Number and Sites of rDNA Loci of Guizotia Abyssinica (L. f.) Cass. as Determined by Fluorescence in Situ Hybridization

Fluorescence in situ hybridization (FISH) was employed on somatic chromosome preparations of Guizotia abyssinica using a DNA probe of the 18S‐5.8S‐26S rRNA genes including the transcribed and non‐transcribed spacer sequences. A maximum of six major and two minor signal sites of rDNA was observed. Ho...

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Veröffentlicht in:Hereditas 2000-02, Vol.132 (1), p.63-65
Hauptverfasser: Dagne, Kifle, Cheng, Bifang, Heneen, Waheeb K.
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description Fluorescence in situ hybridization (FISH) was employed on somatic chromosome preparations of Guizotia abyssinica using a DNA probe of the 18S‐5.8S‐26S rRNA genes including the transcribed and non‐transcribed spacer sequences. A maximum of six major and two minor signal sites of rDNA was observed. However, the minor signals were not persistently detected. The positions of the FISH signals coincided with the sites of the nucleolar organizer regions and their adjacent C‐banded heterochromatin when present.
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subjects Asteraceae - genetics
Chromatin - genetics
Chromosomes
DNA, Ribosomal - genetics
Genes, Plant
Guizotia abyssinica
In Situ Hybridization, Fluorescence
RNA, Satellite
title Number and Sites of rDNA Loci of Guizotia Abyssinica (L. f.) Cass. as Determined by Fluorescence in Situ Hybridization
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