Protective effects of Clostridium sordellii LT and HT toxoids against challenge with spores in Guinea pigs
The protective effects of Clostridium sordellii lethal toxin (LT) and hemorrhagic toxin (HT) toxoids against challenge with spores in guinea pigs were investigated. Purified LT and partially purified HT were obtained from the culture supernatant of C. sordellii strain 3703, and then were treated wit...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2001, Vol.63(8), pp.879-883 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Amimoto, K. (Kyoto Biken Lab., Uji (Japan)) Oishi, E Yasuhara, H Sasaki, O Katayama, S Kitajima, T Izumida, A Hirahara, T |
| description | The protective effects of Clostridium sordellii lethal toxin (LT) and hemorrhagic toxin (HT) toxoids against challenge with spores in guinea pigs were investigated. Purified LT and partially purified HT were obtained from the culture supernatant of C. sordellii strain 3703, and then were treated with formalin to make toxoids. LT, HT and combined LT and HT (LT / HT) toxoid vaccines were prepared by mixing each toxoid with an aluminum phosphate gel as adjuvant. Guinea pigs immunized twice with the respective toxoid vaccines were challenged with spores of strains 3703 or KZ 1047. The latter strain does not produce HT. LT toxoid vaccine conferred protection against challenge with strain KZ1047, but not strain 3703, in guinea pigs. All guinea pigs immunized with HT toxoid vaccine died after challenge with spores of either strain. LT / HT toxoid vaccine gave complete protection against challenge with spores of strains 3703 and KZ1047 to guinea pigs. These results suggest that not only LT toxoid, but also HT toxoid, are essential protective antigens of C. sordellii. |
| doi_str_mv | 10.1292/jvms.63.879 |
| format | Article |
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(Kyoto Biken Lab., Uji (Japan)) ; Oishi, E ; Yasuhara, H ; Sasaki, O ; Katayama, S ; Kitajima, T ; Izumida, A ; Hirahara, T</creator><creatorcontrib>Amimoto, K. (Kyoto Biken Lab., Uji (Japan)) ; Oishi, E ; Yasuhara, H ; Sasaki, O ; Katayama, S ; Kitajima, T ; Izumida, A ; Hirahara, T</creatorcontrib><description>The protective effects of Clostridium sordellii lethal toxin (LT) and hemorrhagic toxin (HT) toxoids against challenge with spores in guinea pigs were investigated. Purified LT and partially purified HT were obtained from the culture supernatant of C. sordellii strain 3703, and then were treated with formalin to make toxoids. LT, HT and combined LT and HT (LT / HT) toxoid vaccines were prepared by mixing each toxoid with an aluminum phosphate gel as adjuvant. Guinea pigs immunized twice with the respective toxoid vaccines were challenged with spores of strains 3703 or KZ 1047. The latter strain does not produce HT. LT toxoid vaccine conferred protection against challenge with strain KZ1047, but not strain 3703, in guinea pigs. All guinea pigs immunized with HT toxoid vaccine died after challenge with spores of either strain. LT / HT toxoid vaccine gave complete protection against challenge with spores of strains 3703 and KZ1047 to guinea pigs. These results suggest that not only LT toxoid, but also HT toxoid, are essential protective antigens of C. sordellii.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.63.879</identifier><identifier>PMID: 11558543</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adjuvants, Immunologic ; Aluminum Compounds - immunology ; Animals ; ANTIGENS ; Bacterial Toxins - immunology ; Bacterial Vaccines - immunology ; Blotting, Western - veterinary ; Cercopithecus aethiops ; CLOSTRIDIUM ; Clostridium - growth & development ; Clostridium - immunology ; Clostridium Infections - immunology ; Clostridium Infections - microbiology ; Clostridium Infections - prevention & control ; Clostridium sordellii ; Electrophoresis, Polyacrylamide Gel ; Female ; GUINEA PIGS ; HAEMORRHAGE ; Immunization - veterinary ; Male ; Neutralization Tests - veterinary ; Phosphates - immunology ; SPORES ; TOXINS ; toxoid ; Toxoids - immunology ; Vero Cells</subject><ispartof>Journal of Veterinary Medical