Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma
The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3...
Gespeichert in:
| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2000-02, Vol.54 (2-3), p.131-135 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 135 |
|---|---|
| container_issue | 2-3 |
| container_start_page | 131 |
| container_title | Journal of photochemistry and photobiology. B, Biology |
| container_volume | 54 |
| creator | Matsuoka, T Moriyama, N Kida, A Okuda, K Suzuki, T Kotsuki, H |
| description | The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin-200 kD protein complex is localized are labelled by anti-IP3 R antibody. When the anti-IP3 R antibody or antisense oligonucleotide for IP3 receptor is introduced into the living cells of Blepharisma, both the photosensitivity of the cells and content of blepharismin-200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP3 receptor-like protein. |
| doi_str_mv | 10.1016/S1011-1344(00)00007-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71151847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71151847</sourcerecordid><originalsourceid>FETCH-LOGICAL-c305t-6e4ec16ba11ef469c116ca7844518991731a3dd3d20b474f884986b4307ec9a23</originalsourceid><addsrcrecordid>eNpNkE1PwzAMhnMAsTH4CaCcEEgU4iVt2iNMfEyaBBJwjtI0XYPapiTtYRI_nnSbJnKILft97eRB6ALIHRBI7j_CDRFQxq4JuSHh8Cg-QtNDeYJOvf8eG3HCT9AESEqTmNEp-l02zdBaVenGKFlj2cp6443HtsQSd5XtrdNKdyHgztlemxYP3rTroOxNtHyn-NAfK7ktNjho-krjoTVK1_VQS4etW8vW-OYWP9a6q6QLuTxDx6WsvT7fxxn6en76XLxGq7eX5eJhFSlK4j5KNNMKklwC6JIlmQJIlOQpYzGkWQacgqRFQYs5yRlnZZqyLE1yRgnXKpNzOkNXu7nhBz-D9r1ojB-fJlttBy84QJjEeBDGO6Fy1nunS9E500i3EUDEiFpsUYuRqSBEbFGLOPgu9wuGvNHFP9eOM_0D8YJ9YA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71151847</pqid></control><display><type>article</type><title>Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Matsuoka, T ; Moriyama, N ; Kida, A ; Okuda, K ; Suzuki, T ; Kotsuki, H</creator><creatorcontrib>Matsuoka, T ; Moriyama, N ; Kida, A ; Okuda, K ; Suzuki, T ; Kotsuki, H</creatorcontrib><description>The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin-200 kD protein complex is localized are labelled by anti-IP3 R antibody. When the anti-IP3 R antibody or antisense oligonucleotide for IP3 receptor is introduced into the living cells of Blepharisma, both the photosensitivity of the cells and content of blepharismin-200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP3 receptor-like protein.</description><identifier>ISSN: 1011-1344</identifier><identifier>DOI: 10.1016/S1011-1344(00)00007-5</identifier><identifier>PMID: 10836543</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Amino Acid Sequence ; Animals ; Calcium Channels - immunology ; Ciliophora - metabolism ; Cross Reactions ; Fluorescent Antibody Technique, Indirect ; Immunoblotting - methods ; Inositol 1,4,5-Trisphosphate - metabolism ; Inositol 1,4,5-Trisphosphate Receptors ; Molecular Sequence Data ; Perylene - analogs & derivatives ; Perylene - analysis ; Perylene - immunology ; Photoreceptor Cells, Invertebrate - immunology ; Pigments, Biological - analysis ; Pigments, Biological - immunology ; Rats ; Receptors, Cytoplasmic and Nuclear - immunology</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2000-02, Vol.54 (2-3), p.131-135</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-6e4ec16ba11ef469c116ca7844518991731a3dd3d20b474f884986b4307ec9a23</citedby><cites>FETCH-LOGICAL-c305t-6e4ec16ba11ef469c116ca7844518991731a3dd3d20b474f884986b4307ec9a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10836543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuoka, T</creatorcontrib><creatorcontrib>Moriyama, N</creatorcontrib><creatorcontrib>Kida, A</creatorcontrib><creatorcontrib>Okuda, K</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><creatorcontrib>Kotsuki, H</creatorcontrib><title>Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin-200 kD protein complex is localized are labelled by anti-IP3 R antibody. When the anti-IP3 R antibody or antisense oligonucleotide for IP3 receptor is introduced into the living cells of Blepharisma, both the photosensitivity of the cells and content of blepharismin-200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP3 receptor-like protein.