Identification of Novel Membrane and Secreted Proteins Upregulated during Adipocyte Differentiation
Adipose tissue is the largest organ in the body that secretes soluble proteins such as cytokines. A preadipocyte cell line 3T3-L1 has been widely used for investigations of mechanisms of adipocyte differentiation. 3T3-L1 cells convert to adipocytes in the presence of 1-methyl-3-isobutylxanthine, dex...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-05, Vol.272 (1), p.293-297 |
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| creator | Tsuruga, Hiromichi Kumagai, Hidetoshi Kojima, Tetsuo Kitamura, Toshio |
| description | Adipose tissue is the largest organ in the body that secretes soluble proteins such as cytokines. A preadipocyte cell line 3T3-L1 has been widely used for investigations of mechanisms of adipocyte differentiation. 3T3-L1 cells convert to adipocytes in the presence of 1-methyl-3-isobutylxanthine, dexamethasone, and insulin. We screened a cDNA library derived from differentiated 3T3-L1 cells, using the SST-REX method (signal sequence trap by retrovirus-mediated expression screening method). Screening of 4 × 105 clones gave rise to 63 known and 8 novel clones. The known clones represented 28 independent proteins, 21 of which were secreted proteins and 7 were membrane proteins. The novel clones represented 7 independent proteins, 5 of which had no similarity to known proteins. Interestingly, most of these novel genes showed differentiation- and tissue-specific expression. The present results indicate that adipocytes specific genes or adipocyte differentiation-related genes encoding membrane and secreted proteins can be readily identified if signal sequence trap screening of differentiated adipocyte-derived cDNAs is done. |
| doi_str_mv | 10.1006/bbrc.2000.2759 |
| format | Article |
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A preadipocyte cell line 3T3-L1 has been widely used for investigations of mechanisms of adipocyte differentiation. 3T3-L1 cells convert to adipocytes in the presence of 1-methyl-3-isobutylxanthine, dexamethasone, and insulin. We screened a cDNA library derived from differentiated 3T3-L1 cells, using the SST-REX method (signal sequence trap by retrovirus-mediated expression screening method). Screening of 4 × 105 clones gave rise to 63 known and 8 novel clones. The known clones represented 28 independent proteins, 21 of which were secreted proteins and 7 were membrane proteins. The novel clones represented 7 independent proteins, 5 of which had no similarity to known proteins. Interestingly, most of these novel genes showed differentiation- and tissue-specific expression. 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| subjects | 3T3 Cells 3T3-L1 adipocyte Adipocytes - cytology Adipocytes - metabolism Animals Cell Differentiation differentiation DNA, Complementary - genetics Gene Expression Male Membrane Proteins - genetics Membrane Proteins - metabolism Mice Proteins - genetics Proteins - metabolism Proteins - secretion retrovirus cDNA library RNA, Messenger - genetics RNA, Messenger - metabolism signal sequence trap Tissue Distribution Up-Regulation |
| title | Identification of Novel Membrane and Secreted Proteins Upregulated during Adipocyte Differentiation |
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