Rapid duplex PCR assay for the detection of pathogenic Yersinia enterocolitica strains

For the detection of pathogenic Yersinia enterocolitica strains, a duplex PCR has been developed based on differences observed between the fingerprint profiles of pathogenic and non-pathogenic strains. The profiles were obtained by using a primer derived from the Enterobacterial Repetitive Intergeni...

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Veröffentlicht in:	Journal of microbiological methods 2001-11, Vol.47 (2), p.209-217
Hauptverfasser:	Aarts, Henk J.M, Joosten, Ruth G, Henkens, Marleen H.C, Stegeman, Henk, van Hoek, Angela H.A.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences bipA gene DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - genetics Duplex PCR ERIC-PCR fingerprinting Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data Nucleic Acid Denaturation Pathogenic strains Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase Chain Reaction - methods Real-time PCR Yersinia enterocolitica Sensitivity and Specificity Taq Polymerase - metabolism Yersinia enterocolitica Yersinia enterocolitica - chemistry Yersinia enterocolitica - genetics Yersinia enterocolitica - isolation & purification Yersinia enterocolitica - pathogenicity Yersinia Infections - microbiology
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