USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE
Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection in swine. The antigens were evaluated with sera from young pigs that had been fed 1–10,000 T. gondii...
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| Veröffentlicht in: | The Journal of parasitology 2000-06, Vol.86 (3), p.459-462 |
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| creator | Gamble, H. Ray Andrews, Carolyn D Dubey, J. P Webert, Donald W Parmley, Stephen F |
| description | Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection in swine. The antigens were evaluated with sera from young pigs that had been fed 1–10,000 T. gondii oocysts of the VEG or GT-1 strains. Results were compared with an EIA using a native T. gondii antigen extract. All 5 recombinant antigens, as well as native antigen, detected antibody responses as soon as 3 wk after infection in pigs inoculated with 1 or 10 oocysts of the VEG strain. This antibody response persisted, at varying levels, for 14 wk when the experiment was terminated. All antigens also detected antibody responses in pigs 4 wk after inoculation with 10,000 oocysts of the GT-1 strain. The antibody response recognized by native antigen remained high through 51 wk after inoculation. However, there was considerable animal-to-animal variation in responses to the individual recombinant antigens. Only antigens C51 and MBP30 consistently detected a positive antibody response over the entire 51-wk course of the experiment. These results suggest that these antigens might be useful for the serological detection of T. gondii infection in pigs. |
| doi_str_mv | 10.1645/0022-3395(2000)086[0459:UORAFD]2.0.CO;2 |
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Ray ; Andrews, Carolyn D ; Dubey, J. P ; Webert, Donald W ; Parmley, Stephen F</creator><creatorcontrib>Gamble, H. Ray ; Andrews, Carolyn D ; Dubey, J. P ; Webert, Donald W ; Parmley, Stephen F</creatorcontrib><description>Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection in swine. The antigens were evaluated with sera from young pigs that had been fed 1–10,000 T. gondii oocysts of the VEG or GT-1 strains. Results were compared with an EIA using a native T. gondii antigen extract. All 5 recombinant antigens, as well as native antigen, detected antibody responses as soon as 3 wk after infection in pigs inoculated with 1 or 10 oocysts of the VEG strain. This antibody response persisted, at varying levels, for 14 wk when the experiment was terminated. All antigens also detected antibody responses in pigs 4 wk after inoculation with 10,000 oocysts of the GT-1 strain. The antibody response recognized by native antigen remained high through 51 wk after inoculation. However, there was considerable animal-to-animal variation in responses to the individual recombinant antigens. Only antigens C51 and MBP30 consistently detected a positive antibody response over the entire 51-wk course of the experiment. 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Ray</creatorcontrib><creatorcontrib>Andrews, Carolyn D</creatorcontrib><creatorcontrib>Dubey, J. P</creatorcontrib><creatorcontrib>Webert, Donald W</creatorcontrib><creatorcontrib>Parmley, Stephen F</creatorcontrib><title>USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection in swine. The antigens were evaluated with sera from young pigs that had been fed 1–10,000 T. gondii oocysts of the VEG or GT-1 strains. Results were compared with an EIA using a native T. gondii antigen extract. All 5 recombinant antigens, as well as native antigen, detected antibody responses as soon as 3 wk after infection in pigs inoculated with 1 or 10 oocysts of the VEG strain. This antibody response persisted, at varying levels, for 14 wk when the experiment was terminated. All antigens also detected antibody responses in pigs 4 wk after inoculation with 10,000 oocysts of the GT-1 strain. The antibody response recognized by native antigen remained high through 51 wk after inoculation. However, there was considerable animal-to-animal variation in responses to the individual recombinant antigens. Only antigens C51 and MBP30 consistently detected a positive antibody response over the entire 51-wk course of the experiment. These results suggest that these antigens might be useful for the serological detection of T. gondii infection in pigs.