Kinetics of a Putative Hypoxic Tracer, 99mTc-HL91, in Normoxic, Hypoxic, Ischemic, and Stunned Myocardium
99mTc-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime (HL91) was developed as a putative hypoxic reagent. This study focused on the myocardial kinetics of 99mTc-HL91 in various oxygen levels and perfusion states. The time-activity curve of 99mTc-HL91 was measured in isolated perfused rat h...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 2000-06, Vol.41 (6), p.1102-1107 |
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| container_title | The Journal of nuclear medicine (1978) |
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| creator | Imahashi, Kenichi Morishita, Kenichi Kusuoka, Hideo Yamamichi, Yoshihiro Hasegawa, Shinji Hashimoto, Katsuji Shirakami, Yoshifumi Kato-Azuma, Makoto Nishimura, Tsunehiko |
| description | 99mTc-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime (HL91) was developed as a putative hypoxic reagent. This study focused on the myocardial kinetics of 99mTc-HL91 in various oxygen levels and perfusion states.
The time-activity curve of 99mTc-HL91 was measured in isolated perfused rat heart after the bolus infusion.
99mTc-HL91 was cleared quickly from normoxic hearts, and retention at 30 min after injection was 0.18 +/- 0.02 percentage injected dose per gram of wet weight (mean +/- SE; n = 6). When the concentration of oxygen bubbling through the perfusate was reduced from 100% to 50%, 20%, 5%, and 0%, retention of 99mTc-HL91 increased to 0.47 +/- 0.03 (n = 5), 0.48 +/- 0.03 (n = 5), 0.71 +/- 0.01 (n = 5), and 0.70 +/- 0.02 (n = 5), respectively (P < 0.05). Compartment analysis revealed that the trapping mechanism, which was dependent on tissue oxygen concentration, determined the retention rate. Although not retained in stunned myocardium (0.17 +/- 0.02, n = 5; P = not significant), 99mTc-HL91 was significantly retained when injected before ischemia (1.06 +/- 0.06, n = 5; P < 0.05).
These results indicate that retention of 99mTc-HL91 correlates well with oxygen level in the perfusate, suggesting that the agent may be a useful marker of the severity of myocardial hypoxia. |
| format | Article |
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The time-activity curve of 99mTc-HL91 was measured in isolated perfused rat heart after the bolus infusion.
99mTc-HL91 was cleared quickly from normoxic hearts, and retention at 30 min after injection was 0.18 +/- 0.02 percentage injected dose per gram of wet weight (mean +/- SE; n = 6). When the concentration of oxygen bubbling through the perfusate was reduced from 100% to 50%, 20%, 5%, and 0%, retention of 99mTc-HL91 increased to 0.47 +/- 0.03 (n = 5), 0.48 +/- 0.03 (n = 5), 0.71 +/- 0.01 (n = 5), and 0.70 +/- 0.02 (n = 5), respectively (P < 0.05). Compartment analysis revealed that the trapping mechanism, which was dependent on tissue oxygen concentration, determined the retention rate. Although not retained in stunned myocardium (0.17 +/- 0.02, n = 5; P = not significant), 99mTc-HL91 was significantly retained when injected before ischemia (1.06 +/- 0.06, n = 5; P < 0.05).
These results indicate that retention of 99mTc-HL91 correlates well with oxygen level in the perfusate, suggesting that the agent may be a useful marker of the severity of myocardial hypoxia.</description><identifier>ISSN: 0161-5505</identifier><identifier>EISSN: 1535-5667</identifier><identifier>PMID: 10855642</identifier><identifier>CODEN: JNMEAQ</identifier><language>eng</language><publisher>United States: Soc Nuclear Med</publisher><subject>Animals ; Heart - diagnostic imaging ; Hypoxia - diagnostic imaging ; Hypoxia - metabolism ; In Vitro Techniques ; Male ; Myocardial Ischemia - diagnostic imaging ; Myocardial Stunning - diagnostic imaging ; Myocardium - metabolism ; Organotechnetium Compounds - pharmacokinetics ; Oximes - pharmacokinetics ; Oxygen Consumption ; Radionuclide Imaging ; Rats ; Rats, Sprague-Dawley</subject><ispartof>The Journal of nuclear medicine (1978), 2000-06, Vol.41 (6), p.1102-1107</ispartof><rights>Copyright Society of Nuclear Medicine Jun 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10855642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imahashi, Kenichi</creatorcontrib><creatorcontrib>Morishita, Kenichi</creatorcontrib><creatorcontrib>Kusuoka, Hideo</creatorcontrib><creatorcontrib>Yamamichi, Yoshihiro</creatorcontrib><creatorcontrib>Hasegawa, Shinji</creatorcontrib><creatorcontrib>Hashimoto, Katsuji</creatorcontrib><creatorcontrib>Shirakami, Yoshifumi</creatorcontrib><creatorcontrib>Kato-Azuma, Makoto</creatorcontrib><creatorcontrib>Nishimura, Tsunehiko</creatorcontrib><title>Kinetics of a Putative Hypoxic Tracer, 99mTc-HL91, in Normoxic, Hypoxic, Ischemic, and Stunned Myocardium</title><title>The Journal of nuclear medicine (1978)</title><addtitle>J Nucl Med</addtitle><description>99mTc-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime (HL91) was developed as a putative hypoxic reagent. This study focused on the myocardial kinetics of 99mTc-HL91 in various oxygen levels and perfusion states.
