Effect of dexamethasone on insulin-like growth factor–1 expression in a rabbit model of growth retardation
Background/Purpose: The maternal administration of steroids promotes fetal maturative effects in the gastrointestinal tract. To determine if fetal insulin-like growth factor–1 (IGF-1) expression is altered in response to maternal dexamethasone administration, this rabbit model of intrauterine growth...
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| Veröffentlicht in: | Journal of pediatric surgery 2000-06, Vol.35 (6), p.898-905 |
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| creator | Thakur, A. Sase, M. Lee, J.J. Thakur, V. Buchmiller, T.L. |
| description | Background/Purpose: The maternal administration of steroids promotes fetal maturative effects in the gastrointestinal tract. To determine if fetal insulin-like growth factor–1 (IGF-1) expression is altered in response to maternal dexamethasone administration, this rabbit model of intrauterine growth retardation (IUGR) was utilized. Methods: Eight pregnant rabbits received either dexamethasone (Dex 0.1 mg/kg/d intramuscular), or normal saline (Cont) on gestational days 26 and 27. Fetuses were harvested on gestational day 28 or 29 and were identified as favored (Fav) or runt (Runt): DexFav, DexRunt, ContFav, and ContRunt. Fetal weight was recorded and the serum, amniotic fluid, liver, kidney, and small intestine (SI) were collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure IGF-1/β-actin mRNA densitometric band ratios in all tissues. Radioimmunoassay (RIA) was used to measure IGF-1 protein levels in the serum and amniotic fluid. Results: Weight was decreased in the Runt fetuses at all time-points (P |
| doi_str_mv | 10.1053/jpsu.2000.6914 |
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To determine if fetal insulin-like growth factor–1 (IGF-1) expression is altered in response to maternal dexamethasone administration, this rabbit model of intrauterine growth retardation (IUGR) was utilized. Methods: Eight pregnant rabbits received either dexamethasone (Dex 0.1 mg/kg/d intramuscular), or normal saline (Cont) on gestational days 26 and 27. Fetuses were harvested on gestational day 28 or 29 and were identified as favored (Fav) or runt (Runt): DexFav, DexRunt, ContFav, and ContRunt. Fetal weight was recorded and the serum, amniotic fluid, liver, kidney, and small intestine (SI) were collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure IGF-1/β-actin mRNA densitometric band ratios in all tissues. Radioimmunoassay (RIA) was used to measure IGF-1 protein levels in the serum and amniotic fluid. Results: Weight was decreased in the Runt fetuses at all time-points (P <.08). The percent weight accretion from day 28 to 29, was greatest in the DexRunt fetus (P <.001), suggesting “catch-up” growth. All Dex fetuses (Fav and Runt) had increased liver and proximal, middle and distal SI IGF-1 mRNA at day 28 and elevated levels in the liver, proximal and distal SI at day 29 compared with control fetuses. The DexRunt fetuses had serum IGF-1 protein surpassing that of the DexFav fetus at day 28. Conclusions: This report provides the first description of maternal steroid administration effecting a marked increase in fetal IGF-1 mRNA expression and IGF-1 protein levels in an in vivo rabbit model of IUGR. The growth-retarded fetus appears to be particularly responsive. J Pediatr Surg 35:898-905. Copyright © 2000 by W.B. Saunders Company.</description><identifier>ISSN: 0022-3468</identifier><identifier>EISSN: 1531-5037</identifier><identifier>DOI: 10.1053/jpsu.2000.6914</identifier><identifier>PMID: 10873033</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actins - metabolism ; Animals ; Body Weight ; dexamethasone ; Dexamethasone - pharmacology ; Female ; Fetal Growth Retardation - metabolism ; fetus ; Fetus - metabolism ; Glucocorticoids - pharmacology ; Insulin-Like Growth Factor I - biosynthesis ; insulin-like growth factor–1 (IGF-1) ; Intrauterine growth retardation (IUGR) ; Pregnancy ; Rabbits ; Reverse Transcriptase Polymerase Chain Reaction ; small intestine</subject><ispartof>Journal of pediatric surgery, 2000-06, Vol.35 (6), p.898-905</ispartof><rights>2000 W.B. Saunders Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-4dd734f83f33573a83dda5e4919d67f304c4cb6f6e9f217401f8d4d7191dd0433</citedby><cites>FETCH-LOGICAL-c340t-4dd734f83f33573a83dda5e4919d67f304c4cb6f6e9f217401f8d4d7191dd0433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022346800297467$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10873033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thakur, A.