMMGFayDVSya3a6gLaA-hzIsoY3C7HEfKtk8Kt6t2derd6dUK4O_WuLbmXxwFLV1TcSx1cF-DVEQDyMIQMk4_0nyurCysLdn7AsNjYR8yOfMTJF7sZ_ez6FB_Y5C8OmKLF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71219293</pqid></control><display><type>article</type><title>Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Schwartz, W.J. ; Carpino, A. ; de la Iglesia, H.O. ; Baler, R. ; Klein, D.C. ; Nakabeppu, Y. ; Aronin, N.</creator><creatorcontrib>Schwartz, W.J. ; Carpino, A. ; de la Iglesia, H.O. ; Baler, R. ; Klein, D.C. ; Nakabeppu, Y. ; Aronin, N.</creatorcontrib><description>Extensive studies have established that light regulates c-
fos gene expression in the suprachiasmatic nucleus, the site of an endogenous circadian clock, but relatively little is known about the expression of genes structurally related to c-
fos, including
fra-1,
fra-2 and
fosB. We analysed the photic and temporal regulation of these genes at the messenger RNA and immunoreactive protein levels in rat suprachiasmatic nucleus, and we found different expression patterns after photic stimulation and depending on location in the ventrolateral or dorsomedial subdivisions. In the ventrolateral suprachiasmatic nucleus, c-
fos,
fra-2 and
fosB expression was stimulated after a subjective-night (but not subjective-day) light pulse. Expression of the
fra-2 gene was prolonged following photic stimulation, with elevated messenger RNA and protein levels that appeared unchanged for at least a few hours beyond the c-
fos peak. Unlike c-
fos and
fra-2, the
fosB gene appeared to be expressed constitutively in the ventrolateral suprachiasmatic nucleus throughout the circadian cycle; immunohistochemical analysis suggested that ΔFosB was the protein product accounting for this constitutive expression, while FosB was induced by the subjective-night light pulse. In the dorsomedial suprachiasmatic nucleus, c-
fos and
fra-2 expression exhibited an endogenous circadian rhythm, with higher levels during the early subjective day, although the relative abundance was much lower than that measured after light pulses in the ventrolateral suprachiasmatic nucleus. Double-label immunohistochemistry suggested that some of the dorsomedial cells responsible for the circadian expression of c-Fos also synthesized arginine vasopressin. No evidence of suprachiasmatic nucleus
fra-1 expression was found.
In summary,
fos family genes exhibit differences in their specific expression patterns in the suprachiasmatic nucleus, including their photic and circadian regulation in separate cell populations in the ventrolateral and dorsomedial subdivisions. The data, in combination with our previous results [Takeuchi J.
et al. (1993)
Neuron
11, 825–836], suggest that activator protein-1 binding sites on ventrolateral suprachiasmatic nucleus target genes are constitutively occupied by ΔFosB/JunD complexes, and that c-Fos, Fra-2, FosB and JunB compete for binding after photic stimulation. The differential regulation of
fos family genes in the ventrolateral and dorsomedial suprachiasmatic nucleus suggests that their circadian function(s) and downstream target(s) are likely to be cell specific.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/S0306-4522(00)00140-8</identifier><identifier>PMID: 10869847</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; AP-1 ; Arginine Vasopressin - genetics ; Biochemistry and metabolism ; Biological and medical sciences ; Central nervous system ; Circadian Rhythm - genetics ; circadian rhythms ; fos ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - physiology ; Genes, Immediate-Early - physiology ; immediate-early genes ; Male ; Photic Stimulation ; Proto-Oncogene Proteins c-fos - genetics ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - analysis ; suprachiasmatic nucleus ; Suprachiasmatic Nucleus - chemistry ; Suprachiasmatic Nucleus - physiology ; vasopressin ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 2000-01, Vol.98 (3), p.535-547</ispartof><rights>2000 IBRO</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ec555a581e2001cce6387ae16d57a3adb413951d448a2f264b3b122febef15f03</citedby><cites>FETCH-LOGICAL-c456t-ec555a581e2001cce6387ae16d57a3adb413951d448a2f264b3b122febef15f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0306-4522(00)00140-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1448051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10869847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schwartz, W.J.</creatorcontrib><creatorcontrib>Carpino, A.</creatorcontrib><creatorcontrib>de la Iglesia, H.O.</creatorcontrib><creatorcontrib>Baler, R.</creatorcontrib><creatorcontrib>Klein, D.C.</creatorcontrib><creatorcontrib>Nakabeppu, Y.</creatorcontrib><creatorcontrib>Aronin, N.</creatorcontrib><title>Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Extensive studies have established that light regulates c-
