Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system
Pediocin PA-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. However, such use may lead to the development of resistance in the target organism. Gene expression in two independent pediocin-resistant mutants of Listeria monocytogenes 412 was comp...
Gespeichert in:
| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2000-06, Vol.146 (6), p.1381-1389 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1389 |
|---|---|
| container_issue | 6 |
| container_start_page | 1381 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 146 |
| creator | GRAVESEN, A WARTHOE, P KNØCHEL, S THIRSTRUP, K |
| description | Pediocin PA-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. However, such use may lead to the development of resistance in the target organism. Gene expression in two independent pediocin-resistant mutants of Listeria monocytogenes 412 was compared to the original isolate by restriction fragment differential display PCR (RFDD-PCR). This method amplifies cDNA restriction fragments under stringent PCR conditions, enabled by the use of specific primers complementary to ligated adaptor sequences. RFDD-PCR was very well suited for analysis of listerial gene expression, giving reproducible PCR product profiles. Three gene fragments having increased expression in both resistant mutants were identified. All three had homology to components of beta-glucoside-specific phosphoenolpyruvate-dependent phosphotransferase systems (PTS), one fragment having homology to enzyme II permeases, and the two others to phospho-beta-glucosidases. Overexpression of the putative PTS system was consistently observed in 10 additional pediocin-resistant mutants, isolated at different pH, salt content and temperature. The results suggest that RFDD-PCR is a strong approach for the analysis of prokaryotic gene expression and that the putative beta-glucoside-specific PTS system is involved in mediating pediocin resistance. |
| doi_str_mv | 10.1099/00221287-146-6-1381 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71176427</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17754785</sourcerecordid><originalsourceid>FETCH-LOGICAL-p267t-c3cb67a664f42a2fafaf9af57b008b6e5e60f52fbfd18300c5d2d3fb89915cf83</originalsourceid><addsrcrecordid>eNqFkc1u1DAQgC1ERX_gCZCQD6g3w9iOf3KsqpYirdSqLeeV49iLURKHTFK6j0UfhGfCKxb1WM1hZjTffJY8hLzn8IlDXX8GEIILaxivNNOMS8tfkaPSKCbAwutSSwUMrBGH5BjxB0AZAn9DDjnYSguuj8jv24DzlPyc8kDj5DZ9GGbaphjDVKrkutLg2LktzZGOoU3Zp4FNARPOrqCrksOUHO3zkP12zpswBKQVF7RfdgRS_J5_IfV52O3s9PmhyB_HIsHds0Xs6FjgOT0E-ueJbbrFZ0xtYDgGn2Ly9Ob-juK2rPdvyUF0HYZ3-3xCvl1e3J9fsdX1l6_nZys2Cm1m5qVvtHFaV7ESTkRXonZRmQbANjqooCEqEZvYcisBvGpFK2Nj65orH608Iaf_vOOUfy7ll9Z9Qh-6zg0hL7g2nBtdCfMiyI1RlbGqgB_24NL0oV2PU-rdtF3_v0YBPu4Bh9515RyDT_jMVVIqAfIv2t-gJg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17754785</pqid></control><display><type>article</type><title>Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system</title><source>MEDLINE</source><source>PubMed Central</source><creator>GRAVESEN, A ; WARTHOE, P ; KNØCHEL, S ; THIRSTRUP, K</creator><creatorcontrib>GRAVESEN, A ; WARTHOE, P ; KNØCHEL, S ; THIRSTRUP, K</creatorcontrib><description>Pediocin PA-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. However, such use may lead to the development of resistance in the target organism. Gene expression in two independent pediocin-resistant mutants of Listeria monocytogenes 412 was compared to the original isolate by restriction fragment differential display PCR (RFDD-PCR). This method amplifies cDNA restriction fragments under stringent PCR conditions, enabled by the use of specific primers complementary to ligated adaptor sequences. RFDD-PCR was very well suited for analysis of listerial gene expression, giving reproducible PCR product profiles. Three gene fragments having increased expression in both resistant mutants were identified. All three had homology to components of beta-glucoside-specific phosphoenolpyruvate-dependent phosphotransferase systems (PTS), one fragment having homology to enzyme II permeases, and the two others to phospho-beta-glucosidases. Overexpression of the putative PTS system was consistently observed in 10 additional pediocin-resistant mutants, isolated at different pH, salt content and temperature. The results suggest that RFDD-PCR is a strong approach for the analysis of prokaryotic gene expression and that the putative beta-glucoside-specific PTS system is involved in mediating pediocin resistance.