The time-activity curve of 99mTc-HL91 was measured in isolated perfused rat heart after the bolus infusion.
99mTc-HL91 was cleared quickly from normoxic hearts, and retention at 30 min after injection was 0.18 +/- 0.02 percentage injected dose per gram of wet weight (mean +/- SE; n = 6). When the concentration of oxygen bubbling through the perfusate was reduced from 100% to 50%, 20%, 5%, and 0%, retention of 99mTc-HL91 increased to 0.47 +/- 0.03 (n = 5), 0.48 +/- 0.03 (n = 5), 0.71 +/- 0.01 (n = 5), and 0.70 +/- 0.02 (n = 5), respectively (P < 0.05). Compartment analysis revealed that the trapping mechanism, which was dependent on tissue oxygen concentration, determined the retention rate. Although not retained in stunned myocardium (0.17 +/- 0.02, n = 5; P = not significant), 99mTc-HL91 was significantly retained when injected before ischemia (1.06 +/- 0.06, n = 5; P < 0.05).
These results indicate that retention of 99mTc-HL91 correlates well with oxygen level in the perfusate, suggesting that the agent may be a useful marker of the severity of myocardial hypoxia.</description><subject>Animals</subject><subject>Heart - diagnostic imaging</subject><subject>Hypoxia - diagnostic imaging</subject><subject>Hypoxia - metabolism</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Myocardial Ischemia - diagnostic imaging</subject><subject>Myocardial Stunning - diagnostic imaging</subject><subject>Myocardium - metabolism</subject><subject>Organotechnetium Compounds - pharmacokinetics</subject><subject>Oximes - pharmacokinetics</subject><subject>Oxygen Consumption</subject><subject>Radionuclide Imaging</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0F1LwzAUBuAiipsff0GCF3rTQtIsaXopQ91wfoDzuqTJqc1o05k06v69HdtuvDoH3ofDyzmKxoRRljDOs-NojAknCWOYjaIz71cYYy6EOI1GBAvG-CQdR-bJWOiN8qirkERvoZe9-QY026y7X6PQ0kkFLkZ53i5VMlvkJEbGopfOtds8PsAYzb2qod1u0mr03gdrQaPnTaek0ya0F9FJJRsPl_t5Hn083C-ns2Tx-jif3i2SmhCaJhQqXimZZawSGEslcl4xWeoUNKMV0FzpVJdZpkolhZgIxqkiuqRESSY0VPQ8utndXbvuK4Dvi9Z4BU0jLXTBFxkhgouMDvD6H1x1wdmhW5GSnOR0kAO62qNQtqCLtTOtdJvi8MEB3O5AbT7rH-OgsEE1IN1Wr2w7IQUvCMEp_QPfvHpx</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Imahashi, Kenichi</creator><creator>Morishita, Kenichi</creator><creator>Kusuoka, Hideo</creator><creator>Yamamichi, Yoshihiro</creator><creator>Hasegawa, Shinji</creator><creator>Hashimoto, Katsuji</creator><creator>Shirakami, Yoshifumi</creator><creator>Kato-Azuma, Makoto</creator><creator>Nishimura, Tsunehiko</creator><general>Soc Nuclear Med</general><general>Society of Nuclear Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>4T-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>M7Z</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Kinetics of a Putative Hypoxic Tracer, 99mTc-HL91, in Normoxic, Hypoxic, Ischemic, and Stunned Myocardium</title><author>Imahashi, Kenichi ; Morishita, Kenichi ; Kusuoka, Hideo ; Yamamichi, Yoshihiro ; Hasegawa, Shinji ; Hashimoto, Katsuji ; Shirakami, Yoshifumi ; Kato-Azuma, Makoto ; Nishimura, Tsunehiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h1132-3ef6fca775f800ac896f5abd2ed53fe39cd2db77cbca8848563c1db31ca58def3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Heart - diagnostic imaging</topic><topic>Hypoxia - diagnostic imaging</topic><topic>Hypoxia - metabolism</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Myocardial Ischemia - diagnostic imaging</topic><topic>Myocardial Stunning - diagnostic