Protein Dynamics Enhance Electronic Coupling in Electron Transfer Complexes

Electron-transferring flavoproteins (ETFs) from human and Paracoccus denitrificans have been analyzed by small angle x-ray scattering, showing that neither molecule exists in a rigid conformation in solution. Both ETFs sample a range of conformations corresponding to a large rotation of domain II wi...

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Veröffentlicht in:The Journal of biological chemistry 2001-09, Vol.276 (36), p.34142-34147
Hauptverfasser: Chohan, Kamaldeep K., Jones, Matthew, Grossmann, J. Günter, Frerman, Frank E., Scrutton, Nigel S., Sutcliffe, Michael J.
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container_end_page 34147
container_issue 36
container_start_page 34142
container_title The Journal of biological chemistry
container_volume 276
creator Chohan, Kamaldeep K.
Jones, Matthew
Grossmann, J. Günter
Frerman, Frank E.
Scrutton, Nigel S.
Sutcliffe, Michael J.
description Electron-transferring flavoproteins (ETFs) from human and Paracoccus denitrificans have been analyzed by small angle x-ray scattering, showing that neither molecule exists in a rigid conformation in solution. Both ETFs sample a range of conformations corresponding to a large rotation of domain II with respect to domains I and III. A model of the human ETF·medium chain acyl-CoA dehydrogenase complex, consistent with x-ray scattering data, indicates that optimal electron transfer requires domain II of ETF to rotate by ∼30 to 50° toward domain I relative to its position in the x-ray structure. Domain motion establishes a new “robust engineering principle” for electron transfer complexes, tolerating multiple configurations of the complex while retaining efficient electron transfer.
doi_str_mv 10.1074/jbc.M101341200
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Electron Transport
flavoproteins
Flavoproteins - chemistry
Humans
Oxidation-Reduction
Oxidoreductases, N-Demethylating - chemistry
Paracoccus denitrificans
Paracoccus denitrificans - chemistry
Protein Binding
Protein Conformation
Protein Structure, Tertiary
Scattering, Radiation
X-Rays
title Protein Dynamics Enhance Electronic Coupling in Electron Transfer Complexes
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