Characterization of G-protein Signaling in Ventricular Myocytes From the Adult Mouse Heart: Differences From the Rat

We have developed a high yield technique for isolating ventricular myocytes from adult mouse hearts. This collagenase-trypsin procedure yields 3–6×106cells/heart. The cells are rod-shaped, roughly 20μM ×100 μM and Ca++tolerant, with viability of 65–80%. Binding studies with [125I]ICYP demonstrate th...

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Veröffentlicht in:	Journal of molecular and cellular cardiology 2000-07, Vol.32 (7), p.1211-1221
Hauptverfasser:	Hilal-Dandan, Randa, Kanter, Joan R, Brunton, Laurence L
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylate Cyclase Toxin Adenylyl Cyclases - metabolism Adrenergic beta-Agonists - pharmacology Animals Cell Culture Techniques - methods Cells, Cultured Cholinergic Agonists - pharmacology Cyclic AMP - metabolism Dose-Response Relationship, Drug GTP-Binding Proteins - metabolism Heart - physiology Inositol Phosphates - biosynthesis Isoproterenol - pharmacology Male Mice Myocardium - cytology Myocardium - metabolism Pertussis Toxin Phosphoinositide metabolism Protein Binding Rats Receptors, Adrenergic, beta - metabolism Signal Transduction Transgenic mice β -adrenergic receptors α1-adrenergic receptors Virulence Factors, Bordetella - pharmacology
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