Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil
Summary An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were ly...
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| Veröffentlicht in: | Journal of veterinary medicine. Series B 2000-05, Vol.47 (4), p.241-248 |
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| container_title | Journal of veterinary medicine. Series B |
| container_volume | 47 |
| creator | Braz jr, C.J. Ribeiro, M.F.B. Lima, J.D. Passos, L.M.F. |
| description | Summary
An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were lysed with 0.9% NH4Cl and stored at ‐ 20°C. Typical anaplasmal structures were seen by electron microscopy in the antigenic preparations containing the erythrocytes that had been stored with DMSO. The performance of the ELISA test was evaluated by testing 298 positive serum samples collected from immunized cattle, 39 negative serum samples collected from cattle imported from areas free of A. marginale and 50 samples collected from cattle naturally infected in the field. The test gave a specificity of 94.87% and a sensitivity of 100%. |
| doi_str_mv | 10.1046/j.1439-0450.2000.00342.x |
| format | Article |
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An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were lysed with 0.9% NH4Cl and stored at ‐ 20°C. Typical anaplasmal structures were seen by electron microscopy in the antigenic preparations containing the erythrocytes that had been stored with DMSO. The performance of the ELISA test was evaluated by testing 298 positive serum samples collected from immunized cattle, 39 negative serum samples collected from cattle imported from areas free of A. marginale and 50 samples collected from cattle naturally infected in the field. The test gave a specificity of 94.87% and a sensitivity of 100%.</description><identifier>ISSN: 0931-1793</identifier><identifier>EISSN: 1439-0450</identifier><identifier>DOI: 10.1046/j.1439-0450.2000.00342.x</identifier><identifier>PMID: 10861192</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science, Ltd</publisher><subject>Anaplasma - immunology ; Anaplasma - isolation & purification ; Anaplasma marginale ; Anaplasmosis - diagnosis ; Anaplasmosis - immunology ; Anaplasmosis - microbiology ; Animals ; Animals, Newborn ; Antibodies, Bacterial - blood ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; Enzyme-Linked Immunosorbent Assay - veterinary ; Sensitivity and Specificity</subject><ispartof>Journal of veterinary medicine. Series B, 2000-05, Vol.47 (4), p.241-248</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4332-b54449b966e9748d366c431988c517cbd52605cd27edd08dc549a5be63e29e4d3</citedby><cites>FETCH-LOGICAL-c4332-b54449b966e9748d366c431988c517cbd52605cd27edd08dc549a5be63e29e4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1439-0450.2000.00342.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1439-0450.2000.00342.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10861192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Braz jr, C.J.</creatorcontrib><creatorcontrib>Ribeiro, M.F.B.</creatorcontrib><creatorcontrib>Lima, J.D.</creatorcontrib><creatorcontrib>Passos, L.M.F.</creatorcontrib><title>Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil</title><title>Journal of veterinary medicine. Series B</title><addtitle>J Vet Med B Infect Dis Vet Public Health</addtitle><description>Summary
An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were lysed with 0.9% NH4Cl and stored at ‐ 20°C. Typical anaplasmal structures were seen by electron microscopy in the antigenic preparations containing the erythrocytes that had been stored with DMSO. The performance of the ELISA test was evaluated by testing 298 positive serum samples collected from immunized cattle, 39 negative serum samples collected from cattle imported from areas free of A. marginale and 50 samples collected from cattle naturally infected in the field. The test gave a specificity of 94.87% and a sensitivity of 100%.