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/00221287-146-6-1381</identifier><identifier>PMID: 10846216</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Amino Acid Sequence ; Bacteriocins - pharmacology ; Bacteriology ; Base Sequence ; beta -Glucoside ; Biological and medical sciences ; Biotechnology ; DNA Primers - genetics ; Drug Resistance, Microbial - genetics ; Food Preservation ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genetics ; Humans ; Listeria monocytogenes ; Listeria monocytogenes - drug effects ; Listeria monocytogenes - genetics ; Listeria monocytogenes - pathogenicity ; Methods. Procedures. Technologies ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Mutation ; pediocin PA-1 ; Pediocins ; Phosphoenolpyruvate Sugar Phosphotransferase System - genetics ; Polymerase Chain Reaction - methods ; PTS system ; Sequence Homology, Amino Acid ; Synthetic digonucleotides and genes. Sequencing</subject><ispartof>Microbiology (Society for General Microbiology), 2000-06, Vol.146 (6), p.1381-1389</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1433520$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10846216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRAVESEN, A</creatorcontrib><creatorcontrib>WARTHOE, P</creatorcontrib><creatorcontrib>KNØCHEL, S</creatorcontrib><creatorcontrib>THIRSTRUP, K</creatorcontrib><title>Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Pediocin PA-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. However, such use may lead to the development of resistance in the target organism. Gene expression in two independent pediocin-resistant mutants of Listeria monocytogenes 412 was compared to the original isolate by restriction fragment differential display PCR (RFDD-PCR). This method amplifies cDNA restriction fragments under stringent PCR conditions, enabled by the use of specific primers complementary to ligated adaptor sequences. RFDD-PCR was very well suited for analysis of listerial gene expression, giving reproducible PCR product profiles. Three gene fragments having increased expression in both resistant mutants were identified. All three had homology to components of beta-glucoside-specific phosphoenolpyruvate-dependent phosphotransferase systems (PTS), one fragment having homology to enzyme II permeases, and the two others to phospho-beta-glucosidases. Overexpression of the putative PTS system was consistently observed in 10 additional pediocin-resistant mutants, isolated at different pH, salt content and temperature. The results suggest that RFDD-PCR is a strong approach for the analysis of prokaryotic gene expression and that the putative beta-glucoside-specific PTS system is involved in mediating pediocin resistance.</description><subject>Amino Acid Sequence</subject><subject>Bacteriocins - pharmacology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>beta -Glucoside</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>DNA Primers - genetics</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Food Preservation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetics</subject><subject>Humans</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - drug effects</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>pediocin PA-1</subject><subject>Pediocins</subject><subject>Phosphoenolpyruvate Sugar Phosphotransferase System - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>PTS system</subject><subject>Sequence Homology, Amino Acid</subject><subject>Synthetic digonucleotides and genes. Sequencing</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAQgC1ERX_gCZCQD6g3w9iOf3KsqpYirdSqLeeV49iLURKHTFK6j0UfhGfCKxb1WM1hZjTffJY8hLzn8IlDXX8GEIILaxivNNOMS8tfkaPSKCbAwutSSwUMrBGH5BjxB0AZAn9DDjnYSguuj8jv24DzlPyc8kDj5DZ9GGbaphjDVKrkutLg2LktzZGOoU3Zp4FNARPOrqCrksOUHO3zkP12zpswBKQVF7RfdgRS_J5_IfV52O3s9PmhyB_HIsHds0Xs6FjgOT0E-ueJbbrFZ0xtYDgGn2Ly9Ob-juK2rPdvyUF0HYZ3-3xCvl1e3J9fsdX1l6_nZys2Cm1m5qVvtHFaV7ESTkRXonZRmQbANjqooCEqEZvYcisBvGpFK2Nj65orH608Iaf_vOOUfy7ll9Z9Qh-6zg0hL7g2nBtdCfMiyI1RlbGqgB_24NL0oV2PU-rdtF3_v0YBPu4Bh9515RyDT_jMVVIqAfIv2t-gJg</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>GRAVESEN, A</creator><creator>WARTHOE, P</creator><creator>KNØCHEL, S</creator><creator>THIRSTRUP, K</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system</title><author>GRAVESEN, A ; WARTHOE, P ; KNØCHEL, S ; THIRSTRUP, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p267t-c3cb67a664f42a2fafaf9af57b008b6e5e60f52fbfd18300c5d2d3fb89915cf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteriocins - pharmacology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>beta -Glucoside</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>DNA Primers - genetics</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Food Preservation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetics</topic><topic>Humans</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - drug effects</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>pediocin PA-1</topic><topic>Pediocins</topic><topic>Phosphoenolpyruvate Sugar Phosphotransferase System - genetics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>PTS system</topic><topic>Sequence Homology, Amino Acid</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRAVESEN, A</creatorcontrib><creatorcontrib>WARTHOE, P</creatorcontrib><creatorcontrib>KNØCHEL, S</creatorcontrib><creatorcontrib>THIRSTRUP, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GRAVESEN, A</au><au>WARTHOE, P</au><au>KNØCHEL, S</au><au>THIRSTRUP, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>146</volume><issue>6</issue><spage>1381</spage><epage>1389</epage><pages>1381-1389</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Pediocin PA-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. However, such use may lead to the development of resistance in the target organism. Gene expression in two independent pediocin-resistant mutants of Listeria monocytogenes 412 was compared to the original isolate by restriction fragment differential display PCR (RFDD-PCR). This method amplifies cDNA restriction fragments under stringent PCR conditions, enabled by the use of specific primers complementary to ligated adaptor sequences. RFDD-PCR was very well suited for analysis of listerial gene expression, giving reproducible PCR product profiles. Three gene fragments having increased expression in both resistant mutants were identified. All three had homology to components of beta-glucoside-specific phosphoenolpyruvate-dependent phosphotransferase systems (PTS), one fragment having homology to enzyme II permeases, and the two others to phospho-beta-glucosidases. Overexpression of the putative PTS system was consistently observed in 10 additional pediocin-resistant mutants, isolated at different pH, salt content and temperature. The results suggest that RFDD-PCR is a strong approach for the analysis of prokaryotic gene expression and that the putative beta-glucoside-specific PTS system is involved in mediating pediocin resistance.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>10846216</pmid><doi>10.1099/00221287-146-6-1381</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1350-0872 |
| ispartof | Microbiology (Society for General Microbiology), 2000-06, Vol.146 (6), p.1381-1389 |
| issn | 1350-0872 1465-2080 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71176427 |
| source | MEDLINE; PubMed Central |
| subjects | Amino Acid Sequence Bacteriocins - pharmacology Bacteriology Base Sequence beta -Glucoside Biological and medical sciences Biotechnology DNA Primers - genetics Drug Resistance, Microbial - genetics Food Preservation Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Genetic engineering Genetic technics Genetics Humans Listeria monocytogenes Listeria monocytogenes - drug effects Listeria monocytogenes - genetics Listeria monocytogenes - pathogenicity Methods. Procedures. Technologies Microbiology Miscellaneous Molecular Sequence Data Mutation pediocin PA-1 Pediocins Phosphoenolpyruvate Sugar Phosphotransferase System - genetics Polymerase Chain Reaction - methods PTS system Sequence Homology, Amino Acid Synthetic digonucleotides and genes. Sequencing |
| title | Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative β-glucoside-specific PTS system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A37%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Restriction%20fragment%20differential%20display%20of%20pediocin-resistant%20Listeria%20monocytogenes%20412%20mutants%20shows%20consistent%20overexpression%20of%20a%20putative%20%CE%B2-glucoside-specific%20PTS%20system&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=GRAVESEN,%20A&rft.date=2000-06-01&rft.volume=146&rft.issue=6&rft.spage=1381&rft.epage=1389&rft.pages=1381-1389&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/00221287-146-6-1381&rft_dat=%3Cproquest_pubme%3E17754785%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17754785&rft_id=info:pmid/10846216&rfr_iscdi=true |