imaging</topic><topic>Myocardium - metabolism</topic><topic>Organotechnetium Compounds - pharmacokinetics</topic><topic>Oximes - pharmacokinetics</topic><topic>Oxygen Consumption</topic><topic>Radionuclide Imaging</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imahashi, Kenichi</creatorcontrib><creatorcontrib>Morishita, Kenichi</creatorcontrib><creatorcontrib>Kusuoka, Hideo</creatorcontrib><creatorcontrib>Yamamichi, Yoshihiro</creatorcontrib><creatorcontrib>Hasegawa, Shinji</creatorcontrib><creatorcontrib>Hashimoto, Katsuji</creatorcontrib><creatorcontrib>Shirakami, Yoshifumi</creatorcontrib><creatorcontrib>Kato-Azuma, Makoto</creatorcontrib><creatorcontrib>Nishimura, Tsunehiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imahashi, Kenichi</au><au>Morishita, Kenichi</au><au>Kusuoka, Hideo</au><au>Yamamichi, Yoshihiro</au><au>Hasegawa, Shinji</au><au>Hashimoto, Katsuji</au><au>Shirakami, Yoshifumi</au><au>Kato-Azuma, Makoto</au><au>Nishimura, Tsunehiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of a Putative Hypoxic Tracer, 99mTc-HL91, in Normoxic, Hypoxic, Ischemic, and Stunned Myocardium</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><addtitle>J Nucl Med</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>41</volume><issue>6</issue><spage>1102</spage><epage>1107</epage><pages>1102-1107</pages><issn>0161-5505</issn><eissn>1535-5667</eissn><coden>JNMEAQ</coden><abstract>99mTc-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime (HL91) was developed as a putative hypoxic reagent. This study focused on the myocardial kinetics of 99mTc-HL91 in various oxygen levels and perfusion states.
The time-activity curve of 99mTc-HL91 was measured in isolated perfused rat heart after the bolus infusion.
99mTc-HL91 was cleared quickly from normoxic hearts, and retention at 30 min after injection was 0.18 +/- 0.02 percentage injected dose per gram of wet weight (mean +/- SE; n = 6). When the concentration of oxygen bubbling through the perfusate was reduced from 100% to 50%, 20%, 5%, and 0%, retention of 99mTc-HL91 increased to 0.47 +/- 0.03 (n = 5), 0.48 +/- 0.03 (n = 5), 0.71 +/- 0.01 (n = 5), and 0.70 +/- 0.02 (n = 5), respectively (P < 0.05). Compartment analysis revealed that the trapping mechanism, which was dependent on tissue oxygen concentration, determined the retention rate. Although not retained in stunned myocardium (0.17 +/- 0.02, n = 5; P = not significant), 99mTc-HL91 was significantly retained when injected before ischemia (1.06 +/- 0.06, n = 5; P < 0.05).
These results indicate that retention of 99mTc-HL91 correlates well with oxygen level in the perfusate, suggesting that the agent may be a useful marker of the severity of myocardial hypoxia.</abstract><cop>United States</cop><pub>Soc Nuclear Med</pub><pmid>10855642</pmid><tpages>6</tpages></addata></record> |
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| subjects | Animals Heart - diagnostic imaging Hypoxia - diagnostic imaging Hypoxia - metabolism In Vitro Techniques Male Myocardial Ischemia - diagnostic imaging Myocardial Stunning - diagnostic imaging Myocardium - metabolism Organotechnetium Compounds - pharmacokinetics Oximes - pharmacokinetics Oxygen Consumption Radionuclide Imaging Rats Rats, Sprague-Dawley |
| title | Kinetics of a Putative Hypoxic Tracer, 99mTc-HL91, in Normoxic, Hypoxic, Ischemic, and Stunned Myocardium |
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