Role of Acidic Amino Acid Residues of PsaD Subunit on Limiting the Affinity of Photosystem I for Ferredoxin
The PsaD subunit of photosystem I is one of the central polypeptides for the interaction with ferredoxin, its acidic electron acceptor. In the cyanobacterium Synechocystis 6803, this role is partly performed by a sequence extending approximately from histidine 97 to arginine 119, close to the C-term...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2001-10, Vol.287 (4), p.833-836 |
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| creator | Bottin, Hervé Hanley, Jonathan Lagoutte, Bernard |
| description | The PsaD subunit of photosystem I is one of the central polypeptides for the interaction with ferredoxin, its acidic electron acceptor. In the cyanobacterium Synechocystis 6803, this role is partly performed by a sequence extending approximately from histidine 97 to arginine 119, close to the C-terminus. In the present work, acidic amino acids D100, E105, and E109 are shown to moderate the affinity of Photosystem I for ferredoxin. Most single replacements of these residues by neutral amino acids increased the affinity for ferredoxin, resulting in a dissociation constant as low as 0.015 μM for the E105Q mutant (wild-type KD = 0.4 μM). This is the first report on the limitation of photosystem I affinity for ferredoxin due to acidic amino acids from PsaD subunit. It highlights the occurrence of a negative control on the binding during the formation of transient complexes between electron carriers. |
| doi_str_mv | 10.1006/bbrc.2001.5658 |
| format | Article |
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In the cyanobacterium Synechocystis 6803, this role is partly performed by a sequence extending approximately from histidine 97 to arginine 119, close to the C-terminus. In the present work, acidic amino acids D100, E105, and E109 are shown to moderate the affinity of Photosystem I for ferredoxin. Most single replacements of these residues by neutral amino acids increased the affinity for ferredoxin, resulting in a dissociation constant as low as 0.015 μM for the E105Q mutant (wild-type KD = 0.4 μM). This is the first report on the limitation of photosystem I affinity for ferredoxin due to acidic amino acids from PsaD subunit. 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In the cyanobacterium Synechocystis 6803, this role is partly performed by a sequence extending approximately from histidine 97 to arginine 119, close to the C-terminus. In the present work, acidic amino acids D100, E105, and E109 are shown to moderate the affinity of Photosystem I for ferredoxin. Most single replacements of these residues by neutral amino acids increased the affinity for ferredoxin, resulting in a dissociation constant as low as 0.015 μM for the E105Q mutant (wild-type KD = 0.4 μM). This is the first report on the limitation of photosystem I affinity for ferredoxin due to acidic amino acids from PsaD subunit. It highlights the occurrence of a negative control on the binding during the formation of transient complexes between electron carriers.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cyanobacteria - chemistry</subject><subject>Cyanobacteria - genetics</subject><subject>cyanobacterium</subject><subject>Cyanophyta</subject><subject>electron transfer</subject><subject>ferredoxin</subject><subject>Ferredoxins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Oxidation-Reduction</subject><subject>Photosynthetic Reaction Center Complex Proteins - chemistry</subject><subject>photosystem I</subject><subject>Photosystem I Protein Complex</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Binding</subject><subject>PsaD protein</subject><subject>PsaD subunit</subject><subject>Synechocystis</subject><subject>Synechocystis sp. 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PCC 6803</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bottin, Hervé</creatorcontrib><creatorcontrib>Hanley, Jonathan</creatorcontrib><creatorcontrib>Lagoutte, Bernard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bottin, Hervé</au><au>Hanley, Jonathan</au><au>Lagoutte, Bernard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Acidic Amino Acid Residues of PsaD Subunit on Limiting the Affinity of Photosystem I for Ferredoxin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2001-10-05</date><risdate>2001</risdate><volume>287</volume><issue>4</issue><spage>833</spage><epage>836</epage><pages>833-836</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The PsaD subunit of photosystem I is one of the central polypeptides for the interaction with ferredoxin, its acidic electron acceptor. 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| ispartof | Biochemical and biophysical research communications, 2001-10, Vol.287 (4), p.833-836 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Cyanobacteria - chemistry Cyanobacteria - genetics cyanobacterium Cyanophyta electron transfer ferredoxin Ferredoxins - metabolism Molecular Sequence Data Oxidation-Reduction Photosynthetic Reaction Center Complex Proteins - chemistry photosystem I Photosystem I Protein Complex Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Protein Binding PsaD protein PsaD subunit Synechocystis Synechocystis sp. PCC 6803 |
| title | Role of Acidic Amino Acid Residues of PsaD Subunit on Limiting the Affinity of Photosystem I for Ferredoxin |
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