Purification and characterization of α- l-fucosidases from Streptomyces sp. OH11242

α- l-Fucosidases were found in the culture fluid of Streptomyces sp. OH11242 grown with porcine gastric mucin (PGM) as the sole carbon source. The α- l-fucosidases were purified by ammonium sulfate precipitation followed by chromatography on Sepharose CL-4B, hydroxyapatite, Resource Q and Mono Q. Tw...

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Veröffentlicht in:	Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2001-10, Vol.130 (3), p.375-383
Hauptverfasser:	Goso, Y, Ishihara, K, Sugawara, S, Hotta, K
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-L-Fucosidase - chemistry alpha-L-Fucosidase - isolation & purification alpha-L-Fucosidase - metabolism Animals Chromatography, Gel Culture Media, Conditioned Electrophoresis, Polyacrylamide Gel Enzyme purification Fucosidase Gastric Mucins - metabolism Glycosidase Glycosides - metabolism Hydrogen-Ion Concentration Hydrolysis Molecular Weight Mucin Oligosaccharide Pyridyl amination Streptomyces - enzymology Streptomyces sp. OH11242 Substrate Specificity Swine
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