</creatorcontrib><creatorcontrib>Sase, M.</creatorcontrib><creatorcontrib>Lee, J.J.</creatorcontrib><creatorcontrib>Thakur, V.</creatorcontrib><creatorcontrib>Buchmiller, T.L.</creatorcontrib><title>Effect of dexamethasone on insulin-like growth factor–1 expression in a rabbit model of growth retardation</title><title>Journal of pediatric surgery</title><addtitle>J Pediatr Surg</addtitle><description>Background/Purpose: The maternal administration of steroids promotes fetal maturative effects in the gastrointestinal tract. To determine if fetal insulin-like growth factor–1 (IGF-1) expression is altered in response to maternal dexamethasone administration, this rabbit model of intrauterine growth retardation (IUGR) was utilized. Methods: Eight pregnant rabbits received either dexamethasone (Dex 0.1 mg/kg/d intramuscular), or normal saline (Cont) on gestational days 26 and 27. Fetuses were harvested on gestational day 28 or 29 and were identified as favored (Fav) or runt (Runt): DexFav, DexRunt, ContFav, and ContRunt. Fetal weight was recorded and the serum, amniotic fluid, liver, kidney, and small intestine (SI) were collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure IGF-1/β-actin mRNA densitometric band ratios in all tissues. Radioimmunoassay (RIA) was used to measure IGF-1 protein levels in the serum and amniotic fluid. Results: Weight was decreased in the Runt fetuses at all time-points (P <.08). The percent weight accretion from day 28 to 29, was greatest in the DexRunt fetus (P <.001), suggesting “catch-up” growth. All Dex fetuses (Fav and Runt) had increased liver and proximal, middle and distal SI IGF-1 mRNA at day 28 and elevated levels in the liver, proximal and distal SI at day 29 compared with control fetuses. The DexRunt fetuses had serum IGF-1 protein surpassing that of the DexFav fetus at day 28. Conclusions: This report provides the first description of maternal steroid administration effecting a marked increase in fetal IGF-1 mRNA expression and IGF-1 protein levels in an in vivo rabbit model of IUGR. The growth-retarded fetus appears to be particularly responsive. J Pediatr Surg 35:898-905. Copyright © 2000 by W.B. Saunders Company.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Body Weight</subject><subject>dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>Female</subject><subject>Fetal Growth Retardation - metabolism</subject><subject>fetus</subject><subject>Fetus - metabolism</subject><subject>Glucocorticoids - pharmacology</subject><subject>Insulin-Like Growth Factor I - biosynthesis</subject><subject>insulin-like growth factor–1 (IGF-1)</subject><subject>Intrauterine growth retardation (IUGR)</subject><subject>Pregnancy</subject><subject>Rabbits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>small intestine</subject><issn>0022-3468</issn><issn>1531-5037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLFu1TAUhi0EopfC2hF5YsvlOMeJk7GqWkCqxAKz5WsfU7dJfLGdtmy8Q9-QJyHh3qEL01m-_5POx9iZgK2ABj_e7vO8rQFg2_ZCvmAb0aCoGkD1km0A6rpC2XYn7E3OtwuFCsRrdiKgUwiIGzZcek-28Oi5o0czUrkxOU7E48TDlOchTNUQ7oj_SPGh3HBvbInpz-8nwelxnyjn8I_khiez24XCx-hoWH3HRaJikjNl4d6yV94Mmd4d7yn7fnX57eJzdf3105eL8-vKooRSSecUSt-hR2wUmg6dMw3JXvSuVR5BWml3rW-p97VQEoTvnHRK9MI5kIin7MPBu0_x50y56DFkS8NgJopz1krUS4AeFnB7AG2KOSfyep_CaNIvLUCvffXaV6999dp3Gbw_mufdSO4Zfgi6AN0BoOW_-0BJZxtosuRCWjprF8P_3H8B5VaL3g</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Thakur, A.</creator><creator>Sase, M.</creator><creator>Lee, J.J.</creator><creator>Thakur, V.</creator><creator>Buchmiller, T.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Effect of dexamethasone on insulin-like growth factor–1 expression in a rabbit model of growth retardation</title><author>Thakur, A. ; Sase, M. ; Lee, J.J. ; Thakur, V. ; Buchmiller, T.