fos gene expression in the suprachiasmatic nucleus, the site of an endogenous circadian clock, but relatively little is known about the expression of genes structurally related to c-
fos, including
fra-1,
fra-2 and
fosB. We analysed the photic and temporal regulation of these genes at the messenger RNA and immunoreactive protein levels in rat suprachiasmatic nucleus, and we found different expression patterns after photic stimulation and depending on location in the ventrolateral or dorsomedial subdivisions. In the ventrolateral suprachiasmatic nucleus, c-
fos,
fra-2 and
fosB expression was stimulated after a subjective-night (but not subjective-day) light pulse. Expression of the
fra-2 gene was prolonged following photic stimulation, with elevated messenger RNA and protein levels that appeared unchanged for at least a few hours beyond the c-
fos peak. Unlike c-
fos and
fra-2, the
fosB gene appeared to be expressed constitutively in the ventrolateral suprachiasmatic nucleus throughout the circadian cycle; immunohistochemical analysis suggested that ΔFosB was the protein product accounting for this constitutive expression, while FosB was induced by the subjective-night light pulse. In the dorsomedial suprachiasmatic nucleus, c-
fos and
fra-2 expression exhibited an endogenous circadian rhythm, with higher levels during the early subjective day, although the relative abundance was much lower than that measured after light pulses in the ventrolateral suprachiasmatic nucleus. Double-label immunohistochemistry suggested that some of the dorsomedial cells responsible for the circadian expression of c-Fos also synthesized arginine vasopressin. No evidence of suprachiasmatic nucleus
fra-1 expression was found.
In summary,
fos family genes exhibit differences in their specific expression patterns in the suprachiasmatic nucleus, including their photic and circadian regulation in separate cell populations in the ventrolateral and dorsomedial subdivisions. The data, in combination with our previous results [Takeuchi J.
et al. (1993)
Neuron
11, 825–836], suggest that activator protein-1 binding sites on ventrolateral suprachiasmatic nucleus target genes are constitutively occupied by ΔFosB/JunD complexes, and that c-Fos, Fra-2, FosB and JunB compete for binding after photic stimulation. The differential regulation of
fos family genes in the ventrolateral and dorsomedial suprachiasmatic nucleus suggests that their circadian function(s) and downstream target(s) are likely to be cell specific.</description><subject>Animals</subject><subject>AP-1</subject><subject>Arginine Vasopressin - genetics</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Circadian Rhythm - genetics</subject><subject>circadian rhythms</subject><subject>fos</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes, Immediate-Early - physiology</subject><subject>immediate-early genes</subject><subject>Male</subject><subject>Photic Stimulation</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - analysis</subject><subject>suprachiasmatic nucleus</subject><subject>Suprachiasmatic Nucleus - chemistry</subject><subject>Suprachiasmatic Nucleus - physiology</subject><subject>vasopressin</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi1ERZfCTwD5gBAcAv5MsqcKlY8iVeIAnK2JM26NknjxJCv1xk_H6a6gt_piyXred8YPYy-keCeFrN9_F1rUlbFKvRHirRDSiKp9xDaybXTVWGMes80_5JQ9JfolyrFGP2GnUrT1tjXNhv35GEPAjNMcYeAZr5cB5pgmngIPiXiAMQ63_BonJB4nPt8g3xc6p8JhLhmYet6nTGnEfu2gpevjPlIpobVlTWSYy_sug7-JQGOZ4Pm0-AEXesZOAgyEz4_3Gfv5-dOPi8vq6tuXrxcfripvbD1X6K21YFuJqvzVe6x12wDKurcNaOg7I_XWyt6YFlRQtel0J5UK2GGQNgh9xl4fenc5_V6QZjdG8jgMMGFayDVSya3a6gLaA-hzIsoY3C7HEfKtk8Kt6t2derd6dUK4O_WuLbmXxwFLV1TcSx1cF-DVEQDyMIQMk4_0nyurCysLdn7AsNjYR8yOfMTJF7sZ_ez6FB_Y5C8OmKLF</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Schwartz, W.J.</creator><creator>Carpino, A.</creator><creator>de la Iglesia, H.O.</creator><creator>Baler, R.</creator><creator>Klein, D.C.</creator><creator>Nakabeppu, Y.</creator><creator>Aronin, N.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus</title><author>Schwartz, W.J. ; Carpino, A. ; de la Iglesia, H.O. ; Baler, R. ; Klein, D.C. ; Nakabeppu, Y. ; Aronin, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ec555a581e2001cce6387ae16d57a3adb413951d448a2f264b3b122febef15f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>AP-1</topic><topic>Arginine Vasopressin - genetics</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Circadian Rhythm - genetics</topic><topic>circadian rhythms</topic><topic>fos</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genes, Immediate-Early - physiology</topic><topic>immediate-early genes</topic><topic>Male</topic><topic>Photic Stimulation</topic><topic>Proto-Oncogene Proteins c-fos - genetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - analysis</topic><topic>suprachiasmatic nucleus</topic><topic>Suprachiasmatic Nucleus - chemistry</topic><topic>Suprachiasmatic Nucleus - physiology</topic><topic>vasopressin</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwartz, W.J.