Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity in the ureter of the duck
The presence, distribution and colocalisation of pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity have been studied in the duck ureter by using Western blot analysis, radioimmunoassays (RIA) and immunohistochemistry. The presence of both PACAP-38 and PACAP-27 was demonstrated,...
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| Veröffentlicht in: | Cell and tissue research 2001-09, Vol.305 (3), p.341-349 |
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| creator | Mirabella, N Squillacioti, C Germano, G Varricchio, E Paino, G |
| description | The presence, distribution and colocalisation of pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity have been studied in the duck ureter by using Western blot analysis, radioimmunoassays (RIA) and immunohistochemistry. The presence of both PACAP-38 and PACAP-27 was demonstrated, PACAP-38 being the predominant form. PACAP-immunoreactive fibres and neurons were found in all the ureteral layers. Double immunostaining showed that PACAP was almost completely colocalised with vasoactive intestinal peptide (VIP). Moreover, PACAP was found in substance P (SP)-containing ureteral nerve fibres and in SP-containing dorsal root ganglion neurons. RIA performed on denervated ureters demonstrated that almost half of the ureteral PACAP was extrinsic in origin. These findings suggest that, in birds, PACAP has a role in diverse nerve-mediated ureteral functions. |
| doi_str_mv | 10.1007/s004410100415 |
| format | Article |
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The presence of both PACAP-38 and PACAP-27 was demonstrated, PACAP-38 being the predominant form. PACAP-immunoreactive fibres and neurons were found in all the ureteral layers. Double immunostaining showed that PACAP was almost completely colocalised with vasoactive intestinal peptide (VIP). Moreover, PACAP was found in substance P (SP)-containing ureteral nerve fibres and in SP-containing dorsal root ganglion neurons. RIA performed on denervated ureters demonstrated that almost half of the ureteral PACAP was extrinsic in origin. These findings suggest that, in birds, PACAP has a role in diverse nerve-mediated ureteral functions.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s004410100415</identifier><identifier>PMID: 11572087</identifier><language>eng</language><publisher>Germany</publisher><subject>Anas ; Anatidae ; Animals ; Antibodies ; Blotting, Western ; Calcitonin Gene-Related Peptide - analysis ; Calcitonin Gene-Related Peptide - immunology ; Ducks ; Female ; Immunohistochemistry ; Male ; Neuropeptides - analysis ; Neuropeptides - immunology ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Radioimmunoassay ; Substance P - analysis ; Substance P - immunology ; Ureter - chemistry ; Vasoactive Intestinal Peptide - analysis</subject><ispartof>Cell and tissue research, 2001-09, Vol.305 (3), p.341-349</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-89d7d3330608a45ed8d210d152290067439d8b79691dc8af92c04cadfa80610b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11572087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mirabella, N</creatorcontrib><creatorcontrib>Squillacioti, C</creatorcontrib><creatorcontrib>Germano, G</creatorcontrib><creatorcontrib>Varricchio, E</creatorcontrib><creatorcontrib>Paino, G</creatorcontrib><title>Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity in the ureter of the duck</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>The presence, distribution and colocalisation of pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity have been studied in the duck ureter by using Western blot analysis, radioimmunoassays (RIA) and immunohistochemistry. The presence of both PACAP-38 and PACAP-27 was demonstrated, PACAP-38 being the predominant form. PACAP-immunoreactive fibres and neurons were found in all the ureteral layers. Double immunostaining showed that PACAP was almost completely colocalised with vasoactive intestinal peptide (VIP). Moreover, PACAP was found in substance P (SP)-containing ureteral nerve fibres and in SP-containing dorsal root ganglion neurons. RIA performed on denervated ureters demonstrated that almost half of the ureteral PACAP was extrinsic in origin. These findings suggest that, in birds, PACAP has a role in diverse nerve-mediated ureteral functions.