MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H
We cloned MafG-2, a novel splice variant of MafG, from rat brain by RT-PCR method. MafG-2 differs from the previously published MafG by an insertion of 27 amino acids. Sequence analysis of the cDNA-encoded MafG-2 showed that MafG-2 contains basic domain and basic leucine zipper (bZip) motif. Transie...
Gespeichert in:
| Veröffentlicht in: | Cellular signalling 2001-11, Vol.13 (11), p.835-839 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 839 |
|---|---|
| container_issue | 11 |
| container_start_page | 835 |
| container_title | Cellular signalling |
| container_volume | 13 |
| creator | Shimokawa, Noriaki Kumaki, Iku Takayama, Kiyoshige |
| description | We cloned MafG-2, a novel splice variant of MafG, from rat brain by RT-PCR method. MafG-2 differs from the previously published MafG by an insertion of 27 amino acids. Sequence analysis of the cDNA-encoded MafG-2 showed that MafG-2 contains basic domain and basic leucine zipper (bZip) motif. Transient transfection studies with GFP-MafG-2 chimera protein indicate that MafG-2 is localized in the nuclei of transfected COS-7 cells. To determine whether gene expression of mafG-2 mRNA is induced by an increase in extracellular protons, we analyzed expression of the mRNA in PC12 cells after an increase in extracellular proton concentration. We found that the mafG-2 mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH value and the expression of mafG-2 mRNA. These results suggest that an increase in extracellular proton may induce the expression of mafG-2 mRNA and MafG-2 may be involved in signal transduction of extracellular of H
+. |
| doi_str_mv | 10.1016/S0898-6568(01)00213-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71214937</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0898656801002133</els_id><sourcerecordid>71214937</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-8a5b0c8e8c29b374629415afa8ce0b06fb5c1b14378c3971cf65c523116469363</originalsourceid><addsrcrecordid>eNqFkEtLAzEQgIMotj5-gpKT6GE1s9lksyeR4gsqIuo5ZNNZjGx3a5IW--9NH-jR04SZbx75CDkBdgkM5NUrU5XKpJDqnMEFYznwjO-QIagyPSrgu2T4iwzIQQifjIFgMt8nAwChElMNycuTae6znLpADe36BbY0ZejM9xFdR-OHiasafs88hoATWi9piG46b010fUf7JtWiNxbbNuU8fTgie41pAx5v4yF5v7t9Gz1k4-f7x9HNOLNcQsyUETWzCpXNq5qXhcyrAoRpjLLIaiabWliooeClsrwqwTZSWJFzAFnIikt-SM42c9OtX3MMUU9dWJ1hOuznQZeQQ1HxMoFiA1rfh-Cx0TPvpsYvNTC9cqnXLvVKlGag1y41T32n2wXzeoqTv66tvARcbwBM31w49DpYh53FifNoo5707p8VP0Dqgh0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71214937</pqid></control><display><type>article</type><title>MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Shimokawa, Noriaki ; Kumaki, Iku ; Takayama, Kiyoshige</creator><creatorcontrib>Shimokawa, Noriaki ; Kumaki, Iku ; Takayama, Kiyoshige</creatorcontrib><description>We cloned MafG-2, a novel splice variant of MafG, from rat brain by RT-PCR method. MafG-2 differs from the previously published MafG by an insertion of 27 amino acids. Sequence analysis of the cDNA-encoded MafG-2 showed that MafG-2 contains basic domain and basic leucine zipper (bZip) motif. Transient transfection studies with GFP-MafG-2 chimera protein indicate that MafG-2 is localized in the nuclei of transfected COS-7 cells. To determine whether gene expression of mafG-2 mRNA is induced by an increase in extracellular protons, we analyzed expression of the mRNA in PC12 cells after an increase in extracellular proton concentration. We found that the mafG-2 mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH value and the expression of mafG-2 mRNA. These results suggest that an increase in extracellular proton may induce the expression of mafG-2 mRNA and MafG-2 may be involved in signal transduction of extracellular of H
+.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/S0898-6568(01)00213-3</identifier><identifier>PMID: 11583919</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Chimera protein ; Cloning, Molecular ; COS Cells ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - genetics ; Extracellular H ; Extracellular Space - physiology ; Hydrogen-Ion Concentration ; MafG Transcription Factor ; MafG-2 ; Mice ; Molecular Sequence Data ; Nuclear Proteins - biosynthesis ; Nuclear Proteins - genetics ; Nuclear transcription factor ; PC12 Cells ; Phylogeny ; Protons ; Rats ; Repressor Proteins - biosynthesis ; Repressor Proteins - genetics ; RNA, Messenger - biosynthesis ; Sequence Homology, Amino Acid ; Small Maf protein family ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcriptional Activation</subject><ispartof>Cellular signalling, 2001-11, Vol.13 (11), p.835-839</ispartof><rights>2001 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-8a5b0c8e8c29b374629415afa8ce0b06fb5c1b14378c3971cf65c523116469363</citedby><cites>FETCH-LOGICAL-c361t-8a5b0c8e8c29b374629415afa8ce0b06fb5c1b14378c3971cf65c523116469363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0898656801002133$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11583919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimokawa, Noriaki</creatorcontrib><creatorcontrib>Kumaki, Iku</creatorcontrib><creatorcontrib>Takayama, Kiyoshige</creatorcontrib><title>MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>We cloned MafG-2, a novel splice variant of MafG, from rat brain by RT-PCR method. MafG-2 differs from the previously published MafG by an insertion of 27 amino acids. Sequence analysis of the cDNA-encoded MafG-2 showed that MafG-2 contains basic domain and basic leucine zipper (bZip) motif. Transient transfection studies with GFP-MafG-2 chimera protein indicate that MafG-2 is localized in the nuclei of transfected COS-7 cells. To determine whether gene expression of mafG-2 mRNA is induced by an increase in extracellular protons, we analyzed expression of the mRNA in PC12 cells after an increase in extracellular proton concentration. We found that the mafG-2 mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH value and the expression of mafG-2 mRNA. These results suggest that an increase in extracellular proton may induce the expression of mafG-2 mRNA and MafG-2 may be involved in signal transduction of extracellular of H
