Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells

Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly important for the rapid production of recombinant proteins. We improved a process for transient calcium phosphate‐based transfection of HEK293‐EBNA cells in a 1–3 L bioreactor volume. Cells were adapted...

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Veröffentlicht in:	Biotechnology and bioengineering 2001-10, Vol.75 (2), p.197-203
Hauptverfasser:	Meissner, Petra, Pick, Horst, Kulangara, Alexandra, Chatellard, Philippe, Friedrich, Kirstin, Wurm, Florian M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Bioreactors Biotechnology Blotting, Western calcium phosphate Cell Line Culture Media DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Epstein-Barr Virus Nuclear Antigens - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes, Reporter Genetic engineering Genetic Markers Genetic technics Genetic Vectors Green Fluorescent Proteins HEK293-EBNA Humans Immunoglobulin G - genetics Luminescent Proteins Methods. Procedures. Technologies Microscopy, Confocal Modification of gene expression level Plasmids Receptors, Serotonin - genetics Receptors, Serotonin, 5-HT3 Recombinant Proteins - biosynthesis Recombinant Proteins - genetics suspension Time Factors Transfection - methods transient transfection
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