Alterations in the Spinal Cord T Cell Repertoire During Relapsing Experimental Autoimmune Encephalomyelitis
The CNS T cell repertoire was analyzed by RT-PCR, spectratyping, and nucleotide sequencing of the amplified products at different times following adoptive transfer of a CD4+, Th1, VB2+ encephalitogenic SJL/J proteolipid protein peptide 139-151-specific T cell clone. The third complementarity-determi...
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| Veröffentlicht in: | The Journal of immunology (1950) 2000-06, Vol.164 (12), p.6662-6668 |
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| container_title | The Journal of immunology (1950) |
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| creator | Fritz, Robert B Wang, Xudong Zhao, Ming-Lang |
| description | The CNS T cell repertoire was analyzed by RT-PCR, spectratyping, and nucleotide sequencing of the amplified products at different times following adoptive transfer of a CD4+, Th1, VB2+ encephalitogenic SJL/J proteolipid protein peptide 139-151-specific T cell clone. The third complementarity-determining region of TCR B chains in the spinal cord was used as an indicator of T cell heterogeneity. Spectratypic analysis revealed that a single peak corresponding to the third complementarity-determining region of the initiating T cell clone predominated during the acute phase. During recovery and relapse the complexity of the spectratype increased. DNA sequence analysis revealed that the donor clone predominated at the acute phase. By the first relapse the donor clone, although represented most frequently, was a minority of the total. Spectratypic analysis of the same samples for several other VB families revealed their presence during acute disease or relapses but, with the exception of VB17, their absence during the recovery stage. |
| doi_str_mv | 10.4049/jimmunol.164.12.6662 |
| format | Article |
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The third complementarity-determining region of TCR B chains in the spinal cord was used as an indicator of T cell heterogeneity. Spectratypic analysis revealed that a single peak corresponding to the third complementarity-determining region of the initiating T cell clone predominated during the acute phase. During recovery and relapse the complexity of the spectratype increased. DNA sequence analysis revealed that the donor clone predominated at the acute phase. By the first relapse the donor clone, although represented most frequently, was a minority of the total. Spectratypic analysis of the same samples for several other VB families revealed their presence during acute disease or relapses but, with the exception of VB17, their absence during the recovery stage.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.164.12.6662</identifier><identifier>PMID: 10843727</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Amino Acid Sequence ; Animals ; Clone Cells - transplantation ; DNA, Complementary - analysis ; Encephalomyelitis, Autoimmune, Experimental - immunology ; Encephalomyelitis, Autoimmune, Experimental - metabolism ; Encephalomyelitis, Autoimmune, Experimental - pathology ; Female ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; Receptors, Antigen, T-Cell, alpha-beta - analysis ; Receptors, Antigen, T-Cell, alpha-beta - genetics ; Recurrence ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Spinal Cord - immunology ; Spinal Cord - metabolism ; Spinal Cord - pathology ; Spleen - cytology ; Spleen - immunology ; Spleen - metabolism ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - metabolism ; T-Lymphocyte Subsets - transplantation ; VB2 protein</subject><ispartof>The Journal of immunology (1950), 2000-06, Vol.164 (12), p.6662-6668</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-a0474ccac575a0fb99ebd39b814211e88486b59151c516a77bd86c6f8964c3213</citedby><cites>FETCH-LOGICAL-c413t-a0474ccac575a0fb99ebd39b814211e88486b59151c516a77bd86c6f8964c3213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10843727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fritz, Robert B</creatorcontrib><creatorcontrib>Wang, Xudong</creatorcontrib><creatorcontrib>Zhao, Ming-Lang</creatorcontrib><title>Alterations in the Spinal Cord T Cell Repertoire During Relapsing Experimental Autoimmune Encephalomyelitis</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>The CNS T cell repertoire was analyzed by RT-PCR, spectratyping, and nucleotide sequencing of the amplified products at different times following adoptive transfer of a CD4+, Th1, VB2+ encephalitogenic SJL/J proteolipid protein peptide 139-151-specific T cell clone. The third complementarity-determining region of TCR B chains in the spinal cord was used as an indicator of T cell heterogeneity. Spectratypic analysis revealed that a single peak corresponding to the third complementarity-determining region of the initiating T cell clone predominated during the acute phase. During recovery and relapse the complexity of the spectratype increased. DNA sequence analysis revealed that the donor clone predominated at the acute phase. By the first relapse the donor clone, although represented most frequently, was a minority of the total. Spectratypic analysis of the same samples for several other VB families revealed their presence during acute disease or relapses but, with the exception of VB17, their absence during the recovery stage.