+.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Chimera protein</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Extracellular H</subject><subject>Extracellular Space - physiology</subject><subject>Hydrogen-Ion Concentration</subject><subject>MafG Transcription Factor</subject><subject>MafG-2</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear transcription factor</subject><subject>PC12 Cells</subject><subject>Phylogeny</subject><subject>Protons</subject><subject>Rats</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sequence Homology, Amino Acid</subject><subject>Small Maf protein family</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcriptional Activation</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgIMotj5-gpKT6GE1s9lksyeR4gsqIuo5ZNNZjGx3a5IW--9NH-jR04SZbx75CDkBdgkM5NUrU5XKpJDqnMEFYznwjO-QIagyPSrgu2T4iwzIQQifjIFgMt8nAwChElMNycuTae6znLpADe36BbY0ZejM9xFdR-OHiasafs88hoATWi9piG46b010fUf7JtWiNxbbNuU8fTgie41pAx5v4yF5v7t9Gz1k4-f7x9HNOLNcQsyUETWzCpXNq5qXhcyrAoRpjLLIaiabWliooeClsrwqwTZSWJFzAFnIikt-SM42c9OtX3MMUU9dWJ1hOuznQZeQQ1HxMoFiA1rfh-Cx0TPvpsYvNTC9cqnXLvVKlGag1y41T32n2wXzeoqTv66tvARcbwBM31w49DpYh53FifNoo5707p8VP0Dqgh0</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Shimokawa, Noriaki</creator><creator>Kumaki, Iku</creator><creator>Takayama, Kiyoshige</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H</title><author>Shimokawa, Noriaki ; Kumaki, Iku ; Takayama, Kiyoshige</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-8a5b0c8e8c29b374629415afa8ce0b06fb5c1b14378c3971cf65c523116469363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Chimera protein</topic><topic>Cloning, Molecular</topic><topic>COS Cells</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Extracellular H</topic><topic>Extracellular Space - physiology</topic><topic>Hydrogen-Ion Concentration</topic><topic>MafG Transcription Factor</topic><topic>MafG-2</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear transcription factor</topic><topic>PC12 Cells</topic><topic>Phylogeny</topic><topic>Protons</topic><topic>Rats</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sequence Homology, Amino Acid</topic><topic>Small Maf protein family</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimokawa, Noriaki</creatorcontrib><creatorcontrib>Kumaki, Iku</creatorcontrib><creatorcontrib>Takayama, Kiyoshige</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimokawa, Noriaki</au><au>Kumaki, Iku</au><au>Takayama, Kiyoshige</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>13</volume><issue>11</issue><spage>835</spage><epage>839</epage><pages>835-839</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>We cloned MafG-2, a novel splice variant of MafG, from rat brain by RT-PCR method. MafG-2 differs from the previously published MafG by an insertion of 27 amino acids. Sequence analysis of the cDNA-encoded MafG-2 showed that MafG-2 contains basic domain and basic leucine zipper (bZip) motif. Transient transfection studies with GFP-MafG-2 chimera protein indicate that MafG-2 is localized in the nuclei of transfected COS-7 cells. To determine whether gene expression of mafG-2 mRNA is induced by an increase in extracellular protons, we analyzed expression of the mRNA in PC12 cells after an increase in extracellular proton concentration. We found that the mafG-2 mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH value and the expression of mafG-2 mRNA. These results suggest that an increase in extracellular proton may induce the expression of mafG-2 mRNA and MafG-2 may be involved in signal transduction of extracellular of H
+.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>11583919</pmid><doi>10.1016/S0898-6568(01)00213-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0898-6568 |
| ispartof | Cellular signalling, 2001-11, Vol.13 (11), p.835-839 |
| issn | 0898-6568 1873-3913 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71214937 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Chimera protein Cloning, Molecular COS Cells DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics Extracellular H Extracellular Space - physiology Hydrogen-Ion Concentration MafG Transcription Factor MafG-2 Mice Molecular Sequence Data Nuclear Proteins - biosynthesis Nuclear Proteins - genetics Nuclear transcription factor PC12 Cells Phylogeny Protons Rats Repressor Proteins - biosynthesis Repressor Proteins - genetics RNA, Messenger - biosynthesis Sequence Homology, Amino Acid Small Maf protein family Transcription Factors - biosynthesis Transcription Factors - genetics Transcriptional Activation |
| title | MafG-2 is a novel Maf protein that is expressed by stimulation of extracellular H |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T15%3A45%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MafG-2%20is%20a%20novel%20Maf%20protein%20that%20is%20expressed%20by%20stimulation%20of%20extracellular%20H&rft.jtitle=Cellular%20signalling&rft.au=Shimokawa,%20Noriaki&rft.date=2001-11-01&rft.volume=13&rft.issue=11&rft.spage=835&rft.epage=839&rft.pages=835-839&rft.issn=0898-6568&rft.eissn=1873-3913&rft_id=info:doi/10.1016/S0898-6568(01)00213-3&rft_dat=%3Cproquest_cross%3E71214937%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71214937&rft_id=info:pmid/11583919&rft_els_id=S0898656801002133&rfr_iscdi=true |