Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus
The involvement of apoptosis in the lymphatic organs of piglets infected with classical swine fever (CSF) virus was investigated. Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages...
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| Veröffentlicht in: | Veterinary microbiology 2000-07, Vol.75 (1), p.1-9 |
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| creator | Sato, M Mikami, O Kobayashi, M Nakajima, Y |
| description | The involvement of apoptosis in the lymphatic organs of piglets infected with classical swine fever (CSF) virus was investigated. Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages phagocytizing the nuclear remnants or apoptotic bodies increased after 3 DPI. Thymus atrophy due to the loss of the cortex increased markedly during the observation period. Compact and shrunken nuclei indicating apoptosis were observed in the spleen and lymph node. DNA fragmentation was detected in the nuclei of lymphocytes in the thymus, spleen and lymph nodes, as well as at sites of focal necrosis. Using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, ultrastructural characteristics of apoptosis, i.e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF. |
| doi_str_mv | 10.1016/S0378-1135(00)00198-X |
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Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages phagocytizing the nuclear remnants or apoptotic bodies increased after 3 DPI. Thymus atrophy due to the loss of the cortex increased markedly during the observation period. Compact and shrunken nuclei indicating apoptosis were observed in the spleen and lymph node. DNA fragmentation was detected in the nuclei of lymphocytes in the thymus, spleen and lymph nodes, as well as at sites of focal necrosis. Using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, ultrastructural characteristics of apoptosis, i.e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(00)00198-X</identifier><identifier>PMID: 10865147</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Classical swine fever (CSF) ; Classical Swine Fever - pathology ; Classical Swine Fever - virology ; Classical Swine Fever Virus - pathogenicity ; Experimental viral diseases and models ; Female ; Histocytochemistry ; In Situ Nick-End Labeling - veterinary ; Infectious diseases ; Lymph Nodes - pathology ; Lymph Nodes - virology ; Lymphatic organ ; Lymphoid Tissue - pathology ; Lymphoid Tissue - virology ; Male ; Medical sciences ; Microscopy, Electron - veterinary ; Piglet ; pigs ; Spleen - pathology ; Spleen - virology ; Swine ; Swine fever virus ; Thymus Gland - pathology ; Thymus Gland - virology ; Viral diseases</subject><ispartof>Veterinary microbiology, 2000-07, Vol.75 (1), p.1-9</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-bbc9c37862f6ab8aeca050e9c06f7bc178a1fd21c41de1ea5d1c937405c1fac93</citedby><cites>FETCH-LOGICAL-c420t-bbc9c37862f6ab8aeca050e9c06f7bc178a1fd21c41de1ea5d1c937405c1fac93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(00)00198-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=833862$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10865147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sato, M</creatorcontrib><creatorcontrib>Mikami, O</creatorcontrib><creatorcontrib>Kobayashi, M</creatorcontrib><creatorcontrib>Nakajima, Y</creatorcontrib><title>Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The involvement of apoptosis in the lymphatic organs of piglets infected with classical swine fever (CSF) virus was investigated. Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages phagocytizing the nuclear remnants or apoptotic bodies increased after 3 DPI. Thymus atrophy due to the loss of the cortex increased markedly during the observation period. Compact and shrunken nuclei indicating apoptosis were observed in the spleen and lymph node. DNA fragmentation was detected in the nuclei of lymphocytes in the thymus, spleen and lymph nodes, as well as at sites of focal necrosis. Using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, ultrastructural characteristics of apoptosis, i.e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Classical swine fever (CSF)</subject><subject>Classical Swine Fever - pathology</subject><subject>Classical Swine Fever - virology</subject><subject>Classical Swine Fever Virus - pathogenicity</subject><subject>Experimental viral diseases and models</subject><subject>Female</subject><subject>Histocytochemistry</subject><subject>In Situ Nick-End Labeling - veterinary</subject><subject>Infectious