Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization
Chronic infection by Pseudomonas aeruginosa (PA) in patients with cystic fibrosis (CF) is preceded by a period of colonization and acute infection. Early aggressive antibiotic treatment of initial colonisation may prevent or at least delay chronic pulmonary infection. We initiated treatment with a c...
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| Veröffentlicht in: | Pediatric pulmonology 2001-10, Vol.32 (4), p.288-292 |
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| container_title | Pediatric pulmonology |
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| creator | Munck, Anne Bonacorsi, Stéphane Mariani-Kurkdjian, Patricia Lebourgeois, Muriel Gérardin, Michèle Brahimi, Naïma Navarro, Jean Bingen, Edouard |
| description | Chronic infection by Pseudomonas aeruginosa (PA) in patients with cystic fibrosis (CF) is preceded by a period of colonization and acute infection. Early aggressive antibiotic treatment of initial colonisation may prevent or at least delay chronic pulmonary infection. We initiated treatment with a combination of IV β‐lactam tobramycin, followed by nebulized colistin when PA was first isolated from patients with CF. Subsequent serial PA isolates obtained from these colonized CF patients were characterized by means of molecular methods to determine whether they were genetically related to the initial strain.
Initial colonization was eradicated in all 19 patients. All patients reacquired PA within 3–25 months during the 3 years of follow‐up. Fourteen patients acquired a new PA strain with a distinct genotypic profile, suggesting a new source of contamination. Five patients had two PA isolates with identical genotypes, suggesting either previous undetected respiratory tract colonization or a persistent environmental source of contamination. Pediatr Pulmonol. 2001; 32:288–292. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ppul.1121 |
| format | Article |
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Initial colonization was eradicated in all 19 patients. All patients reacquired PA within 3–25 months during the 3 years of follow‐up. Fourteen patients acquired a new PA strain with a distinct genotypic profile, suggesting a new source of contamination. Five patients had two PA isolates with identical genotypes, suggesting either previous undetected respiratory tract colonization or a persistent environmental source of contamination. Pediatr Pulmonol. 2001; 32:288–292. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 8755-6863</identifier><identifier>EISSN: 1099-0496</identifier><identifier>DOI: 10.1002/ppul.1121</identifier><identifier>PMID: 11568989</identifier><identifier>CODEN: PEPUES</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Administration, Inhalation ; Administration, Oral ; bacterial genotyping ; Biological and medical sciences ; Ceftazidime - administration & dosage ; Child ; Child, Preschool ; Chronic Disease ; Colistin - administration & dosage ; Colony Count, Microbial ; cystic fibrosis ; Cystic Fibrosis - drug therapy ; Cystic Fibrosis - microbiology ; DNA, Bacterial - analysis ; Drug Therapy, Combination - administration & dosage ; Electrophoresis, Gel, Pulsed-Field ; Female ; Genotype ; Humans ; Imipenem - administration & dosage ; Infant ; Male ; Medical sciences ; molecular analysis ; Pneumology ; Pneumonia, Bacterial - diagnosis ; Pneumonia, Bacterial - drug therapy ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - genetics ; Pseudomonas Infections - diagnosis ; Pseudomonas Infections - drug therapy ; Respiratory system : syndromes and miscellaneous diseases ; Risk Assessment ; Tobramycin - administration & dosage</subject><ispartof>Pediatric pulmonology, 2001-10, Vol.32 (4), p.288-292</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4141-d32d2329fd6897350f4862db1e0ffc4d64733ad83d179761ba295837aff46573</citedby><cites>FETCH-LOGICAL-c4141-d32d2329fd6897350f4862db1e0ffc4d64733ad83d179761ba295837aff46573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fppul.1121$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fppul.1121$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14090273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11568989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Munck, Anne</creatorcontrib><creatorcontrib>Bonacorsi, Stéphane</creatorcontrib><creatorcontrib>Mariani-Kurkdjian, Patricia</creatorcontrib><creatorcontrib>Lebourgeois, Muriel</creatorcontrib><creatorcontrib>Gérardin, Michèle</creatorcontrib><creatorcontrib>Brahimi, Naïma</creatorcontrib><creatorcontrib>Navarro, Jean</creatorcontrib><creatorcontrib>Bingen, Edouard</creatorcontrib><title>Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization</title><title>Pediatric pulmonology</title><addtitle>Pediatr. Pulmonol</addtitle><description>Chronic infection by Pseudomonas aeruginosa (PA) in patients with cystic fibrosis (CF) is preceded by a period of colonization and acute infection. Early aggressive antibiotic treatment of initial colonisation may prevent or at least delay chronic pulmonary infection. We initiated treatment with a combination of IV β‐lactam tobramycin, followed by nebulized colistin when PA was first isolated from patients with CF. Subsequent serial PA isolates obtained from these colonized CF patients were characterized by means of molecular methods to determine whether they were genetically related to the initial strain.