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-4dd734f83f33573a83dda5e4919d67f304c4cb6f6e9f217401f8d4d7191dd0433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Body Weight</topic><topic>dexamethasone</topic><topic>Dexamethasone - pharmacology</topic><topic>Female</topic><topic>Fetal Growth Retardation - metabolism</topic><topic>fetus</topic><topic>Fetus - metabolism</topic><topic>Glucocorticoids - pharmacology</topic><topic>Insulin-Like Growth Factor I - biosynthesis</topic><topic>insulin-like growth factor–1 (IGF-1)</topic><topic>Intrauterine growth retardation (IUGR)</topic><topic>Pregnancy</topic><topic>Rabbits</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thakur, A.</creatorcontrib><creatorcontrib>Sase, M.</creatorcontrib><creatorcontrib>Lee, J.J.</creatorcontrib><creatorcontrib>Thakur, V.</creatorcontrib><creatorcontrib>Buchmiller, T.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pediatric surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thakur, A.</au><au>Sase, M.</au><au>Lee, J.J.</au><au>Thakur, V.</au><au>Buchmiller, T.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dexamethasone on insulin-like growth factor–1 expression in a rabbit model of growth retardation</atitle><jtitle>Journal of pediatric surgery</jtitle><addtitle>J Pediatr Surg</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>35</volume><issue>6</issue><spage>898</spage><epage>905</epage><pages>898-905</pages><issn>0022-3468</issn><eissn>1531-5037</eissn><abstract>Background/Purpose: The maternal administration of steroids promotes fetal maturative effects in the gastrointestinal tract. To determine if fetal insulin-like growth factor–1 (IGF-1) expression is altered in response to maternal dexamethasone administration, this rabbit model of intrauterine growth retardation (IUGR) was utilized. Methods: Eight pregnant rabbits received either dexamethasone (Dex 0.1 mg/kg/d intramuscular), or normal saline (Cont) on gestational days 26 and 27. Fetuses were harvested on gestational day 28 or 29 and were identified as favored (Fav) or runt (Runt): DexFav, DexRunt, ContFav, and ContRunt. Fetal weight was recorded and the serum, amniotic fluid, liver, kidney, and small intestine (SI) were collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure IGF-1/β-actin mRNA densitometric band ratios in all tissues. Radioimmunoassay (RIA) was used to measure IGF-1 protein levels in the serum and amniotic fluid. Results: Weight was decreased in the Runt fetuses at all time-points (P <.08). The percent weight accretion from day 28 to 29, was greatest in the DexRunt fetus (P <.001), suggesting “catch-up” growth. All Dex fetuses (Fav and Runt) had increased liver and proximal, middle and distal SI IGF-1 mRNA at day 28 and elevated levels in the liver, proximal and distal SI at day 29 compared with control fetuses. The DexRunt fetuses had serum IGF-1 protein surpassing that of the DexFav fetus at day 28. Conclusions: This report provides the first description of maternal steroid administration effecting a marked increase in fetal IGF-1 mRNA expression and IGF-1 protein levels in an in vivo rabbit model of IUGR. The growth-retarded fetus appears to be particularly responsive. J Pediatr Surg 35:898-905. Copyright © 2000 by W.B. Saunders Company.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10873033</pmid><doi>10.1053/jpsu.2000.6914</doi><tpages>8</tpages></addata></record> |
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| subjects | Actins - metabolism Animals Body Weight dexamethasone Dexamethasone - pharmacology Female Fetal Growth Retardation - metabolism fetus Fetus - metabolism Glucocorticoids - pharmacology Insulin-Like Growth Factor I - biosynthesis insulin-like growth factor–1 (IGF-1) Intrauterine growth retardation (IUGR) Pregnancy Rabbits Reverse Transcriptase Polymerase Chain Reaction small intestine |
| title | Effect of dexamethasone on insulin-like growth factor–1 expression in a rabbit model of growth retardation |
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