Placental Expression and Chromosomal Localization of the Human Gcm 1 Gene
Although gcm was first recognized for its role in specifying glial cell fate in Drosophila melanogaster, its mammalian counterparts are expressed predominantly in non-neural tissues. Here we demonstrate expression of the mouse and human GCM 1 proteins in placenta. We have prepared a highly specific...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2000-07, Vol.48 (7), p.915-922 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Nait-Oumesmar, Brahim Copperman, Alan B Lazzarini, Robert A |
| description | Although gcm was first recognized for its role in specifying glial cell fate in Drosophila melanogaster, its mammalian counterparts are expressed predominantly in non-neural tissues. Here we demonstrate expression of the mouse and human GCM 1 proteins in placenta. We have prepared a highly specific antibody that recognizes the GCM 1 protein and have used it to assess the temporal and spatial expression profile of the protein. In both mouse and human placenta, the protein is associated with cells that are involved with exchange between maternal and fetal blood supplies: the labyrinthine cells of the mouse placenta and the syncytio- and cytotrophoblasts of the human placenta. Using the full-length hGcm 1 cDNA as a probe, we have mapped the gene on human chromosome 6p12 by fluorescent in situ hybridization. |
| doi_str_mv | 10.1177/002215540004800704 |
| format | Article |
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Here we demonstrate expression of the mouse and human GCM 1 proteins in placenta. We have prepared a highly specific antibody that recognizes the GCM 1 protein and have used it to assess the temporal and spatial expression profile of the protein. In both mouse and human placenta, the protein is associated with cells that are involved with exchange between maternal and fetal blood supplies: the labyrinthine cells of the mouse placenta and the syncytio- and cytotrophoblasts of the human placenta. 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Here we demonstrate expression of the mouse and human GCM 1 proteins in placenta. We have prepared a highly specific antibody that recognizes the GCM 1 protein and have used it to assess the temporal and spatial expression profile of the protein. In both mouse and human placenta, the protein is associated with cells that are involved with exchange between maternal and fetal blood supplies: the labyrinthine cells of the mouse placenta and the syncytio- and cytotrophoblasts of the human placenta. Using the full-length hGcm 1 cDNA as a probe, we have mapped the gene on human chromosome 6p12 by fluorescent in situ hybridization.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Blotting, Western</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 6</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - immunology</subject><subject>Neuropeptides - metabolism</subject><subject>Nuclear Proteins</subject><subject>Placenta - metabolism</subject><subject>Placenta - ultrastructure</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription Factors</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFLwzAUxoMobk7_AQ_Si97qkjRJk6OMuQ0GetBzyNJk7WibmbRU_ett6Q6C4OnB-37f93gfALcIPiKUpnMIMUaUEggh4RCmkJyBab9AMYWEnIPpAMQDMQFXIRwgRIRQfgkmCHLKMeNTsHktlTZ1o8po-Xn0JoTC1ZGqs2iRe1e54Kpe2jqtyuJbNYPobNTkJlq3laqjla4iFK1Mba7BhVVlMDenOQPvz8u3xTrevqw2i6dtrEkqmhgnECeUMWqhpSTTXFmuiCApzhjdGUQ0wUoxu2OZMVorhkUmkGU8SQW2Aicz8DDmHr37aE1oZFUEbcpS1ca1QaYIcUaF6EE8gtq7ELyx8uiLSvkviaAcCpR_C-xNd6f0dleZ7JdlbKwH5iMQ1N7Ig2t93X_7f-T96MiLfd4V3sjQd1r2B5Dsuo5wmUqBaPID_jyEEA</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Nait-Oumesmar, Brahim</creator><creator>Copperman, Alan B</creator><creator>Lazzarini, Robert A</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Placental Expression and Chromosomal Localization of the Human Gcm 1 Gene</title><author>Nait-Oumesmar, Brahim ; Copperman, Alan B ; Lazzarini, Robert A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-230235665f0f54dc8af8a49472d65be14c42aa6fb6deecca629d91f683792f923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Blotting, Western</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 6</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - immunology</topic><topic>Neuropeptides - metabolism</topic><topic>Nuclear Proteins</topic><topic>Placenta - metabolism</topic><topic>Placenta - ultrastructure</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nait-Oumesmar, Brahim</creatorcontrib><creatorcontrib>Copperman, Alan B</creatorcontrib><creatorcontrib>Lazzarini, Robert A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nait-Oumesmar, Brahim</au><au>Copperman, Alan B</au><au>Lazzarini, Robert A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Placental Expression and Chromosomal Localization of the Human Gcm 1 Gene</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>48</volume><issue>7</issue><spage>915</spage><epage>922</epage><pages>915-922</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Although gcm was first recognized for its role in specifying glial cell fate in Drosophila melanogaster, its mammalian counterparts are expressed predominantly in non-neural tissues. 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| subjects | Amino Acid Sequence Animals Antibody Specificity Blotting, Western Chromosome Mapping Chromosomes, Human, Pair 6 Humans Immune Sera Immunohistochemistry In Situ Hybridization, Fluorescence Lymphocytes - metabolism Mice Molecular Sequence Data Neuropeptides - genetics Neuropeptides - immunology Neuropeptides - metabolism Nuclear Proteins Placenta - metabolism Placenta - ultrastructure Sequence Homology, Amino Acid Transcription Factors |
| title | Placental Expression and Chromosomal Localization of the Human Gcm 1 Gene |
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