Initial colonization was eradicated in all 19 patients. All patients reacquired PA within 3–25 months during the 3 years of follow‐up. Fourteen patients acquired a new PA strain with a distinct genotypic profile, suggesting a new source of contamination. Five patients had two PA isolates with identical genotypes, suggesting either previous undetected respiratory tract colonization or a persistent environmental source of contamination. Pediatr Pulmonol. 2001; 32:288–292. © 2001 Wiley‐Liss, Inc.</description><subject>Administration, Inhalation</subject><subject>Administration, Oral</subject><subject>bacterial genotyping</subject><subject>Biological and medical sciences</subject><subject>Ceftazidime - administration & dosage</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Chronic Disease</subject><subject>Colistin - administration & dosage</subject><subject>Colony Count, Microbial</subject><subject>cystic fibrosis</subject><subject>Cystic Fibrosis - drug therapy</subject><subject>Cystic Fibrosis - microbiology</subject><subject>DNA, Bacterial - analysis</subject><subject>Drug Therapy, Combination - administration & dosage</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Imipenem - administration & dosage</subject><subject>Infant</subject><subject>Male</subject><subject>Medical sciences</subject><subject>molecular analysis</subject><subject>Pneumology</subject><subject>Pneumonia, Bacterial - diagnosis</subject><subject>Pneumonia, Bacterial - drug therapy</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas Infections - diagnosis</subject><subject>Pseudomonas Infections - drug therapy</subject><subject>Respiratory system : syndromes and miscellaneous diseases</subject><subject>Risk Assessment</subject><subject>Tobramycin - administration & dosage</subject><issn>8755-6863</issn><issn>1099-0496</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc2O0zAUhS0EYkphwQsgb0BikRk7duJ4yVRQEBVEaACJjeX4hzGkdrAdhvIavDCuGjErVl74O-fcew8AjzE6xwjVF9M0j-cY1_gOWGHEeYUob--CVceapmq7lpyBByl9Q6j8cXwfnGHctB3v-Ar82Rof8mFyCqprGaXKJrrfMrvgYbCwT2bWYR-8TFCaOH91PiQJU47S-QSjUeGniUZDG8MeTkVnfE7wxuVrqA4pF1vrhhiSK3pbvKHzLjs5Quk1TPOQzI-5SKAKY_BL8ENwz8oxmUfLuwZXr15ebV5Xu_fbN5sXu0pRTHGlSa1rUnOryy6MNMjSrq31gA2yVlHdUkaI1B3RmHHW4kHWvOkIk9bStmFkDZ6dbKcYyhApi71Lyoyj9CbMSTCMOWKUFvD5CVRlkRSNFVN0exkPAiNxLEAcCxDHAgr7ZDGdh73Rt-Ry8QI8XQCZlBxtlF65dMtRxFFdBl-DixN340Zz-H-i6PuPuyW6OilcyubXP4WM30XLCGvE53dbsfnU95dfPuzEW_IX1U6xJA</recordid><startdate>200110</startdate><enddate>200110</enddate><creator>Munck, Anne</creator><creator>Bonacorsi, Stéphane</creator><creator>Mariani-Kurkdjian, Patricia</creator><creator>Lebourgeois, Muriel</creator><creator>Gérardin, Michèle</creator><creator>Brahimi, Naïma</creator><creator>Navarro, Jean</creator><creator>Bingen, Edouard</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200110</creationdate><title>Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization</title><author>Munck, Anne ; Bonacorsi, Stéphane ; Mariani-Kurkdjian, Patricia ; Lebourgeois, Muriel ; Gérardin, Michèle ; Brahimi, Naïma ; Navarro, Jean ; Bingen, Edouard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4141-d32d2329fd6897350f4862db1e0ffc4d64733ad83d179761ba295837aff46573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Administration, Inhalation</topic><topic>Administration, Oral</topic><topic>bacterial genotyping</topic><topic>Biological and medical sciences</topic><topic>Ceftazidime - administration & dosage</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Chronic Disease</topic><topic>Colistin - administration & dosage</topic><topic>Colony Count, Microbial</topic><topic>cystic fibrosis</topic><topic>Cystic Fibrosis - drug therapy</topic><topic>Cystic Fibrosis - microbiology</topic><topic>DNA, Bacterial - analysis</topic><topic>Drug Therapy, Combination - administration & dosage</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Imipenem - administration & dosage</topic><topic>Infant</topic><topic>Male</topic><topic>Medical sciences</topic><topic>molecular analysis</topic><topic>Pneumology</topic><topic>Pneumonia, Bacterial - diagnosis</topic><topic>Pneumonia, Bacterial - drug therapy</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas Infections - diagnosis</topic><topic>Pseudomonas Infections - drug therapy</topic><topic>Respiratory system : syndromes and miscellaneous diseases</topic><topic>Risk Assessment</topic><topic>Tobramycin - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munck, Anne</creatorcontrib><creatorcontrib>Bonacorsi, Stéphane</creatorcontrib><creatorcontrib>Mariani-Kurkdjian, Patricia</creatorcontrib><creatorcontrib>Lebourgeois, Muriel</creatorcontrib><creatorcontrib>Gérardin, Michèle</creatorcontrib><creatorcontrib>Brahimi, Naïma</creatorcontrib><creatorcontrib>Navarro, Jean</creatorcontrib><creatorcontrib>Bingen, Edouard</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric pulmonology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munck, Anne</au><au>Bonacorsi, Stéphane</au><au>Mariani-Kurkdjian, Patricia</au><au>Lebourgeois, Muriel</au><au>Gérardin, Michèle</au><au>Brahimi, Naïma</au><au>Navarro, Jean</au><au>Bingen, Edouard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization</atitle><jtitle>Pediatric pulmonology</jtitle><addtitle>Pediatr. Pulmonol</addtitle><date>2001-10</date><risdate>2001</risdate><volume>32</volume><issue>4</issue><spage>288</spage><epage>292</epage><pages>288-292</pages><issn>8755-6863</issn><eissn>1099-0496</eissn><coden>PEPUES</coden><abstract>Chronic infection by Pseudomonas aeruginosa (PA) in patients with cystic fibrosis (CF) is preceded by a period of colonization and acute infection. Early aggressive antibiotic treatment of initial colonisation may prevent or at least delay chronic pulmonary infection. We initiated treatment with a combination of IV β‐lactam tobramycin, followed by nebulized colistin when PA was first isolated from patients with CF. Subsequent serial PA isolates obtained from these colonized CF patients were characterized by means of molecular methods to determine whether they were genetically related to the initial strain.
Initial colonization was eradicated in all 19 patients. All patients reacquired PA within 3–25 months during the 3 years of follow‐up. Fourteen patients acquired a new PA strain with a distinct genotypic profile, suggesting a new source of contamination. Five patients had two PA isolates with identical genotypes, suggesting either previous undetected respiratory tract colonization or a persistent environmental source of contamination. Pediatr Pulmonol. 2001; 32:288–292. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11568989</pmid><doi>10.1002/ppul.1121</doi><tpages>5</tpages></addata></record> |
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| ispartof | Pediatric pulmonology, 2001-10, Vol.32 (4), p.288-292 |
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| subjects | Administration, Inhalation Administration, Oral bacterial genotyping Biological and medical sciences Ceftazidime - administration & dosage Child Child, Preschool Chronic Disease Colistin - administration & dosage Colony Count, Microbial cystic fibrosis Cystic Fibrosis - drug therapy Cystic Fibrosis - microbiology DNA, Bacterial - analysis Drug Therapy, Combination - administration & dosage Electrophoresis, Gel, Pulsed-Field Female Genotype Humans Imipenem - administration & dosage Infant Male Medical sciences molecular analysis Pneumology Pneumonia, Bacterial - diagnosis Pneumonia, Bacterial - drug therapy Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas Infections - diagnosis Pseudomonas Infections - drug therapy Respiratory system : syndromes and miscellaneous diseases Risk Assessment Tobramycin - administration & dosage |
| title | Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization |
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