Development of Binding Assays in Microfabricated Picoliter Vials:  An Assay for Biotin

A homogeneous binding assay for the detection of biotin in picoliter vials was developed using the photoprotein aequorin as the label. The binding assay was based on the competition of free biotin with biotinylated aequorin (AEQ-biotin) for avidin. A sequential protocol was used, and modification of...

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Veröffentlicht in:	Analytical chemistry (Washington) 2000-06, Vol.72 (11), p.2590-2594
Hauptverfasser:	Grosvenor, Anne L, Feltus, Agatha, Conover, Richard C, Daunert, Sylvia, Anderson, Kimberly W
Format:	Artikel
Sprache:	eng
Schlagworte:	Aequorin - chemistry Analysis Avidin - chemistry Biotin - analysis Biotin - chemistry Calibration Cells Microchemistry Product development Sensors
Online-Zugang:	Volltext
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