Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit
The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody co...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2001-09, Vol.505 (1), p.27-30 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 30 |
|---|---|
| container_issue | 1 |
| container_start_page | 27 |
| container_title | FEBS letters |
| container_volume | 505 |
| creator | Tan, S M Walters, S E Mathew, E C Robinson, M K Drbal, K Shaw, J M Law, S K |
| description | The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues. |
| doi_str_mv | 10.1016/S0014-5793(01)02778-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71170660</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71170660</sourcerecordid><originalsourceid>FETCH-LOGICAL-p139t-ddfcd2f147d5daa2ff23d6a72ef84485968b21d0bbeaab9d0bf95892722738753</originalsourceid><addsrcrecordid>eNo9kEtPwzAQhH0A0VL4CaCcUHsI-JHE9hGlvKRKSAXOkROvU6PECbFz6L8nlMJpdma_3cMgdEXwLcEku3vDmCRxyiVbYrLClHMRixM0_49n6Nz7Tzx5QeQZmhGSphyzbI5gDcY66-oo7CAaoAcVfhw00IILPrLusKn2PoB1EA-22k1cbTsXLfPtdhV15kDkayImOkA9TDclBBXRyI_l6Gy4QKdGNR4uj7pAH48P7_lzvHl9esnvN3FPmAyx1qbS1JCE61QrRY2hTGeKUzAiSUQqM1FSonFZglKlnAYjUyEpp5QzwVO2QDe_f_uh-xrBh6K1voKmUQ660RecEI6zDE_g9REcyxZ00Q-2VcO--CuGfQP62mO1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71170660</pqid></control><display><type>article</type><title>Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elsevier ScienceDirect Journals</source><source>Wiley Online Library Free Content</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Tan, S M ; Walters, S E ; Mathew, E C ; Robinson, M K ; Drbal, K ; Shaw, J M ; Law, S K</creator><creatorcontrib>Tan, S M ; Walters, S E ; Mathew, E C ; Robinson, M K ; Drbal, K ; Shaw, J M ; Law, S K</creatorcontrib><description>The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues.</description><identifier>ISSN: 0014-5793</identifier><identifier>DOI: 10.1016/S0014-5793(01)02778-8</identifier><identifier>PMID: 11557036</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; CD18 Antigens - genetics ; CD18 Antigens - immunology ; CD4 Antigens - genetics ; CD4 Antigens - metabolism ; COS Cells ; Cysteine ; Epitopes - chemistry ; Molecular Sequence Data ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Repetitive Sequences, Amino Acid</subject><ispartof>FEBS letters, 2001-09, Vol.505 (1), p.27-30</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11557036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, S M</creatorcontrib><creatorcontrib>Walters, S E</creatorcontrib><creatorcontrib>Mathew, E C</creatorcontrib><creatorcontrib>Robinson, M K</creatorcontrib><creatorcontrib>Drbal, K</creatorcontrib><creatorcontrib>Shaw, J M</creatorcontrib><creatorcontrib>Law, S K</creatorcontrib><title>Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>CD18 Antigens - genetics</subject><subject>CD18 Antigens - immunology</subject><subject>CD4 Antigens - genetics</subject><subject>CD4 Antigens - metabolism</subject><subject>COS Cells</subject><subject>Cysteine</subject><subject>Epitopes - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Repetitive Sequences, Amino Acid</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhH0A0VL4CaCcUHsI-JHE9hGlvKRKSAXOkROvU6PECbFz6L8nlMJpdma_3cMgdEXwLcEku3vDmCRxyiVbYrLClHMRixM0_49n6Nz7Tzx5QeQZmhGSphyzbI5gDcY66-oo7CAaoAcVfhw00IILPrLusKn2PoB1EA-22k1cbTsXLfPtdhV15kDkayImOkA9TDclBBXRyI_l6Gy4QKdGNR4uj7pAH48P7_lzvHl9esnvN3FPmAyx1qbS1JCE61QrRY2hTGeKUzAiSUQqM1FSonFZglKlnAYjUyEpp5QzwVO2QDe_f_uh-xrBh6K1voKmUQ660RecEI6zDE_g9REcyxZ00Q-2VcO--CuGfQP62mO1</recordid><startdate>20010907</startdate><enddate>20010907</enddate><creator>Tan, S M</creator><creator>Walters, S E</creator><creator>Mathew, E C</creator><creator>Robinson, M K</creator><creator>Drbal, K</creator><creator>Shaw, J M</creator><creator>Law, S K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010907</creationdate><title>Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit</title><author>Tan, S M ; Walters, S E ; Mathew, E C ; Robinson, M K ; Drbal, K ; Shaw, J M ; Law, S K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p139t-ddfcd2f147d5daa2ff23d6a72ef84485968b21d0bbeaab9d0bf95892722738753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>CD18 Antigens - genetics</topic><topic>CD18 Antigens - immunology</topic><topic>CD4 Antigens - genetics</topic><topic>CD4 Antigens - metabolism</topic><topic>COS Cells</topic><topic>Cysteine</topic><topic>Epitopes - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Repetitive Sequences, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, S M</creatorcontrib><creatorcontrib>Walters, S E</creatorcontrib><creatorcontrib>Mathew, E C</creatorcontrib><creatorcontrib>Robinson, M K</creatorcontrib><creatorcontrib>Drbal, K</creatorcontrib><creatorcontrib>Shaw, J M</creatorcontrib><creatorcontrib>Law, S K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, S M</au><au>Walters, S E</au><au>Mathew, E C</au><au>Robinson, M K</au><au>Drbal, K</au><au>Shaw, J M</au><au>Law, S K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2001-09-07</date><risdate>2001</risdate><volume>505</volume><issue>1</issue><spage>27</spage><epage>30</epage><pages>27-30</pages><issn>0014-5793</issn><abstract>The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues.</abstract><cop>England</cop><pmid>11557036</pmid><doi>10.1016/S0014-5793(01)02778-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2001-09, Vol.505 (1), p.27-30 |
| issn | 0014-5793 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71170660 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal CD18 Antigens - genetics CD18 Antigens - immunology CD4 Antigens - genetics CD4 Antigens - metabolism COS Cells Cysteine Epitopes - chemistry Molecular Sequence Data Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Repetitive Sequences, Amino Acid |
| title | Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T07%3A20%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Defining%20the%20repeating%20elements%20in%20the%20cysteine-rich%20region%20(CRR)%20of%20the%20CD18%20integrin%20beta%202%20subunit&rft.jtitle=FEBS%20letters&rft.au=Tan,%20S%20M&rft.date=2001-09-07&rft.volume=505&rft.issue=1&rft.spage=27&rft.epage=30&rft.pages=27-30&rft.issn=0014-5793&rft_id=info:doi/10.1016/S0014-5793(01)02778-8&rft_dat=%3Cproquest_pubme%3E71170660%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71170660&rft_id=info:pmid/11557036&rfr_iscdi=true |