Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro
Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro using a culture system that supports the developm...
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| Veröffentlicht in: | Biology of reproduction 2000-07, Vol.63 (1), p.320-327 |
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| creator | JI WU NAYUDU, P. L KIESEL, P. S MICHELMANN, H. W |
| description | Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle
development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro
using a culture system that supports the development of intact follicles. We have previously shown that although preantral
follicles 150 μm in diameter (2â3 granulosa cell layers) do not require LH to proceed through antral development, smaller
follicles (1â2 granulosa cell layers, 85â110 μm in diameter) do not develop beyond the large preantral stage in the presence
of only FSH and 5% mouse serum. Follicles of this size were therefore used to determine the effects of LH and serum on their
development in vitro. The results showed that although FSH must be continuously present, a low concentration of LH together
with a slight increase in serum concentration was necessary, specifically during the primary stage of follicle development
(from 85 μm in diameter until the follicles had reached 150 μm in diameter) to induce the capacity for subsequent LH-independent
rapid growth and antral development. The in vitro development of maturable oocytes with normal spindle and chromatin morphology
was also supported. These results indicate that LH probably induces changes in the early differentiating thecal cells, which
are critical for the completion of subsequent follicular and oocyte development. |
| doi_str_mv | 10.1095/biolreprod63.1.320 |
| format | Article |
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development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro
using a culture system that supports the development of intact follicles. We have previously shown that although preantral
follicles 150 μm in diameter (2â3 granulosa cell layers) do not require LH to proceed through antral development, smaller
follicles (1â2 granulosa cell layers, 85â110 μm in diameter) do not develop beyond the large preantral stage in the presence
of only FSH and 5% mouse serum. Follicles of this size were therefore used to determine the effects of LH and serum on their
development in vitro. The results showed that although FSH must be continuously present, a low concentration of LH together
with a slight increase in serum concentration was necessary, specifically during the primary stage of follicle development
(from 85 μm in diameter until the follicles had reached 150 μm in diameter) to induce the capacity for subsequent LH-independent
rapid growth and antral development. The in vitro development of maturable oocytes with normal spindle and chromatin morphology
was also supported. These results indicate that LH probably induces changes in the early differentiating thecal cells, which
are critical for the completion of subsequent follicular and oocyte development.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod63.1.320</identifier><identifier>PMID: 10859274</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cell Division - drug effects ; Cells, Cultured ; Culture Media - chemistry ; Female ; Follicle Stimulating Hormone - metabolism ; Follicle Stimulating Hormone - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Luteinizing Hormone - metabolism ; Luteinizing Hormone - pharmacology ; Mammalian female genital system ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Ovarian Follicle - drug effects ; Ovarian Follicle - physiology ; Time Factors ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2000-07, Vol.63 (1), p.320-327</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1489811$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10859274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JI WU</creatorcontrib><creatorcontrib>NAYUDU, P. L</creatorcontrib><creatorcontrib>KIESEL, P. S</creatorcontrib><creatorcontrib>MICHELMANN, H. W</creatorcontrib><title>Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle
development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro
using a culture system that supports the development of intact follicles. We have previously shown that although preantral
follicles 150 μm in diameter (2â3 granulosa cell layers) do not require LH to proceed through antral development, smaller
follicles (1â2 granulosa cell layers, 85â110 μm in diameter) do not develop beyond the large preantral stage in the presence
of only FSH and 5% mouse serum. Follicles of this size were therefore used to determine the effects of LH and serum on their
development in vitro. The results showed that although FSH must be continuously present, a low concentration of LH together
with a slight increase in serum concentration was necessary, specifically during the primary stage of follicle development
(from 85 μm in diameter until the follicles had reached 150 μm in diameter) to induce the capacity for subsequent LH-independent
rapid growth and antral development. The in vitro development of maturable oocytes with normal spindle and chromatin morphology
was also supported. These results indicate that LH probably induces changes in the early differentiating thecal cells, which
are critical for the completion of subsequent follicular and oocyte development.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Culture Media - chemistry</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - physiology</subject><subject>Time Factors</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0U9LIzEYBvAgLlq7fgEPkoN6m5o_M8nkKFqtUNgFdwVPQybzThvJJDVJLeund8DKnl54-PEcnhehM0pmlKjqurXBRdjE0Ak-ozPOyAGa0IqpQjJRH6IJIUQUnAt-jE5SeiWElpzxI3RMSV0pJssJelluM1hvP6xf4UWIQ_CAFzphjZ-yXkGxtIPN0OF534PJOHj8O4L2OWqH74Nz1jjAd_AOLmwG8Bk_evxscww_0Y9euwSn-ztFf-_nf24XxfLXw-PtzbJYMyFyAaUkUHNCW0pVa2RLoerLrmqFHOO6bDuhK6IFV0aKSjNVaS6oYJwpYriRfIquvnrHId62kHIz2GTAOe0hbFMjx16pBBnh-R5u2wG6ZhPtoOO_5nuMEVzsgU5Guz5qb2z678pa1ZSO7PKLre1qvbMRmjRo58ZW3ux2O8Eb2oy_4J-wWHv9</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>JI WU</creator><creator>NAYUDU, P. L</creator><creator>KIESEL, P. S</creator><creator>MICHELMANN, H. W</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro</title><author>JI WU ; NAYUDU, P. L ; KIESEL, P. S ; MICHELMANN, H. W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-e470e8301b119bc7b1e5f4d5b67e8384bd6a50a639c765a295a361623290c3c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Culture Media - chemistry</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - physiology</topic><topic>Time Factors</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JI WU</creatorcontrib><creatorcontrib>NAYUDU, P. L</creatorcontrib><creatorcontrib>KIESEL, P. S</creatorcontrib><creatorcontrib>MICHELMANN, H. W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JI WU</au><au>NAYUDU, P. L</au><au>KIESEL, P. S</au><au>MICHELMANN, H. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>63</volume><issue>1</issue><spage>320</spage><epage>327</epage><pages>320-327</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle
development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro
using a culture system that supports the development of intact follicles. We have previously shown that although preantral
follicles 150 μm in diameter (2â3 granulosa cell layers) do not require LH to proceed through antral development, smaller
follicles (1â2 granulosa cell layers, 85â110 μm in diameter) do not develop beyond the large preantral stage in the presence
of only FSH and 5% mouse serum. Follicles of this size were therefore used to determine the effects of LH and serum on their
development in vitro. The results showed that although FSH must be continuously present, a low concentration of LH together
with a slight increase in serum concentration was necessary, specifically during the primary stage of follicle development
(from 85 μm in diameter until the follicles had reached 150 μm in diameter) to induce the capacity for subsequent LH-independent
rapid growth and antral development. The in vitro development of maturable oocytes with normal spindle and chromatin morphology
was also supported. These results indicate that LH probably induces changes in the early differentiating thecal cells, which
are critical for the completion of subsequent follicular and oocyte development.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10859274</pmid><doi>10.1095/biolreprod63.1.320</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2000-07, Vol.63 (1), p.320-327 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Biological and medical sciences Cell Division - drug effects Cells, Cultured Culture Media - chemistry Female Follicle Stimulating Hormone - metabolism Follicle Stimulating Hormone - pharmacology Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Luteinizing Hormone - metabolism Luteinizing Hormone - pharmacology Mammalian female genital system Mice Mice, Inbred C57BL Mice, Inbred CBA Ovarian Follicle - drug effects Ovarian Follicle - physiology Time Factors Vertebrates: reproduction |
| title | Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro |
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