</description><subject>Anas</subject><subject>Anatidae</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Blotting, Western</subject><subject>Calcitonin Gene-Related Peptide - analysis</subject><subject>Calcitonin Gene-Related Peptide - immunology</subject><subject>Ducks</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Neuropeptides - analysis</subject><subject>Neuropeptides - immunology</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Radioimmunoassay</subject><subject>Substance P - analysis</subject><subject>Substance P - immunology</subject><subject>Ureter - chemistry</subject><subject>Vasoactive Intestinal Peptide - analysis</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1LAzEQgOEgiq3Vo1fJSfSwOpNkd5NjKX5BwR4UvC1pktXoftRNVui_d20L4snTzMDDHF5CThGuECC_DgBCIAy7wHSPjFFwloDM5T4ZAweW5Fn2MiJHIbwDoMgydUhGiGnOBjQmeuFj76Pu1lRb16wrHR01a1Pp4Kg20X_p6JtXunKr6K2jF4vpbLq4pL6u-6bt3Ib4uKa-ofHN0b5z0XW0LTeX7c3HMTkodRXcyW5OyPPtzdPsPpk_3j3MpvPEcAYxkcrmlnMOGUgtUmelZQgWU8YUQJYLrqxc5ipTaI3UpWIGhNG21BIyhCWfkPPt31XXfvYuxKL2wbiq0o1r-1DkiEopgf9ClDxNU2QDTLbQdG0InSuLVefrIVWBUPzEL_7EH_zZ7nG_rJ391bva_BsobX5I</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Mirabella, N</creator><creator>Squillacioti, C</creator><creator>Germano, G</creator><creator>Varricchio, E</creator><creator>Paino, G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity in the ureter of the duck</title><author>Mirabella, N ; Squillacioti, C ; Germano, G ; Varricchio, E ; Paino, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-89d7d3330608a45ed8d210d152290067439d8b79691dc8af92c04cadfa80610b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Anas</topic><topic>Anatidae</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Blotting, Western</topic><topic>Calcitonin Gene-Related Peptide - analysis</topic><topic>Calcitonin Gene-Related Peptide - immunology</topic><topic>Ducks</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Neuropeptides - analysis</topic><topic>Neuropeptides - immunology</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Radioimmunoassay</topic><topic>Substance P - analysis</topic><topic>Substance P - immunology</topic><topic>Ureter - chemistry</topic><topic>Vasoactive Intestinal Peptide - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mirabella, N</creatorcontrib><creatorcontrib>Squillacioti, C</creatorcontrib><creatorcontrib>Germano, G</creatorcontrib><creatorcontrib>Varricchio, E</creatorcontrib><creatorcontrib>Paino, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mirabella, N</au><au>Squillacioti, C</au><au>Germano, G</au><au>Varricchio, E</au><au>Paino, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity in the ureter of the duck</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>305</volume><issue>3</issue><spage>341</spage><epage>349</epage><pages>341-349</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>The presence, distribution and colocalisation of pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity have been studied in the duck ureter by using Western blot analysis, radioimmunoassays (RIA) and immunohistochemistry. The presence of both PACAP-38 and PACAP-27 was demonstrated, PACAP-38 being the predominant form. PACAP-immunoreactive fibres and neurons were found in all the ureteral layers. Double immunostaining showed that PACAP was almost completely colocalised with vasoactive intestinal peptide (VIP). Moreover, PACAP was found in substance P (SP)-containing ureteral nerve fibres and in SP-containing dorsal root ganglion neurons. RIA performed on denervated ureters demonstrated that almost half of the ureteral PACAP was extrinsic in origin. These findings suggest that, in birds, PACAP has a role in diverse nerve-mediated ureteral functions.</abstract><cop>Germany</cop><pmid>11572087</pmid><doi>10.1007/s004410100415</doi><tpages>9</tpages></addata></record> |
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| ispartof | Cell and tissue research, 2001-09, Vol.305 (3), p.341-349 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Anas Anatidae Animals Antibodies Blotting, Western Calcitonin Gene-Related Peptide - analysis Calcitonin Gene-Related Peptide - immunology Ducks Female Immunohistochemistry Male Neuropeptides - analysis Neuropeptides - immunology Pituitary Adenylate Cyclase-Activating Polypeptide Radioimmunoassay Substance P - analysis Substance P - immunology Ureter - chemistry Vasoactive Intestinal Peptide - analysis |
| title | Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity in the ureter of the duck |
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