Detection and significance of inapparent infection in Chagas disease in western Venezuela
Inapparent infections of Trypanosoma cruzi were detected in symptomless seropositive people living in close proximity, and under the same conditions of risk, to patients with acute Chagas disease. Similar infections were also detected in sera samples of people from 25 villages of western Venezuela w...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2001-09, Vol.65 (3), p.227-232 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Anez, N Crisante, G Rojas, A Carrasco, H Parada, H Yepez, Y Borges, R Guevara, P Ramirez, JL |
| description | Inapparent infections of Trypanosoma cruzi were detected in symptomless seropositive people living in close proximity, and under the same conditions of risk, to patients with acute Chagas disease. Similar infections were also detected in sera samples of people from 25 villages of western Venezuela where Chagas disease is endemic. Seropositivity in all the 1,251 studied samples was established by use of 3 serological methods (direct agglutination test, indirect immunofluorescence antibody test, and enzyme-linked immunosorbent assay). Each seropositive sample was tested for detection of anti-T. cruzi-specific immunoglobulin (Ig) M and IgG levels and specific T. cruzi infection by molecular methodology (polymerase chain reaction assay). The combined analysis of the serologic (IgM and IgG levels), molecular (specific T. cruzi DNA), and statistical findings demonstrated the existence of a different stage of T. cruzi infection in asymptomatic patients, which is suggested to be recognized as inapparent infection. Its definition, significance, and comparison with typical Chagas disease phases are presented, and its potential epidemiological importance is discussed. |
| doi_str_mv | 10.4269/ajtmh.2001.65.227 |
| format | Article |
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Similar infections were also detected in sera samples of people from 25 villages of western Venezuela where Chagas disease is endemic. Seropositivity in all the 1,251 studied samples was established by use of 3 serological methods (direct agglutination test, indirect immunofluorescence antibody test, and enzyme-linked immunosorbent assay). Each seropositive sample was tested for detection of anti-T. cruzi-specific immunoglobulin (Ig) M and IgG levels and specific T. cruzi infection by molecular methodology (polymerase chain reaction assay). The combined analysis of the serologic (IgM and IgG levels), molecular (specific T. cruzi DNA), and statistical findings demonstrated the existence of a different stage of T. cruzi infection in asymptomatic patients, which is suggested to be recognized as inapparent infection. Its definition, significance, and comparison with typical Chagas disease phases are presented, and its potential epidemiological importance is discussed.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.2001.65.227</identifier><identifier>PMID: 11561709</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Lawrence, KS: ASTMH</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antibodies, Protozoan - blood ; Biological and medical sciences ; Chagas Disease - blood ; Chagas Disease - diagnosis ; Chagas Disease - epidemiology ; Chagas Disease - parasitology ; Child ; Child, Preschool ; DNA, Protozoan - analysis ; Endemic Diseases ; Enzyme-Linked Immunosorbent Assay ; Female ; Human protozoal diseases ; Humans ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Infant ; Infectious diseases ; Male ; Medical sciences ; Middle Aged ; Parasitic diseases ; Polymerase Chain Reaction ; Protozoal diseases ; Seroepidemiologic Studies ; Tropical medicine ; Trypanosoma cruzi ; Trypanosoma cruzi - genetics ; Trypanosoma cruzi - immunology ; Trypanosoma cruzi - isolation & purification ; Trypanosomiasis ; Venezuela - epidemiology</subject><ispartof>The American journal of tropical medicine and hygiene, 2001-09, Vol.65 (3), p.227-232</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-5ac590bb1ca431268cd4bf0875d9f373d10716bf5dc812ef307fcddd74cddc823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14057549$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11561709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anez, N</creatorcontrib><creatorcontrib>Crisante, G</creatorcontrib><creatorcontrib>Rojas, A</creatorcontrib><creatorcontrib>Carrasco, H</creatorcontrib><creatorcontrib>Parada, H</creatorcontrib><creatorcontrib>Yepez, Y</creatorcontrib><creatorcontrib>Borges, R</creatorcontrib><creatorcontrib>Guevara, P</creatorcontrib><creatorcontrib>Ramirez, JL</creatorcontrib><title>Detection and significance of inapparent infection in Chagas disease in western Venezuela</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Inapparent infections of Trypanosoma cruzi were detected in symptomless seropositive people living in close proximity, and under the same conditions of risk, to patients with acute Chagas disease. Similar infections were also detected in sera samples of people from 25 villages of western Venezuela where Chagas disease is endemic. Seropositivity in all the 1,251 studied samples was established by use of 3 serological methods (direct agglutination test, indirect immunofluorescence antibody test, and enzyme-linked immunosorbent assay). Each seropositive sample was tested for detection of anti-T. cruzi-specific immunoglobulin (Ig) M and IgG levels and specific T. cruzi infection by molecular methodology (polymerase chain reaction assay). The combined analysis of the serologic (IgM and IgG levels), molecular (specific T. cruzi DNA), and statistical findings demonstrated the existence of a different stage of T. cruzi infection in asymptomatic patients, which is suggested to be recognized as inapparent infection. Its definition, significance, and comparison with typical Chagas disease phases are presented, and its potential epidemiological importance is discussed.