Affinity–reversed-phase liquid chromatography assay to quantitate recombinant antibodies and antibody fragments in fermentation broth

An automated dual-column liquid chromatography assay comprised of affinity and reversed-phase separations that quantifies the majority of antibody-related protein species found in crude cell extracts of recombinant origin is described. Although potentially applicable to any antibody preparation, we...

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Veröffentlicht in:	Journal of Chromatography A 2001-08, Vol.927 (1), p.61-76
Hauptverfasser:	Battersby, John E, Snedecor, Brad, Chen, Christina, Champion, Kathleen M, Riddle, Lavon, Vanderlaan, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Antibodies - analysis Biological and medical sciences Biotechnology Chromatography, Affinity - methods Electrophoresis, Gel, Two-Dimensional Fermentation Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Immunoglobulin Fragments - analysis Industrial applications and implications. Economical aspects Mass Spectrometry Monoclonal antibodies Production of active biomolecules Recombinant Proteins - analysis
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