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Calcium Channels - immunology</subject><subject>Ciliophora - metabolism</subject><subject>Cross Reactions</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Immunoblotting - methods</subject><subject>Inositol 1,4,5-Trisphosphate - metabolism</subject><subject>Inositol 1,4,5-Trisphosphate Receptors</subject><subject>Molecular Sequence Data</subject><subject>Perylene - analogs & derivatives</subject><subject>Perylene - analysis</subject><subject>Perylene - immunology</subject><subject>Photoreceptor Cells, Invertebrate - immunology</subject><subject>Pigments, Biological - analysis</subject><subject>Pigments, Biological - immunology</subject><subject>Rats</subject><subject>Receptors, Cytoplasmic and Nuclear - immunology</subject><issn>1011-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1PwzAMhnMAsTH4CaCcEEgU4iVt2iNMfEyaBBJwjtI0XYPapiTtYRI_nnSbJnKILft97eRB6ALIHRBI7j_CDRFQxq4JuSHh8Cg-QtNDeYJOvf8eG3HCT9AESEqTmNEp-l02zdBaVenGKFlj2cp6443HtsQSd5XtrdNKdyHgztlemxYP3rTroOxNtHyn-NAfK7ktNjho-krjoTVK1_VQS4etW8vW-OYWP9a6q6QLuTxDx6WsvT7fxxn6en76XLxGq7eX5eJhFSlK4j5KNNMKklwC6JIlmQJIlOQpYzGkWQacgqRFQYs5yRlnZZqyLE1yRgnXKpNzOkNXu7nhBz-D9r1ojB-fJlttBy84QJjEeBDGO6Fy1nunS9E500i3EUDEiFpsUYuRqSBEbFGLOPgu9wuGvNHFP9eOM_0D8YJ9YA</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Matsuoka, T</creator><creator>Moriyama, N</creator><creator>Kida, A</creator><creator>Okuda, K</creator><creator>Suzuki, T</creator><creator>Kotsuki, H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000201</creationdate><title>Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma</title><author>Matsuoka, T ; Moriyama, N ; Kida, A ; Okuda, K ; Suzuki, T ; Kotsuki, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-6e4ec16ba11ef469c116ca7844518991731a3dd3d20b474f884986b4307ec9a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Calcium Channels - immunology</topic><topic>Ciliophora - metabolism</topic><topic>Cross Reactions</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Immunoblotting - methods</topic><topic>Inositol 1,4,5-Trisphosphate - metabolism</topic><topic>Inositol 1,4,5-Trisphosphate Receptors</topic><topic>Molecular Sequence Data</topic><topic>Perylene - analogs & derivatives</topic><topic>Perylene - analysis</topic><topic>Perylene - immunology</topic><topic>Photoreceptor Cells, Invertebrate - immunology</topic><topic>Pigments, Biological - analysis</topic><topic>Pigments, Biological - immunology</topic><topic>Rats</topic><topic>Receptors, Cytoplasmic and Nuclear - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuoka, T</creatorcontrib><creatorcontrib>Moriyama, N</creatorcontrib><creatorcontrib>Kida, A</creatorcontrib><creatorcontrib>Okuda, K</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><creatorcontrib>Kotsuki, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuoka, T</au><au>Moriyama, N</au><au>Kida, A</au><au>Okuda, K</au><au>Suzuki, T</au><au>Kotsuki, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>54</volume><issue>2-3</issue><spage>131</spage><epage>135</epage><pages>131-135</pages><issn>1011-1344</issn><abstract>The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin-200 kD protein complex is localized are labelled by anti-IP3 R antibody. When the anti-IP3 R antibody or antisense oligonucleotide for IP3 receptor is introduced into the living cells of Blepharisma, both the photosensitivity of the cells and content of blepharismin-200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP3 receptor-like protein.</abstract><cop>Switzerland</cop><pmid>10836543</pmid><doi>10.1016/S1011-1344(00)00007-5</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1011-1344 |
| ispartof | Journal of photochemistry and photobiology. B, Biology, 2000-02, Vol.54 (2-3), p.131-135 |
| issn | 1011-1344 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71151847 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Calcium Channels - immunology Ciliophora - metabolism Cross Reactions Fluorescent Antibody Technique, Indirect Immunoblotting - methods Inositol 1,4,5-Trisphosphate - metabolism Inositol 1,4,5-Trisphosphate Receptors Molecular Sequence Data Perylene - analogs & derivatives Perylene - analysis Perylene - immunology Photoreceptor Cells, Invertebrate - immunology Pigments, Biological - analysis Pigments, Biological - immunology Rats Receptors, Cytoplasmic and Nuclear - immunology |
| title | Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T21%3A40%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunochemical%20analysis%20of%20a%20photoreceptor%20protein%20using%20anti-IP3%20receptor%20antibody%20in%20the%20unicellular%20organism,%20Blepharisma&rft.jtitle=Journal%20of%20photochemistry%20and%20photobiology.%20B,%20Biology&rft.au=Matsuoka,%20T&rft.date=2000-02-01&rft.volume=54&rft.issue=2-3&rft.spage=131&rft.epage=135&rft.pages=131-135&rft.issn=1011-1344&rft_id=info:doi/10.1016/S1011-1344(00)00007-5&rft_dat=%3Cproquest_cross%3E71151847%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71151847&rft_id=info:pmid/10836543&rfr_iscdi=true |