T Cell-dependent Inducible Nitric Oxide Synthase Production and Ultrastructural Morphology in BALB/c Mice Infected with Mycobacterium avium subspecies paratuberculosis
Euthymic BALB/c and athymic nude BALB/c mice aged 3–8 days were infected intraperitoneally with Mycobacterium avium subspecies paratuberculosis (ATCC strain 19698). After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured fr...
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| Veröffentlicht in: | Journal of comparative pathology 2001-08, Vol.125 (2-3), p.137-144 |
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| creator | Thomsen, B.V. Steadham, E.M. Gallup, J.M. Ackermann, M.R. Brees, D.J. Cheville, N.F. |
| description | Euthymic BALB/c and athymic nude BALB/c mice aged 3–8 days were infected intraperitoneally with Mycobacterium avium subspecies paratuberculosis (ATCC strain 19698). After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured from light microscopy sections, by electron microscopy and by immunohistochemical methods. Euthymic mice differed from athymic mice in that (1) their hepatic granulomas were smaller, contained fewer bacteria, and produced more inducible nitric oxide synthase, and (2) their hepatic macrophages contained fewer bacteria, a higher percentage of degraded bacteria, and increased numbers of primary lysosomes. The study showed that macrophage activation was markedly less in the T cell-deficient athymic mice than in the euthymic mice. |
| doi_str_mv | 10.1053/jcpa.2001.0491 |
| format | Article |
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After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured from light microscopy sections, by electron microscopy and by immunohistochemical methods. Euthymic mice differed from athymic mice in that (1) their hepatic granulomas were smaller, contained fewer bacteria, and produced more inducible nitric oxide synthase, and (2) their hepatic macrophages contained fewer bacteria, a higher percentage of degraded bacteria, and increased numbers of primary lysosomes. The study showed that macrophage activation was markedly less in the T cell-deficient athymic mice than in the euthymic mice.</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1053/jcpa.2001.0491</identifier><identifier>PMID: 11578129</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Animals, Newborn ; Disease Models, Animal ; Fluorescent Antibody Technique, Indirect ; Granuloma - enzymology ; Granuloma - parasitology ; Granuloma - pathology ; Image Processing, Computer-Assisted ; Immunoenzyme Techniques ; Liver - enzymology ; Liver - immunology ; Liver - ultrastructure ; Lysosomes - microbiology ; Lysosomes - ultrastructure ; Macrophages - enzymology ; Macrophages - immunology ; Macrophages - ultrastructure ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Mycobacterium avium subsp. paratuberculosis - physiology ; Nitric Oxide Synthase - biosynthesis ; Nitric Oxide Synthase - immunology ; Nitric Oxide Synthase Type II ; Paratuberculosis - enzymology ; Paratuberculosis - immunology ; Paratuberculosis - pathology ; T-Lymphocytes - immunology</subject><ispartof>Journal of comparative pathology, 2001-08, Vol.125 (2-3), p.137-144</ispartof><rights>2001 Harcourt Publishers Ltd</rights><rights>Copyright Harcourt Publishers Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-4a1b790f9df5bee5c4c5518e43b72912262a2ba3505d0b194e0b26d773ed91303</citedby><cites>FETCH-LOGICAL-c340t-4a1b790f9df5bee5c4c5518e43b72912262a2ba3505d0b194e0b26d773ed91303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021997501904918$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11578129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomsen, B.V.</creatorcontrib><creatorcontrib>Steadham, E.M.</creatorcontrib><creatorcontrib>Gallup, J.M.</creatorcontrib><creatorcontrib>Ackermann, M.R.</creatorcontrib><creatorcontrib>Brees, D.J.</creatorcontrib><creatorcontrib>Cheville, N.F.