Intrascleral Concentration vs Depth Profile of Mitomycin-C after Episcleral Application: Impact of Applied Concentration and Volume of Mitomycin-C Solution
The purpose of this study was to investigate the impact of different concentrations and volumes of Mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. The episcleral sides of scleral quadrants of human donor eyes were exposed for 1min to sponges (cor...
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| Veröffentlicht in: | Experimental eye research 2000-05, Vol.70 (5), p.571-575 |
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| creator | Vass, C Georgopoulos, M El Menyawi, I Radda, S Nimmerrichter, P |
| description | The purpose of this study was to investigate the impact of different concentrations and volumes of Mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. The episcleral sides of scleral quadrants of human donor eyes were exposed for 1min to sponges (corneal light shield, Merocel Corp.) soaked with MMC. After irrigation with 40ml saline a central 8mm diameter scleral disk was horizontally dissected with a cryotome. MMC concentrations of six layers of 140μ m thickness were analysed by means of high-performance liquid chromatography. In Experiment 1 (11 eyes) the sponges were soaked with 50μ l of 10, 100 and 200μ g ml−1MMC solutions. In Experiment 2 (12 eyes) the sponges were soaked with 10, 30, 50 and 80μ l of a 200μ g ml−1isotonic MMC solution.
In Experiment 1 the MMC concentrations (μ gg−1) of layer 1 were 0.35 (±0.20; 10μ gml−1group) and 9.22 (±2.92; 200μ gml−1group). In Experiment 2 the MMC concentrations were 2.57 (±1.17; 10μ l group), 7.35 (±2.49; 30μ l group) and 11.67 (±3.25; 80μ l group). The scleral MMC concentrations were significantly influenced by the applied concentrations (layers 1–5) and by the applied volumes (all layers) of MMC solution.
The intrascleral MMC concentration increased linearly with increasing concentration and not linearly with increasing volume of the applied MMC solution. To achieve more predictable scleral concentrations of MMC after trabeculectomy with MMC it seems advisable to control both the concentration and the volume of the MMC solution used to soak the sponge. |
| doi_str_mv | 10.1006/exer.1999.0816 |
| format | Article |
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In Experiment 1 the MMC concentrations (μ gg−1) of layer 1 were 0.35 (±0.20; 10μ gml−1group) and 9.22 (±2.92; 200μ gml−1group). In Experiment 2 the MMC concentrations were 2.57 (±1.17; 10μ l group), 7.35 (±2.49; 30μ l group) and 11.67 (±3.25; 80μ l group). The scleral MMC concentrations were significantly influenced by the applied concentrations (layers 1–5) and by the applied volumes (all layers) of MMC solution.
The intrascleral MMC concentration increased linearly with increasing concentration and not linearly with increasing volume of the applied MMC solution. To achieve more predictable scleral concentrations of MMC after trabeculectomy with MMC it seems advisable to control both the concentration and the volume of the MMC solution used to soak the sponge.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1006/exer.1999.0816</identifier><identifier>PMID: 10870515</identifier><identifier>CODEN: EXERA6</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Absorption ; Administration, Topical ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; ciliary body ; complication ; concentration ; Dose-Response Relationship, Drug ; Eye ; filtering surgery ; Glaucoma ; Glaucoma - metabolism ; Glaucoma - surgery ; Humans ; Medical sciences ; Mitomycin - pharmacokinetics ; mitomycin-C ; Nucleic Acid Synthesis Inhibitors - pharmacokinetics ; Pharmacology. Drug treatments ; Sclera - drug effects ; Sclera - metabolism ; toxicity ; Trabeculectomy</subject><ispartof>Experimental eye research, 2000-05, Vol.70 (5), p.571-575</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-c4bf33882c516c66a69ece94c8531d36d68938b7615ff89d88cba33ee576e0993</citedby><cites>FETCH-LOGICAL-c369t-c4bf33882c516c66a69ece94c8531d36d68938b7615ff89d88cba33ee576e0993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/exer.1999.0816$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1414928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10870515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vass, C</creatorcontrib><creatorcontrib>Georgopoulos, M</creatorcontrib><creatorcontrib>El Menyawi, I</creatorcontrib><creatorcontrib>Radda, S</creatorcontrib><creatorcontrib>Nimmerrichter, P</creatorcontrib><title>Intrascleral Concentration vs Depth Profile of Mitomycin-C after Episcleral Application: Impact of Applied Concentration and Volume of Mitomycin-C Solution</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The purpose of this study was to investigate the impact of different concentrations and volumes of Mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. The episcleral sides of scleral quadrants of human donor eyes were exposed for 1min to sponges (corneal light shield, Merocel Corp.) soaked with MMC. After irrigation with 40ml saline a central 8mm diameter scleral disk was horizontally dissected with a cryotome. MMC concentrations of six layers of 140μ m thickness were analysed by means of high-performance liquid chromatography. In Experiment 1 (11 eyes) the sponges were soaked with 50μ l of 10, 100 and 200μ g ml−1MMC solutions. In Experiment 2 (12 eyes) the sponges were soaked with 10, 30, 50 and 80μ l of a 200μ g ml−1isotonic MMC solution.
In Experiment 1 the MMC concentrations (μ gg−1) of layer 1 were 0.35 (±0.20; 10μ gml−1group) and 9.22 (±2.92; 200μ gml−1group). In Experiment 2 the MMC concentrations were 2.57 (±1.17; 10μ l group), 7.35 (±2.49; 30μ l group) and 11.67 (±3.25; 80μ l group). The scleral MMC concentrations were significantly influenced by the applied concentrations (layers 1–5) and by the applied volumes (all layers) of MMC solution.
The intrascleral MMC concentration increased linearly with increasing concentration and not linearly with increasing volume of the applied MMC solution. To achieve more predictable scleral concentrations of MMC after trabeculectomy with MMC it seems advisable to control both the concentration and the volume of the MMC solution used to soak the sponge.</description><subject>Absorption</subject><subject>Administration, Topical</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>ciliary body</subject><subject>complication</subject><subject>concentration</subject><subject>Dose-Response Relationship, Drug</subject><subject>Eye</subject><subject>filtering surgery</subject><subject>Glaucoma</subject><subject>Glaucoma - metabolism</subject><subject>Glaucoma - surgery</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mitomycin - pharmacokinetics</subject><subject>mitomycin-C</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Sclera - drug effects</subject><subject>Sclera - metabolism</subject><subject>toxicity</subject><subject>Trabeculectomy</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9vFCEcxUmjadfqtUfDwXibFRaGgd6ateomNW3ijythmS8phhlGYBv7t_jPynTXaGo8kbx83gPeQ-iMkiUlRLyBH5CWVCm1JJKKI7SgRImGENI9QQtCKG-4ZO0Jepbzt6oy3vFjdEKJ7EhL2wX6uRlLMtkGSCbgdRwtzELxccR3Gb-FqdzimxSdD4Cjwx99icO99WOzxsYVSPhy8r_tF9MUvH0wn-PNMBlbZs-DDP2jdDP2-GsMu-Gf3E9VnZHn6KkzIcOLw3mKvry7_Lz-0Fxdv9-sL64ay4QqjeVbx5iUK9tSYYUwQoEFxa1sGe2Z6IVUTG47QVvnpOqltFvDGEDbCSBKsVP0ep87pfh9B7nooX4JQjAjxF3WHV2Rjq9IBZd70KaYcwKnp-QHk-41JXqeQ89z6HkOPc9RDS8PybvtAP1f-L7_Crw6AHUDE1wyo_X5D8cpVytZMbnHoNZw5-sd2XqobfY-gS26j_5_T_gFOfmotA</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Vass, C</creator><creator>Georgopoulos, M</creator><creator>El Menyawi, I</creator><creator>Radda, S</creator><creator>Nimmerrichter, P</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Intrascleral Concentration vs Depth Profile of Mitomycin-C after Episcleral Application: Impact of Applied Concentration and Volume of Mitomycin-C Solution</title><author>Vass, C ; Georgopoulos, M ; El Menyawi, I ; Radda, S ; Nimmerrichter, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-c4bf33882c516c66a69ece94c8531d36d68938b7615ff89d88cba33ee576e0993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Absorption</topic><topic>Administration, Topical</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>ciliary body</topic><topic>complication</topic><topic>concentration</topic><topic>Dose-Response Relationship, Drug</topic><topic>Eye</topic><topic>filtering surgery</topic><topic>Glaucoma</topic><topic>Glaucoma - metabolism</topic><topic>Glaucoma - surgery</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mitomycin - pharmacokinetics</topic><topic>mitomycin-C</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Sclera - drug effects</topic><topic>Sclera - metabolism</topic><topic>toxicity</topic><topic>Trabeculectomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vass, C</creatorcontrib><creatorcontrib>Georgopoulos, M</creatorcontrib><creatorcontrib>El Menyawi, I</creatorcontrib><creatorcontrib>Radda, S</creatorcontrib><creatorcontrib>Nimmerrichter, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vass, C</au><au>Georgopoulos, M</au><au>El Menyawi, I</au><au>Radda, S</au><au>Nimmerrichter, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrascleral Concentration vs Depth Profile of Mitomycin-C after Episcleral Application: Impact of Applied Concentration and Volume of Mitomycin-C Solution</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>70</volume><issue>5</issue><spage>571</spage><epage>575</epage><pages>571-575</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><coden>EXERA6</coden><abstract>The purpose of this study was to investigate the impact of different concentrations and volumes of Mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. The episcleral sides of scleral quadrants of human donor eyes were exposed for 1min to sponges (corneal light shield, Merocel Corp.) soaked with MMC. After irrigation with 40ml saline a central 8mm diameter scleral disk was horizontally dissected with a cryotome. MMC concentrations of six layers of 140μ m thickness were analysed by means of high-performance liquid chromatography. In Experiment 1 (11 eyes) the sponges were soaked with 50μ l of 10, 100 and 200μ g ml−1MMC solutions. In Experiment 2 (12 eyes) the sponges were soaked with 10, 30, 50 and 80μ l of a 200μ g ml−1isotonic MMC solution.
In Experiment 1 the MMC concentrations (μ gg−1) of layer 1 were 0.35 (±0.20; 10μ gml−1group) and 9.22 (±2.92; 200μ gml−1group). In Experiment 2 the MMC concentrations were 2.57 (±1.17; 10μ l group), 7.35 (±2.49; 30μ l group) and 11.67 (±3.25; 80μ l group). The scleral MMC concentrations were significantly influenced by the applied concentrations (layers 1–5) and by the applied volumes (all layers) of MMC solution.
The intrascleral MMC concentration increased linearly with increasing concentration and not linearly with increasing volume of the applied MMC solution. To achieve more predictable scleral concentrations of MMC after trabeculectomy with MMC it seems advisable to control both the concentration and the volume of the MMC solution used to soak the sponge.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>10870515</pmid><doi>10.1006/exer.1999.0816</doi><tpages>5</tpages></addata></record> |
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| ispartof | Experimental eye research, 2000-05, Vol.70 (5), p.571-575 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Absorption Administration, Topical Biological and medical sciences Chromatography, High Pressure Liquid ciliary body complication concentration Dose-Response Relationship, Drug Eye filtering surgery Glaucoma Glaucoma - metabolism Glaucoma - surgery Humans Medical sciences Mitomycin - pharmacokinetics mitomycin-C Nucleic Acid Synthesis Inhibitors - pharmacokinetics Pharmacology. Drug treatments Sclera - drug effects Sclera - metabolism toxicity Trabeculectomy |
| title | Intrascleral Concentration vs Depth Profile of Mitomycin-C after Episcleral Application: Impact of Applied Concentration and Volume of Mitomycin-C Solution |
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