Features of the Replicon of Plasmid pAM10.6 of Pseudomonas fluorescens
We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonas strains but was maintained in Escherichia coli only after the p15A origin of replication was inserted. Deletion analysis suggests that the pAM10.6 origin...
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| Veröffentlicht in: | Plasmid 2001-07, Vol.46 (1), p.25-36 |
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| creator | Peters, Maire Jõgi, Eerik Suitso, Indrek Punnisk, Tiina Nurk, Allan |
| description | We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonas strains but was maintained in Escherichia coli only after the p15A origin of replication was inserted. Deletion analysis suggests that the pAM10.6 origin of replication is located in a 0.5-kb region that includes inverted and direct repeats upstream of the repA gene. RepA (204 aa) has a clear homology to plasmid replication proteins of some other gram-negative bacteria. The pas (plasmid addiction system) (genes encoded in the region of 480-bp) stabilizes plasmid maintenance in P. putida cells under nonselective conditions for at least 200 generations. A 3.75-kb PstI fragment of pAM10.6 joined to a Kmr gene was shown to be a minimal plasmid unit maintained in P. putida as a monomer. Further deletions of this 3.75-kb fragment caused a drive to form stable head-to-tail dimeric plasmids in P. putida. |
| doi_str_mv | 10.1006/plas.2001.1524 |
| format | Article |
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Deletion analysis suggests that the pAM10.6 origin of replication is located in a 0.5-kb region that includes inverted and direct repeats upstream of the repA gene. RepA (204 aa) has a clear homology to plasmid replication proteins of some other gram-negative bacteria. The pas (plasmid addiction system) (genes encoded in the region of 480-bp) stabilizes plasmid maintenance in P. putida cells under nonselective conditions for at least 200 generations. A 3.75-kb PstI fragment of pAM10.6 joined to a Kmr gene was shown to be a minimal plasmid unit maintained in P. putida as a monomer. Further deletions of this 3.75-kb fragment caused a drive to form stable head-to-tail dimeric plasmids in P. putida.</description><identifier>ISSN: 0147-619X</identifier><identifier>EISSN: 1095-9890</identifier><identifier>DOI: 10.1006/plas.2001.1524</identifier><identifier>PMID: 11535033</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Dimerization ; DNA, Bacterial ; Escherichia coli ; Gene Dosage ; Kmr gene ; Molecular Sequence Data ; pas gene ; plasmid addiction system ; plasmid dimerization ; plasmid replicon ; Plasmids ; Pseudomonas fluorescens ; Pseudomonas fluorescens - genetics ; Pseudomonas putida ; Pseudomonas specific ; RepA ; repA gene ; Replicon ; Sequence Analysis, DNA</subject><ispartof>Plasmid, 2001-07, Vol.46 (1), p.25-36</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-27bc08c3b0a1c89b8f723579b4c392033137564e45b10e7ca3a7291f7f88f8013</citedby><cites>FETCH-LOGICAL-c371t-27bc08c3b0a1c89b8f723579b4c392033137564e45b10e7ca3a7291f7f88f8013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/plas.2001.1524$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11535033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peters, Maire</creatorcontrib><creatorcontrib>Jõgi, Eerik</creatorcontrib><creatorcontrib>Suitso, Indrek</creatorcontrib><creatorcontrib>Punnisk, Tiina</creatorcontrib><creatorcontrib>Nurk, Allan</creatorcontrib><title>Features of the Replicon of Plasmid pAM10.6 of Pseudomonas fluorescens</title><title>Plasmid</title><addtitle>Plasmid</addtitle><description>We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonas strains but was maintained in Escherichia coli only after the p15A origin of replication was inserted. Deletion analysis suggests that the pAM10.6 origin of replication is located in a 0.5-kb region that includes inverted and direct repeats upstream of the repA gene. RepA (204 aa) has a clear homology to plasmid replication proteins of some other gram-negative bacteria. The pas (plasmid addiction system) (genes encoded in the region of 480-bp) stabilizes plasmid maintenance in P. putida cells under nonselective conditions for at least 200 generations. A 3.75-kb PstI fragment of pAM10.6 joined to a Kmr gene was shown to be a minimal plasmid unit maintained in P. putida as a monomer. Further deletions of this 3.75-kb fragment caused a drive to form stable head-to-tail dimeric plasmids in P. putida.