</description><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Cysts</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Environmental impact assessment</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>IMMUNOLOGY</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Oocysts</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Protozoal diseases</subject><subject>Recombinant antigens</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - immunology</subject><subject>Tachyzoites</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis, Animal - diagnosis</subject><subject>Toxoplasmosis, Animal - immunology</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqdkE1r20AQhpeSkrhJ_kLRoYT0IGe_tJKakyJL7oKjTWyZFkpZdtcrsLGtVGsf-u-7QqbJOYdhDvPMO8MDwB2CY8RodAchxiEhaXSLIYRfYcJ-QRql35ZinpWT33gMx7m4xx_ACKUkDjGh0RkY_d-6AJ-c2_jNyNc5uEA-gGIKR-B5uSgCUQbzIhePD7zKqjrwxadFtQhKMQ8mRV3kNRdVT9Xip3iaZYvHLJiKasJ5wKvyNOZVsPjBq-IKfGzU1tnrU78Ey7Ko8-_hTEx5ns1CTWJ2CJHGNFLWKM0MtlZjaJOGsaRJKTNsReNG6WgFjYHIGKV0qhjS1MY4TQlhNiWX4GbIfenaP0frDnK3dsZut2pv26OTMcJeUYw8OB1A07XOdbaRL916p7q_EkHZ25W9J9l7kr1d6eXI3q4c7EosocyFxD7p8-nkUe_s6k3OoNMDX06AckZtm07tzdq9ch7CkL1iG3dou7fvYAJjSXBCkyj2WDFget22e_vut_8BfVyeFA</recordid><startdate>200006</startdate><enddate>200006</enddate><creator>Gamble, H. Ray</creator><creator>Andrews, Carolyn D</creator><creator>Dubey, J. P</creator><creator>Webert, Donald W</creator><creator>Parmley, Stephen F</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200006</creationdate><title>USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE</title><author>Gamble, H. Ray ; Andrews, Carolyn D ; Dubey, J. P ; Webert, Donald W ; Parmley, Stephen F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b376t-1b245aecab6c2eeb20e8f668f946c6d47fab5d0cc01ccaab9a61b4e7299336e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animal protozoal diseases</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antigens</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Cysts</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Environmental impact assessment</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>IMMUNOLOGY</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Oocysts</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>Protozoal diseases</topic><topic>Recombinant antigens</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - immunology</topic><topic>Tachyzoites</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasmosis</topic><topic>Toxoplasmosis, Animal - diagnosis</topic><topic>Toxoplasmosis, Animal - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gamble, H. Ray</creatorcontrib><creatorcontrib>Andrews, Carolyn D</creatorcontrib><creatorcontrib>Dubey, J. P</creatorcontrib><creatorcontrib>Webert, Donald W</creatorcontrib><creatorcontrib>Parmley, Stephen F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gamble, H. Ray</au><au>Andrews, Carolyn D</au><au>Dubey, J. P</au><au>Webert, Donald W</au><au>Parmley, Stephen F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2000-06</date><risdate>2000</risdate><volume>86</volume><issue>3</issue><spage>459</spage><epage>462</epage><pages>459-462</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Five recombinant Toxoplasma gondii antigens, designated B427, C51, C55, V22, and MBP30 were assessed for their potential use in an enzyme-linked immunoassay (EIA) for detection of T. gondii infection in swine. The antigens were evaluated with sera from young pigs that had been fed 1–10,000 T. gondii oocysts of the VEG or GT-1 strains. Results were compared with an EIA using a native T. gondii antigen extract. All 5 recombinant antigens, as well as native antigen, detected antibody responses as soon as 3 wk after infection in pigs inoculated with 1 or 10 oocysts of the VEG strain. This antibody response persisted, at varying levels, for 14 wk when the experiment was terminated. All antigens also detected antibody responses in pigs 4 wk after inoculation with 10,000 oocysts of the GT-1 strain. The antibody response recognized by native antigen remained high through 51 wk after inoculation. However, there was considerable animal-to-animal variation in responses to the individual recombinant antigens. Only antigens C51 and MBP30 consistently detected a positive antibody response over the entire 51-wk course of the experiment. These results suggest that these antigens might be useful for the serological detection of T. gondii infection in pigs.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>10864240</pmid><doi>10.1645/0022-3395(2000)086[0459:UORAFD]2.0.CO;2</doi><tpages>4</tpages></addata></record> |
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| ispartof | The Journal of parasitology, 2000-06, Vol.86 (3), p.459-462 |
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| subjects | Animal protozoal diseases Animals Antibodies Antibodies, Protozoan - biosynthesis Antibodies, Protozoan - blood Antigens Antigens, Protozoan - genetics Antigens, Protozoan - immunology Biological and medical sciences Biological Assay Cysts Dose-Response Relationship, Immunologic Environmental impact assessment Enzyme-Linked Immunosorbent Assay - veterinary IMMUNOLOGY Infections Infectious diseases Medical sciences Oocysts Parasitic diseases Parasitology Protozoal diseases Recombinant antigens Recombinant Proteins - genetics Recombinant Proteins - immunology Swine Swine Diseases - diagnosis Swine Diseases - immunology Tachyzoites Toxoplasma - immunology Toxoplasmosis Toxoplasmosis, Animal - diagnosis Toxoplasmosis, Animal - immunology |
| title | USE OF RECOMBINANT ANTIGENS FOR DETECTION OF TOXOPLASMA GONDII INFECTION IN SWINE |
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