Immunofluorescence analysis of cytokine and granule protein expression during eosinophil maturation from cord blood–derived CD34 + progenitors

Background: In allergic inflammation and asthma, eosinophils are major effector cells. They have been shown to synthesize at least 23 cytokines, some of which are stored intracellularly in their unique crystalloid granules together with cationic granule protein. Little is known about the synthesis a...

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Veröffentlicht in:	Journal of allergy and clinical immunology 2000-06, Vol.105 (6), p.1178-1184
Hauptverfasser:	Mahmudi-Azer, Salahaddin, Velazquez, Juan R., Lacy, Paige, Denburg, Judah A., Moqbel, Redwan
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD34 - analysis Biological and medical sciences Blood Proteins - metabolism CD34 + progenitor chemokine Chemokine CCL5 - immunology confocal microscopy cord blood crystalloid granule cytokine Cytokines - metabolism Eosinophil Eosinophil Granule Proteins Eosinophils - cytology Eosinophils - physiology Fetal Blood - immunology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Growth Hematopoiesis Hematopoietic Stem Cells - immunology Humans IL-3 IL-5 IL-6 Immunobiology Interleukin-6 - immunology Myeloid cells: ontogeny, maturation, markers, receptors Polynuclears RANTES Ribonucleases Staining and Labeling
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