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Dimerization</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli</subject><subject>Gene Dosage</subject><subject>Kmr gene</subject><subject>Molecular Sequence Data</subject><subject>pas gene</subject><subject>plasmid addiction system</subject><subject>plasmid dimerization</subject><subject>plasmid replicon</subject><subject>Plasmids</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas putida</subject><subject>Pseudomonas specific</subject><subject>RepA</subject><subject>repA gene</subject><subject>Replicon</subject><subject>Sequence Analysis, DNA</subject><issn>0147-619X</issn><issn>1095-9890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFLxDAQRoMo7rp69Sg9eeuaadomOS6Lq8KKIgreQppOsNI2tWkF_72pu-BJPIUJ7_tmeIScA10CpflVV2u_TCiFJWRJekDmQGUWSyHpIZlTSHmcg3ydkRPv32kIJJAfkxlAxjLK2JxsNqiHsUcfORsNbxg9YVdXxrXT_BjKm6qMutV92Jf_fHkcS9e4VvvI1qMLSYOtPyVHVtcez_bvgrxsrp_Xt_H24eZuvdrGhnEY4oQXhgrDCqrBCFkIyxOWcVmkhskkHASMZ3mKaVYARW400zyRYLkVwgoKbEEud71d7z5G9INqqnBAXesW3egVB0hZlrB_QeASALgI4HIHmt5536NVXV81uv9SQNWkWE2K1aRYTYpD4GLfPBYNlr_43mkAxA7AIOKzwl55U2FrsKx6NIMqXfVX9zfgh4gZ</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Peters, Maire</creator><creator>Jõgi, Eerik</creator><creator>Suitso, Indrek</creator><creator>Punnisk, Tiina</creator><creator>Nurk, Allan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Features of the Replicon of Plasmid pAM10.6 of Pseudomonas fluorescens</title><author>Peters, Maire ; Jõgi, Eerik ; Suitso, Indrek ; Punnisk, Tiina ; Nurk, Allan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-27bc08c3b0a1c89b8f723579b4c392033137564e45b10e7ca3a7291f7f88f8013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Dimerization</topic><topic>DNA, Bacterial</topic><topic>Escherichia coli</topic><topic>Gene Dosage</topic><topic>Kmr gene</topic><topic>Molecular Sequence Data</topic><topic>pas gene</topic><topic>plasmid addiction system</topic><topic>plasmid dimerization</topic><topic>plasmid replicon</topic><topic>Plasmids</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas putida</topic><topic>Pseudomonas specific</topic><topic>RepA</topic><topic>repA gene</topic><topic>Replicon</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peters, Maire</creatorcontrib><creatorcontrib>Jõgi, Eerik</creatorcontrib><creatorcontrib>Suitso, Indrek</creatorcontrib><creatorcontrib>Punnisk, Tiina</creatorcontrib><creatorcontrib>Nurk, Allan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plasmid</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peters, Maire</au><au>Jõgi, Eerik</au><au>Suitso, Indrek</au><au>Punnisk, Tiina</au><au>Nurk, Allan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Features of the Replicon of Plasmid pAM10.6 of Pseudomonas fluorescens</atitle><jtitle>Plasmid</jtitle><addtitle>Plasmid</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>46</volume><issue>1</issue><spage>25</spage><epage>36</epage><pages>25-36</pages><issn>0147-619X</issn><eissn>1095-9890</eissn><abstract>We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonas strains but was maintained in Escherichia coli only after the p15A origin of replication was inserted. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Base Sequence Dimerization DNA, Bacterial Escherichia coli Gene Dosage Kmr gene Molecular Sequence Data pas gene plasmid addiction system plasmid dimerization plasmid replicon Plasmids Pseudomonas fluorescens Pseudomonas fluorescens - genetics Pseudomonas putida Pseudomonas specific RepA repA gene Replicon Sequence Analysis, DNA |
| title | Features of the Replicon of Plasmid pAM10.6 of Pseudomonas fluorescens |
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