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Clone Cells - transplantation</subject><subject>DNA, Complementary - analysis</subject><subject>Encephalomyelitis, Autoimmune, Experimental - immunology</subject><subject>Encephalomyelitis, Autoimmune, Experimental - metabolism</subject><subject>Encephalomyelitis, Autoimmune, Experimental - pathology</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Sequence Data</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - analysis</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Recurrence</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Spinal Cord - immunology</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord - pathology</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Spleen - metabolism</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - metabolism</subject><subject>T-Lymphocyte Subsets - transplantation</subject><subject>VB2 protein</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P4zAQhi3ECrqFf4CQT4hLuh7HsZNjVcqyEtJKfJwtx51Sg_OBnajw79elIHHb01jj5301My8hZ8Bmgonq17NrmrHt_AykmAGfSSn5AZlAUbBMSiYPyYQxzjNQUh2TnzE-M8Yk4-KIHAMrRa64mpCXuR8wmMF1baSupcMG6X3vWuPpogsr-kAX6D29wx7D0LmA9GoMrn1KHW_6uHst39Kfa7Adkmg-Jmo3GNJla7HfGN817-jd4OIJ-bE2PuLpZ52Sx-vlw-Imu_37-89ifptZAfmQGSaUsNbYQhWGreuqwnqVV3UJggNgWYpS1kUFBdgCpFGqXpXSynVZSWFzDvmUXOx9-9C9jhgH3bho0xqmxW6MWgEoxnL5XxBUIYT6AMUetKGLMeBa92ljE941ML1LQ3-loVMaGrjepZFk55_-Y93g6ptof_4EXO6BjXvabNN1dWyM9wkHvd1uv3v9A-Cvlss</recordid><startdate>20000615</startdate><enddate>20000615</enddate><creator>Fritz, Robert B</creator><creator>Wang, Xudong</creator><creator>Zhao, Ming-Lang</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000615</creationdate><title>Alterations in the Spinal Cord T Cell Repertoire During Relapsing Experimental Autoimmune Encephalomyelitis</title><author>Fritz, Robert B ; Wang, Xudong ; Zhao, Ming-Lang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-a0474ccac575a0fb99ebd39b814211e88486b59151c516a77bd86c6f8964c3213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Clone Cells - transplantation</topic><topic>DNA, Complementary - analysis</topic><topic>Encephalomyelitis, Autoimmune, Experimental - immunology</topic><topic>Encephalomyelitis, Autoimmune, Experimental - metabolism</topic><topic>Encephalomyelitis, Autoimmune, Experimental - pathology</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Sequence Data</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - analysis</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - genetics</topic><topic>Recurrence</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Spinal Cord - immunology</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord - pathology</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Spleen - metabolism</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - metabolism</topic><topic>T-Lymphocyte Subsets - transplantation</topic><topic>VB2 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fritz, Robert B</creatorcontrib><creatorcontrib>Wang, Xudong</creatorcontrib><creatorcontrib>Zhao, Ming-Lang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fritz, Robert B</au><au>Wang, Xudong</au><au>Zhao, Ming-Lang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations in the Spinal Cord T Cell Repertoire During Relapsing Experimental Autoimmune Encephalomyelitis</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2000-06-15</date><risdate>2000</risdate><volume>164</volume><issue>12</issue><spage>6662</spage><epage>6668</epage><pages>6662-6668</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>The CNS T cell repertoire was analyzed by RT-PCR, spectratyping, and nucleotide sequencing of the amplified products at different times following adoptive transfer of a CD4+, Th1, VB2+ encephalitogenic SJL/J proteolipid protein peptide 139-151-specific T cell clone. The third complementarity-determining region of TCR B chains in the spinal cord was used as an indicator of T cell heterogeneity. Spectratypic analysis revealed that a single peak corresponding to the third complementarity-determining region of the initiating T cell clone predominated during the acute phase. During recovery and relapse the complexity of the spectratype increased. DNA sequence analysis revealed that the donor clone predominated at the acute phase. By the first relapse the donor clone, although represented most frequently, was a minority of the total. Spectratypic analysis of the same samples for several other VB families revealed their presence during acute disease or relapses but, with the exception of VB17, their absence during the recovery stage.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>10843727</pmid><doi>10.4049/jimmunol.164.12.6662</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Clone Cells - transplantation DNA, Complementary - analysis Encephalomyelitis, Autoimmune, Experimental - immunology Encephalomyelitis, Autoimmune, Experimental - metabolism Encephalomyelitis, Autoimmune, Experimental - pathology Female Mice Mice, Inbred Strains Molecular Sequence Data Receptors, Antigen, T-Cell, alpha-beta - analysis Receptors, Antigen, T-Cell, alpha-beta - genetics Recurrence Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Spinal Cord - immunology Spinal Cord - metabolism Spinal Cord - pathology Spleen - cytology Spleen - immunology Spleen - metabolism T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism T-Lymphocyte Subsets - transplantation VB2 protein |
| title | Alterations in the Spinal Cord T Cell Repertoire During Relapsing Experimental Autoimmune Encephalomyelitis |
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