Volumetric and spectroscopic characterizations of the native and acid-induced denatured states of staphylococcal nuclease

We have characterized the acid-induced denaturation of staphylococcal nuclease (SNase) at different urea concentrations by a combination of ultrasonic velocimetry, high precision densimetry, and CD spectroscopy. Our CD spectroscopic results suggest that, at low salt and acidic pH, the protein is unf...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of molecular biology 2000-06, Vol.299 (3), p.827-842
Hauptverfasser:	Filfil, R, Chalikian, T V
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids - metabolism Acids - pharmacology Circular Dichroism Dose-Response Relationship, Drug Hydrogen - metabolism Hydrogen-Ion Concentration Kinetics Micrococcal Nuclease - chemistry Micrococcal Nuclease - metabolism Protein Conformation - drug effects Protein Denaturation - drug effects Protein Folding Solutions Spectrophotometry, Ultraviolet Staphylococcus Thermodynamics Ultrasonics urea Urea - pharmacology Water - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!