</description><subject>Anaplasma - immunology</subject><subject>Anaplasma - isolation & purification</subject><subject>Anaplasma marginale</subject><subject>Anaplasmosis - diagnosis</subject><subject>Anaplasmosis - immunology</subject><subject>Anaplasmosis - microbiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antibodies, Bacterial - blood</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - microbiology</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Sensitivity and Specificity</subject><issn>0931-1793</issn><issn>1439-0450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EoqOhfwF5xS7Bb8cSm6EtbcUIigpUYmM59p3KJY8hzsAMvx6nqSp2sPKVz3eufe9BCFNSUiLU67uSCm4KIiQpGSGkJIQLVu6foMWj8BQtiOG0oNrwI3ScUqwzyZgkxDxHR5RUilLDFuj2FH5C029b6Ebcb7Dr8Nn68nqF0yGN0OJNP-BTGMGPse8mYNWNsVh1btu41DrcuuE2dq6Be6HuQ4SEY4e9G8d8mau3g_sdmxfo2cY1CY4fziX68u7s88lFsf54fnmyWhdecM6KWgohTG2UAqNFFbhSWaCmqryk2tdBMkWkD0xDCKQKXgrjZA2KAzMgAl-iV3Pf7dD_2EEabRuTh6ZxHfS7ZHUemyim_wlSrYhh-VNLVM2gH_qUBtjY7RDz2AdLiZ0CsXd22rud9m6nQOx9IHafrS8f3tjVLYS_jPP6M_BmBn7FBg7_3dh-u7rIRbYXsz3mrPaPdjd8t0pzLe3Nh3N7U1Xvr75-uraM_wG8zKc2</recordid><startdate>200005</startdate><enddate>200005</enddate><creator>Braz jr, C.J.</creator><creator>Ribeiro, M.F.B.</creator><creator>Lima, J.D.</creator><creator>Passos, L.M.F.</creator><general>Blackwell Science, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200005</creationdate><title>Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil</title><author>Braz jr, C.J. ; Ribeiro, M.F.B. ; Lima, J.D. ; Passos, L.M.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4332-b54449b966e9748d366c431988c517cbd52605cd27edd08dc549a5be63e29e4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Anaplasma - immunology</topic><topic>Anaplasma - isolation & purification</topic><topic>Anaplasma marginale</topic><topic>Anaplasmosis - diagnosis</topic><topic>Anaplasmosis - immunology</topic><topic>Anaplasmosis - microbiology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antibodies, Bacterial - blood</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - microbiology</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Sensitivity and Specificity</topic><toplevel>online_resources</toplevel><creatorcontrib>Braz jr, C.J.</creatorcontrib><creatorcontrib>Ribeiro, M.F.B.</creatorcontrib><creatorcontrib>Lima, J.D.</creatorcontrib><creatorcontrib>Passos, L.M.F.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary medicine. Series B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Braz jr, C.J.</au><au>Ribeiro, M.F.B.</au><au>Lima, J.D.</au><au>Passos, L.M.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil</atitle><jtitle>Journal of veterinary medicine. Series B</jtitle><addtitle>J Vet Med B Infect Dis Vet Public Health</addtitle><date>2000-05</date><risdate>2000</risdate><volume>47</volume><issue>4</issue><spage>241</spage><epage>248</epage><pages>241-248</pages><issn>0931-1793</issn><eissn>1439-0450</eissn><abstract>Summary
An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were lysed with 0.9% NH4Cl and stored at ‐ 20°C. Typical anaplasmal structures were seen by electron microscopy in the antigenic preparations containing the erythrocytes that had been stored with DMSO. The performance of the ELISA test was evaluated by testing 298 positive serum samples collected from immunized cattle, 39 negative serum samples collected from cattle imported from areas free of A. marginale and 50 samples collected from cattle naturally infected in the field. The test gave a specificity of 94.87% and a sensitivity of 100%.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science, Ltd</pub><pmid>10861192</pmid><doi>10.1046/j.1439-0450.2000.00342.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of veterinary medicine. Series B, 2000-05, Vol.47 (4), p.241-248 |
| issn | 0931-1793 1439-0450 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anaplasma - immunology Anaplasma - isolation & purification Anaplasma marginale Anaplasmosis - diagnosis Anaplasmosis - immunology Anaplasmosis - microbiology Animals Animals, Newborn Antibodies, Bacterial - blood Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology Enzyme-Linked Immunosorbent Assay - veterinary Sensitivity and Specificity |
| title | Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil |
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