</creatorcontrib><creatorcontrib>Carpino, A.</creatorcontrib><creatorcontrib>de la Iglesia, H.O.</creatorcontrib><creatorcontrib>Baler, R.</creatorcontrib><creatorcontrib>Klein, D.C.</creatorcontrib><creatorcontrib>Nakabeppu, Y.</creatorcontrib><creatorcontrib>Aronin, N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwartz, W.J.</au><au>Carpino, A.</au><au>de la Iglesia, H.O.</au><au>Baler, R.</au><au>Klein, D.C.</au><au>Nakabeppu, Y.</au><au>Aronin, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>98</volume><issue>3</issue><spage>535</spage><epage>547</epage><pages>535-547</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Extensive studies have established that light regulates c-
fos gene expression in the suprachiasmatic nucleus, the site of an endogenous circadian clock, but relatively little is known about the expression of genes structurally related to c-
fos, including
fra-1,
fra-2 and
fosB. We analysed the photic and temporal regulation of these genes at the messenger RNA and immunoreactive protein levels in rat suprachiasmatic nucleus, and we found different expression patterns after photic stimulation and depending on location in the ventrolateral or dorsomedial subdivisions. In the ventrolateral suprachiasmatic nucleus, c-
fos,
fra-2 and
fosB expression was stimulated after a subjective-night (but not subjective-day) light pulse. Expression of the
fra-2 gene was prolonged following photic stimulation, with elevated messenger RNA and protein levels that appeared unchanged for at least a few hours beyond the c-
fos peak. Unlike c-
fos and
fra-2, the
fosB gene appeared to be expressed constitutively in the ventrolateral suprachiasmatic nucleus throughout the circadian cycle; immunohistochemical analysis suggested that ΔFosB was the protein product accounting for this constitutive expression, while FosB was induced by the subjective-night light pulse. In the dorsomedial suprachiasmatic nucleus, c-
fos and
fra-2 expression exhibited an endogenous circadian rhythm, with higher levels during the early subjective day, although the relative abundance was much lower than that measured after light pulses in the ventrolateral suprachiasmatic nucleus. Double-label immunohistochemistry suggested that some of the dorsomedial cells responsible for the circadian expression of c-Fos also synthesized arginine vasopressin. No evidence of suprachiasmatic nucleus
fra-1 expression was found.
In summary,
fos family genes exhibit differences in their specific expression patterns in the suprachiasmatic nucleus, including their photic and circadian regulation in separate cell populations in the ventrolateral and dorsomedial subdivisions. The data, in combination with our previous results [Takeuchi J.
et al. (1993)
Neuron
11, 825–836], suggest that activator protein-1 binding sites on ventrolateral suprachiasmatic nucleus target genes are constitutively occupied by ΔFosB/JunD complexes, and that c-Fos, Fra-2, FosB and JunB compete for binding after photic stimulation. The differential regulation of
fos family genes in the ventrolateral and dorsomedial suprachiasmatic nucleus suggests that their circadian function(s) and downstream target(s) are likely to be cell specific.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10869847</pmid><doi>10.1016/S0306-4522(00)00140-8</doi><tpages>13</tpages></addata></record> |
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| ispartof | Neuroscience, 2000-01, Vol.98 (3), p.535-547 |
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| subjects | Animals AP-1 Arginine Vasopressin - genetics Biochemistry and metabolism Biological and medical sciences Central nervous system Circadian Rhythm - genetics circadian rhythms fos Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology Genes, Immediate-Early - physiology immediate-early genes Male Photic Stimulation Proto-Oncogene Proteins c-fos - genetics Rats Rats, Sprague-Dawley RNA, Messenger - analysis suprachiasmatic nucleus Suprachiasmatic Nucleus - chemistry Suprachiasmatic Nucleus - physiology vasopressin Vertebrates: nervous system and sense organs |
| title | Differential regulation of fos family genes in the ventrolateral and dorsomedial subdivisions of the rat suprachiasmatic nucleus |
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