</creatorcontrib><title>T Cell-dependent Inducible Nitric Oxide Synthase Production and Ultrastructural Morphology in BALB/c Mice Infected with Mycobacterium avium subspecies paratuberculosis</title><title>Journal of comparative pathology</title><addtitle>J Comp Pathol</addtitle><description>Euthymic BALB/c and athymic nude BALB/c mice aged 3–8 days were infected intraperitoneally with Mycobacterium avium subspecies paratuberculosis (ATCC strain 19698). After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured from light microscopy sections, by electron microscopy and by immunohistochemical methods. Euthymic mice differed from athymic mice in that (1) their hepatic granulomas were smaller, contained fewer bacteria, and produced more inducible nitric oxide synthase, and (2) their hepatic macrophages contained fewer bacteria, a higher percentage of degraded bacteria, and increased numbers of primary lysosomes. The study showed that macrophage activation was markedly less in the T cell-deficient athymic mice than in the euthymic mice.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Disease Models, Animal</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Granuloma - enzymology</subject><subject>Granuloma - parasitology</subject><subject>Granuloma - pathology</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunoenzyme Techniques</subject><subject>Liver - enzymology</subject><subject>Liver - immunology</subject><subject>Liver - ultrastructure</subject><subject>Lysosomes - microbiology</subject><subject>Lysosomes - ultrastructure</subject><subject>Macrophages - enzymology</subject><subject>Macrophages - immunology</subject><subject>Macrophages - ultrastructure</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Mycobacterium avium subsp. paratuberculosis - physiology</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Nitric Oxide Synthase - immunology</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Paratuberculosis - enzymology</subject><subject>Paratuberculosis - immunology</subject><subject>Paratuberculosis - pathology</subject><subject>T-Lymphocytes - immunology</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1O3DAURi3UCqa02y4rr7rL4OvEJFnCiLZIMwUJWFv-uekYZeLUdmjniXhNHM1IXXXjK1vnfpK_Q8hnYEtgorx4NqNacsZgyaoWTsgCRMmLEnj7jiwY41C0bS3OyIcYnxljTSP4KTkDEHWTmQV5faQr7PvC4oiDxSHR28FOxuke6U-XgjP07q-zSB_2Q9qqiPQ--Awk5weqBkuf-hRUTCE_TUH1dOPDuPW9_7WnbqDXV-vrC0M3zmAO7tAktPSPS1u62RuvVb4HN-2oepnPOOk4onEY6aiCSpPGYKbeRxc_kved6iN-Os5z8vTt5nH1o1jffb9dXa0LU1YsFZUCXbesa20nNKIwlRECGqxKXfMWOL_kimtVCiYs09BWyDS_tHVdom2hZOU5-XrIHYP_PWFMcueiyQ2pAf0UZQ0cGADP4PIAmuBjDNjJMbidCnsJTM5q5KxGzmrkrCYvfDkmT3qH9h9-dJGB5gBg_t-LwyBjrmIwaF3IxUnr3f-y3wArSqCo</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Thomsen, B.V.</creator><creator>Steadham, E.M.</creator><creator>Gallup, J.M.</creator><creator>Ackermann, M.R.</creator><creator>Brees, D.J.</creator><creator>Cheville, N.F.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>T Cell-dependent Inducible Nitric Oxide Synthase Production and Ultrastructural Morphology in BALB/c Mice Infected with Mycobacterium avium subspecies paratuberculosis</title><author>Thomsen, B.V. ; 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After euthanasia at 5 months post-inoculation, hepatic granulomas were evaluated by morphometric analysis of digital images captured from light microscopy sections, by electron microscopy and by immunohistochemical methods. Euthymic mice differed from athymic mice in that (1) their hepatic granulomas were smaller, contained fewer bacteria, and produced more inducible nitric oxide synthase, and (2) their hepatic macrophages contained fewer bacteria, a higher percentage of degraded bacteria, and increased numbers of primary lysosomes. The study showed that macrophage activation was markedly less in the T cell-deficient athymic mice than in the euthymic mice.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11578129</pmid><doi>10.1053/jcpa.2001.0491</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Disease Models, Animal Fluorescent Antibody Technique, Indirect Granuloma - enzymology Granuloma - parasitology Granuloma - pathology Image Processing, Computer-Assisted Immunoenzyme Techniques Liver - enzymology Liver - immunology Liver - ultrastructure Lysosomes - microbiology Lysosomes - ultrastructure Macrophages - enzymology Macrophages - immunology Macrophages - ultrastructure Mice Mice, Inbred BALB C Mice, Nude Mycobacterium avium subsp. paratuberculosis - physiology Nitric Oxide Synthase - biosynthesis Nitric Oxide Synthase - immunology Nitric Oxide Synthase Type II Paratuberculosis - enzymology Paratuberculosis - immunology Paratuberculosis - pathology T-Lymphocytes - immunology |
| title | T Cell-dependent Inducible Nitric Oxide Synthase Production and Ultrastructural Morphology in BALB/c Mice Infected with Mycobacterium avium subspecies paratuberculosis |
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