Science, 2001, Vol.63(8), pp.879-883</ispartof><rights>2001 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-617f90518ce6ba68b08f679ff0bf6ead2c4376bd44c980fc84b59d2001c53dbb3</citedby><cites>FETCH-LOGICAL-c550t-617f90518ce6ba68b08f679ff0bf6ead2c4376bd44c980fc84b59d2001c53dbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11558543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amimoto, K. (Kyoto Biken Lab., Uji (Japan))</creatorcontrib><creatorcontrib>Oishi, E</creatorcontrib><creatorcontrib>Yasuhara, H</creatorcontrib><creatorcontrib>Sasaki, O</creatorcontrib><creatorcontrib>Katayama, S</creatorcontrib><creatorcontrib>Kitajima, T</creatorcontrib><creatorcontrib>Izumida, A</creatorcontrib><creatorcontrib>Hirahara, T</creatorcontrib><title>Protective effects of Clostridium sordellii LT and HT toxoids against challenge with spores in Guinea pigs</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The protective effects of Clostridium sordellii lethal toxin (LT) and hemorrhagic toxin (HT) toxoids against challenge with spores in guinea pigs were investigated. Purified LT and partially purified HT were obtained from the culture supernatant of C. sordellii strain 3703, and then were treated with formalin to make toxoids. LT, HT and combined LT and HT (LT / HT) toxoid vaccines were prepared by mixing each toxoid with an aluminum phosphate gel as adjuvant. Guinea pigs immunized twice with the respective toxoid vaccines were challenged with spores of strains 3703 or KZ 1047. The latter strain does not produce HT. LT toxoid vaccine conferred protection against challenge with strain KZ1047, but not strain 3703, in guinea pigs. All guinea pigs immunized with HT toxoid vaccine died after challenge with spores of either strain. LT / HT toxoid vaccine gave complete protection against challenge with spores of strains 3703 and KZ1047 to guinea pigs. These results suggest that not only LT toxoid, but also HT toxoid, are essential protective antigens of C. sordellii.</description><subject>Adjuvants, Immunologic</subject><subject>Aluminum Compounds - immunology</subject><subject>Animals</subject><subject>ANTIGENS</subject><subject>Bacterial Toxins - immunology</subject><subject>Bacterial Vaccines - immunology</subject><subject>Blotting, Western - veterinary</subject><subject>Cercopithecus aethiops</subject><subject>CLOSTRIDIUM</subject><subject>Clostridium - growth & development</subject><subject>Clostridium - immunology</subject><subject>Clostridium Infections - immunology</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - prevention & control</subject><subject>Clostridium sordellii</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>GUINEA PIGS</subject><subject>HAEMORRHAGE</subject><subject>Immunization - veterinary</subject><subject>Male</subject><subject>Neutralization Tests - veterinary</subject><subject>Phosphates - immunology</subject><subject>SPORES</subject><subject>TOXINS</subject><subject>toxoid</subject><subject>Toxoids - immunology</subject><subject>Vero Cells</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-L1DAYhoMo7rh68qwEBC_SMWmaX7eVQXeVAfcwnkOaJt2UthmTdNX_3gwddsGLly8f5OGB730BeI3RFtey_jjcT2nLyFZw-QRsMGl4xRsin4INkphVvKboArxIaUCoxg2Tz8EFxpQK2pANGG5jyNZkf2-hda5sCQYHd2NIOfrOLxNMIXZ2HL2H-wPUcwdvDjCH38F3Cepe-zllaO70ONq5t_CXz3cwHUO0CfoZXi9-thoefZ9egmdOj8m-Or-X4MeXz4fdTbX_fv1192lfGUpRrhjmTiKKhbGs1Uy0SDjGpXOodczqrjYN4aztmsZIgZwRTUtlVyOEDSVd25JL8H71HmP4udiU1eSTKRfo2YYlKY4xr4VE_wWxpDVjDS_gu3_AISxxLkeoEqhggghy0n1YKRNDStE6dYx-0vGPwkidmlKnphQjqjRV6Ldn59JOtntkz9UU4GoFhpR1bx8AHbM3o32UraM4H75KG1HZuSjerAqng9J99El9uy1JncLCUpC_qcivxg</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Amimoto, K. (Kyoto Biken Lab., Uji (Japan))</creator><creator>Oishi, E</creator><creator>Yasuhara, H</creator><creator>Sasaki, O</creator><creator>Katayama, S</creator><creator>Kitajima, T</creator><creator>Izumida, A</creator><creator>Hirahara, T</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Protective effects of Clostridium sordellii LT and HT toxoids against challenge with spores in Guinea pigs</title><author>Amimoto, K. (Kyoto Biken Lab., Uji (Japan)) ; Oishi, E ; Yasuhara, H ; Sasaki, O ; Katayama, S ; Kitajima, T ; Izumida, A ; Hirahara, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-617f90518ce6ba68b08f679ff0bf6ead2c4376bd44c980fc84b59d2001c53dbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adjuvants, Immunologic</topic><topic>Aluminum Compounds - immunology</topic><topic>Animals</topic><topic>ANTIGENS</topic><topic>Bacterial Toxins - immunology</topic><topic>Bacterial Vaccines - immunology</topic><topic>Blotting, Western - veterinary</topic><topic>Cercopithecus aethiops</topic><topic>CLOSTRIDIUM</topic><topic>Clostridium - growth & development</topic><topic>Clostridium - immunology</topic><topic>Clostridium Infections - immunology</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - prevention & control</topic><topic>Clostridium sordellii</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>GUINEA PIGS</topic><topic>HAEMORRHAGE</topic><topic>Immunization - veterinary</topic><topic>Male</topic><topic>Neutralization Tests - veterinary</topic><topic>Phosphates - immunology</topic><topic>SPORES</topic><topic>TOXINS</topic><topic>toxoid</topic><topic>Toxoids - immunology</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amimoto, K. (Kyoto Biken Lab., Uji (Japan))</creatorcontrib><creatorcontrib>Oishi, E</creatorcontrib><creatorcontrib>Yasuhara, H</creatorcontrib><creatorcontrib>Sasaki, O</creatorcontrib><creatorcontrib>Katayama, S</creatorcontrib><creatorcontrib>Kitajima, T</creatorcontrib><creatorcontrib>Izumida, A</creatorcontrib><creatorcontrib>Hirahara, T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amimoto, K. (Kyoto Biken Lab., Uji (Japan))</au><au>Oishi, E</au><au>Yasuhara, H</au><au>Sasaki, O</au><au>Katayama, S</au><au>Kitajima, T</au><au>Izumida, A</au><au>Hirahara, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects of Clostridium sordellii LT and HT toxoids against challenge with spores in Guinea pigs</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>63</volume><issue>8</issue><spage>879</spage><epage>883</epage><pages>879-883</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The protective effects of Clostridium sordellii lethal toxin (LT) and hemorrhagic toxin (HT) toxoids against challenge with spores in guinea pigs were investigated. Purified LT and partially purified HT were obtained from the culture supernatant of C. sordellii strain 3703, and then were treated with formalin to make toxoids. LT, HT and combined LT and HT (LT / HT) toxoid vaccines were prepared by mixing each toxoid with an aluminum phosphate gel as adjuvant. Guinea pigs immunized twice with the respective toxoid vaccines were challenged with spores of strains 3703 or KZ 1047. The latter strain does not produce HT. LT toxoid vaccine conferred protection against challenge with strain KZ1047, but not strain 3703, in guinea pigs. All guinea pigs immunized with HT toxoid vaccine died after challenge with spores of either strain. LT / HT toxoid vaccine gave complete protection against challenge with spores of strains 3703 and KZ1047 to guinea pigs. These results suggest that not only LT toxoid, but also HT toxoid, are essential protective antigens of C. sordellii.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>11558543</pmid><doi>10.1292/jvms.63.879</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adjuvants, Immunologic Aluminum Compounds - immunology Animals ANTIGENS Bacterial Toxins - immunology Bacterial Vaccines - immunology Blotting, Western - veterinary Cercopithecus aethiops CLOSTRIDIUM Clostridium - growth & development Clostridium - immunology Clostridium Infections - immunology Clostridium Infections - microbiology Clostridium Infections - prevention & control Clostridium sordellii Electrophoresis, Polyacrylamide Gel Female GUINEA PIGS HAEMORRHAGE Immunization - veterinary Male Neutralization Tests - veterinary Phosphates - immunology SPORES TOXINS toxoid Toxoids - immunology Vero Cells |
| title | Protective effects of Clostridium sordellii LT and HT toxoids against challenge with spores in Guinea pigs |
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