Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins
The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hepha...
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| Veröffentlicht in: | American journal of physiology: Gastrointestinal and liver physiology 2001-10, Vol.281 (4), p.G931-G939 |
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| container_issue | 4 |
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| container_title | American journal of physiology: Gastrointestinal and liver physiology |
| container_volume | 281 |
| creator | Frazer, D M Vulpe, C D McKie, A T Wilkins, S J Trinder, D Cleghorn, G J Anderson, G J |
| description | The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined. |
| doi_str_mv | 10.1152/ajpgi.2001.281.4.g931 |
| format | Article |
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The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. 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The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cation Transport Proteins</subject><subject>Cloning, Molecular</subject><subject>Digestive System - metabolism</subject><subject>Hemochromatosis Protein</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>HLA Antigens - genetics</subject><subject>HLA Antigens - metabolism</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Iron - metabolism</subject><subject>Iron-Binding Proteins</subject><subject>Male</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Transferrin - genetics</subject><subject>Receptors, Transferrin - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Tissue Distribution</subject><issn>0193-1857</issn><issn>1522-1547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAQhi0EoqXwE0Ce2BJ8TuwkbKiCglSJBWbLSe3EVWoH25Xg3-OWSkw3vB939yB0CyQHYPRBbqfe5JQQyGkNeZn3TQFnaJ40mgErq3M0J9AUGdSsmqGrELaEEEYBLtEsNbCKQTFHw3J01tgeS7vBvQzRO2OjCtFYOWL1PXkVgnEWO429jHhQ0yCP8iP2apQxaWEwE44Ouzgoj41P7uilDZPzEU_eRWVsuEYXWo5B3ZzmAn2-PH8sX7P1--pt-bTOuoKwmJUKKG-h6FqoW054paWuSUOb9FdTdS3XNddUc9CVprIhegNN19KqKxQozVixQPd_vWnx1z5dKnYmdGocpVVuH0QFwBvgPBnZn7HzLgSvtJi82Un_I4CIA2JxRCwOiEVCLEqxSohT7u60YN_u1OY_dWJa_AJMY3u_</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Frazer, D M</creator><creator>Vulpe, C D</creator><creator>McKie, A T</creator><creator>Wilkins, S J</creator><creator>Trinder, D</creator><creator>Cleghorn, G J</creator><creator>Anderson, G J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins</title><author>Frazer, D M ; Vulpe, C D ; McKie, A T ; Wilkins, S J ; Trinder, D ; Cleghorn, G J ; Anderson, G J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-4e126b13cb18b6067faf8092915297cb6f86f2f61f7f2a90fd19cb27c3e1ef553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cation Transport Proteins</topic><topic>Cloning, Molecular</topic><topic>Digestive System - metabolism</topic><topic>Hemochromatosis Protein</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>HLA Antigens - genetics</topic><topic>HLA Antigens - metabolism</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Iron - metabolism</topic><topic>Iron-Binding Proteins</topic><topic>Male</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Transferrin - genetics</topic><topic>Receptors, Transferrin - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frazer, D M</creatorcontrib><creatorcontrib>Vulpe, C D</creatorcontrib><creatorcontrib>McKie, A T</creatorcontrib><creatorcontrib>Wilkins, S J</creatorcontrib><creatorcontrib>Trinder, D</creatorcontrib><creatorcontrib>Cleghorn, G J</creatorcontrib><creatorcontrib>Anderson, G J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frazer, D M</au><au>Vulpe, C D</au><au>McKie, A T</au><au>Wilkins, S J</au><au>Trinder, D</au><au>Cleghorn, G J</au><au>Anderson, G J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins</atitle><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle><addtitle>Am J Physiol Gastrointest Liver Physiol</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>281</volume><issue>4</issue><spage>G931</spage><epage>G939</epage><pages>G931-G939</pages><issn>0193-1857</issn><eissn>1522-1547</eissn><abstract>The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined.</abstract><cop>United States</cop><pmid>11557513</pmid><doi>10.1152/ajpgi.2001.281.4.g931</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Carrier Proteins - genetics Carrier Proteins - metabolism Cation Transport Proteins Cloning, Molecular Digestive System - metabolism Hemochromatosis Protein Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class I - metabolism HLA Antigens - genetics HLA Antigens - metabolism Humans Immunoblotting Immunohistochemistry Iron - metabolism Iron-Binding Proteins Male Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - metabolism Mice Molecular Sequence Data Rats Rats, Sprague-Dawley Receptors, Transferrin - genetics Receptors, Transferrin - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Tissue Distribution |
| title | Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins |
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