diseases</subject><subject>Lymph Nodes - pathology</subject><subject>Lymph Nodes - virology</subject><subject>Lymphatic organ</subject><subject>Lymphoid Tissue - pathology</subject><subject>Lymphoid Tissue - virology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Electron - veterinary</subject><subject>Piglet</subject><subject>pigs</subject><subject>Spleen - pathology</subject><subject>Spleen - virology</subject><subject>Swine</subject><subject>Swine fever virus</subject><subject>Thymus Gland - pathology</subject><subject>Thymus Gland - virology</subject><subject>Viral diseases</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1r3DAQgGFRGppt2p_QIiiU9uB0xt8-hRD6BYEc2sLehDweZVW8livJG_Lvo80uobfoIh0eScMrxDuEcwSsv_yComkzxKL6BPAZALs2W78QK2ybIsurMn8pVk_kVLwO4S8AlF0Nr8QpQltXWDYrsb6c3RxdsEHaScYNy_F-O290tCSdv9VTkM7I2d6OHPfE0TLqyIO8s3EjadQhWNKjDHd2Yml4x17urF_CG3Fi9Bj47XE_E3--ff199SO7vvn-8-ryOqMyh5j1PXWUxqxzU-u-1UwaKuCOoDZNT9i0Gs2QI5U4MLKuBqSuaEqoCI1OxzPx8fDu7N2_hUNUWxuIx1FP7JagGswRyrx-FmJTpQV7WB0geReCZ6Nmb7fa3ysEtW-vHturfVgFoB7bq3W69_74wdJvefjv1iF2Ah-OQIfUzHg9kQ1Pri2KlCGpi4PiVG1n2atAlifiwXqmqAZnnxnkAUajoeI</recordid><startdate>20000703</startdate><enddate>20000703</enddate><creator>Sato, M</creator><creator>Mikami, O</creator><creator>Kobayashi, M</creator><creator>Nakajima, Y</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000703</creationdate><title>Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus</title><author>Sato, M ; Mikami, O ; Kobayashi, M ; Nakajima, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-bbc9c37862f6ab8aeca050e9c06f7bc178a1fd21c41de1ea5d1c937405c1fac93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Classical swine fever (CSF)</topic><topic>Classical Swine Fever - pathology</topic><topic>Classical Swine Fever - virology</topic><topic>Classical Swine Fever Virus - pathogenicity</topic><topic>Experimental viral diseases and models</topic><topic>Female</topic><topic>Histocytochemistry</topic><topic>In Situ Nick-End Labeling - veterinary</topic><topic>Infectious diseases</topic><topic>Lymph Nodes - pathology</topic><topic>Lymph Nodes - virology</topic><topic>Lymphatic organ</topic><topic>Lymphoid Tissue - pathology</topic><topic>Lymphoid Tissue - virology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Electron - veterinary</topic><topic>Piglet</topic><topic>pigs</topic><topic>Spleen - pathology</topic><topic>Spleen - virology</topic><topic>Swine</topic><topic>Swine fever virus</topic><topic>Thymus Gland - pathology</topic><topic>Thymus Gland - virology</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, M</creatorcontrib><creatorcontrib>Mikami, O</creatorcontrib><creatorcontrib>Kobayashi, M</creatorcontrib><creatorcontrib>Nakajima, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, M</au><au>Mikami, O</au><au>Kobayashi, M</au><au>Nakajima, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2000-07-03</date><risdate>2000</risdate><volume>75</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The involvement of apoptosis in the lymphatic organs of piglets infected with classical swine fever (CSF) virus was investigated. Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages phagocytizing the nuclear remnants or apoptotic bodies increased after 3 DPI. Thymus atrophy due to the loss of the cortex increased markedly during the observation period. Compact and shrunken nuclei indicating apoptosis were observed in the spleen and lymph node. DNA fragmentation was detected in the nuclei of lymphocytes in the thymus, spleen and lymph nodes, as well as at sites of focal necrosis. Using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, ultrastructural characteristics of apoptosis, i.e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10865147</pmid><doi>10.1016/S0378-1135(00)00198-X</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Apoptosis Biological and medical sciences Classical swine fever (CSF) Classical Swine Fever - pathology Classical Swine Fever - virology Classical Swine Fever Virus - pathogenicity Experimental viral diseases and models Female Histocytochemistry In Situ Nick-End Labeling - veterinary Infectious diseases Lymph Nodes - pathology Lymph Nodes - virology Lymphatic organ Lymphoid Tissue - pathology Lymphoid Tissue - virology Male Medical sciences Microscopy, Electron - veterinary Piglet pigs Spleen - pathology Spleen - virology Swine Swine fever virus Thymus Gland - pathology Thymus Gland - virology Viral diseases |
| title | Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus |
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