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Biological and medical sciences</subject><subject>Chagas Disease - blood</subject><subject>Chagas Disease - diagnosis</subject><subject>Chagas Disease - epidemiology</subject><subject>Chagas Disease - parasitology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>DNA, Protozoan - analysis</subject><subject>Endemic Diseases</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Parasitic diseases</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoal diseases</subject><subject>Seroepidemiologic Studies</subject><subject>Tropical medicine</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - genetics</subject><subject>Trypanosoma cruzi - immunology</subject><subject>Trypanosoma cruzi - isolation & purification</subject><subject>Trypanosomiasis</subject><subject>Venezuela - epidemiology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhB3BBucAti8fxR3JEy6dUqZcWiZPl2ONdV4mz2FlF8OvrbSOVGxePPXrmHesh5C3QLWey-2ju5vGwZZTCVootY-oZ2QBXsgbJxXOyoZSyupONuiCvcr4rXMsAXpILACFB0W5Dfn3GGe0cpliZ6Koc9jH4YE20WE2-CtEcjyZhnMvVr2CI1e5g9iZXLmQ0Gc-dBfOMKVY_MeLfEw7mNXnhzZDxzVovye3XLze77_XV9bcfu09XteUNzLUwVnS078Ga8maytY73nrZKuM43qnFAFcjeC2dbYOgbqrx1zileTtuy5pJ8eMw9pun3qfxCjyFbHAYTcTplrQBaaKT8L1jkCNZyXkB4BG2ack7o9TGF0aQ_Gqg-i9cP4vVZvJZCF_Fl5t0afupHdE8Tq-kCvF8Bk60ZfCqKQ37iOBVK8H-4Q9gflpBQ59EMQ4kFvSxLWdc8LLwHE2qaoA</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Anez, N</creator><creator>Crisante, G</creator><creator>Rojas, A</creator><creator>Carrasco, H</creator><creator>Parada, H</creator><creator>Yepez, Y</creator><creator>Borges, R</creator><creator>Guevara, P</creator><creator>Ramirez, JL</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>Detection and significance of inapparent infection in Chagas disease in western Venezuela</title><author>Anez, N ; Crisante, G ; Rojas, A ; Carrasco, H ; Parada, H ; Yepez, Y ; Borges, R ; Guevara, P ; Ramirez, JL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-5ac590bb1ca431268cd4bf0875d9f373d10716bf5dc812ef307fcddd74cddc823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Biological and medical sciences</topic><topic>Chagas Disease - blood</topic><topic>Chagas Disease - diagnosis</topic><topic>Chagas Disease - epidemiology</topic><topic>Chagas Disease - parasitology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>DNA, Protozoan - analysis</topic><topic>Endemic Diseases</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Parasitic diseases</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoal diseases</topic><topic>Seroepidemiologic Studies</topic><topic>Tropical medicine</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - genetics</topic><topic>Trypanosoma cruzi - immunology</topic><topic>Trypanosoma cruzi - isolation & purification</topic><topic>Trypanosomiasis</topic><topic>Venezuela - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anez, N</creatorcontrib><creatorcontrib>Crisante, G</creatorcontrib><creatorcontrib>Rojas, A</creatorcontrib><creatorcontrib>Carrasco, H</creatorcontrib><creatorcontrib>Parada, H</creatorcontrib><creatorcontrib>Yepez, Y</creatorcontrib><creatorcontrib>Borges, R</creatorcontrib><creatorcontrib>Guevara, P</creatorcontrib><creatorcontrib>Ramirez, JL</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anez, N</au><au>Crisante, G</au><au>Rojas, A</au><au>Carrasco, H</au><au>Parada, H</au><au>Yepez, Y</au><au>Borges, R</au><au>Guevara, P</au><au>Ramirez, JL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and significance of inapparent infection in Chagas disease in western Venezuela</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>65</volume><issue>3</issue><spage>227</spage><epage>232</epage><pages>227-232</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>Inapparent infections of Trypanosoma cruzi were detected in symptomless seropositive people living in close proximity, and under the same conditions of risk, to patients with acute Chagas disease. Similar infections were also detected in sera samples of people from 25 villages of western Venezuela where Chagas disease is endemic. Seropositivity in all the 1,251 studied samples was established by use of 3 serological methods (direct agglutination test, indirect immunofluorescence antibody test, and enzyme-linked immunosorbent assay). Each seropositive sample was tested for detection of anti-T. cruzi-specific immunoglobulin (Ig) M and IgG levels and specific T. cruzi infection by molecular methodology (polymerase chain reaction assay). The combined analysis of the serologic (IgM and IgG levels), molecular (specific T. cruzi DNA), and statistical findings demonstrated the existence of a different stage of T. cruzi infection in asymptomatic patients, which is suggested to be recognized as inapparent infection. Its definition, significance, and comparison with typical Chagas disease phases are presented, and its potential epidemiological importance is discussed.</abstract><cop>Lawrence, KS</cop><pub>ASTMH</pub><pmid>11561709</pmid><doi>10.4269/ajtmh.2001.65.227</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Animals Antibodies, Protozoan - blood Biological and medical sciences Chagas Disease - blood Chagas Disease - diagnosis Chagas Disease - epidemiology Chagas Disease - parasitology Child Child, Preschool DNA, Protozoan - analysis Endemic Diseases Enzyme-Linked Immunosorbent Assay Female Human protozoal diseases Humans Immunoglobulin G - blood Immunoglobulin M - blood Infant Infectious diseases Male Medical sciences Middle Aged Parasitic diseases Polymerase Chain Reaction Protozoal diseases Seroepidemiologic Studies Tropical medicine Trypanosoma cruzi Trypanosoma cruzi - genetics Trypanosoma cruzi - immunology Trypanosoma cruzi - isolation & purification Trypanosomiasis Venezuela - epidemiology |
| title | Detection and significance of inapparent infection in Chagas disease in western Venezuela |
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