An ELISA-based assay for detergent-solubilized cellular beta 1,4-galactosyltransferase activity. Use of a polyacrylamide derivative with GlcNAc-beta side chains as a solid phase acceptor substrate

In our previous paper [Oubihi et al. (1998) Anal. Biochem., 257, 169-175], we have shown that a polyacrylamide-derived synthetic glycopolymer with GlcNAcbeta side chains, termed PAP(GlcNAcbeta), is useful as a solid phase acceptor substrate for the ELISA-based analyses of soluble beta1,4(-)galactosy...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-04, Vol.64 (4), p.785-792
Hauptverfasser:	Oubihi, M, Oshima, K, Aoki, N, Kobayashi, K, Kitajima, K, Matsuda, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins Animals Cell Differentiation Cell Line COS Cells Detergents Enzyme-Linked Immunosorbent Assay - methods Mice N-Acetyllactosamine Synthase - analysis N-Acetyllactosamine Synthase - genetics Octoxynol Polymers Polystyrenes Sodium Dodecyl Sulfate Solubility Substrate Specificity Tissue Extracts
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