Mean DNA Bend Angle and Distribution of DNA Bend Angles in the CAP-DNA Complex in Solution

In order to define the mean DNA bend angle and distribution of DNA bend angles in the catabolite activator protein (CAP)-DNA complex in solution under standard transcription initiation conditions, we have performed nanosecond time-resolved fluorescence measurements quantifying energy transfer betwee...

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Veröffentlicht in:	Journal of molecular biology 2001-09, Vol.312 (3), p.453-468
Hauptverfasser:	Kapanidis, Achillefs N., Ebright, Yon W., Ludescher, Richard D., Chan, Shirley, Ebright, Richard H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Buffers cAMP receptor protein (CRP) catabolite activator protein (CAP) Computer Simulation Cyclic AMP Receptor Protein - chemistry Cyclic AMP Receptor Protein - metabolism DNA - chemistry DNA - genetics DNA - metabolism DNA bending DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Energy Transfer Fluorescence Fluorescence Polarization fluorescence resonance energy transfer Models, Molecular Nucleic Acid Conformation Protein Binding Protein Conformation protein-induced DNA bending Response Elements - genetics Solutions Transcription, Genetic transcriptional activation
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