Effect of peroxynitrite on glutaredoxin
Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can...
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| Veröffentlicht in: | Human & experimental toxicology 2001-07, Vol.20 (7), p.373-376 |
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| creator | Aykaç-Toker, G Bulgurcuogélu, S Koçak-Toker, N |
| description | Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO- on glutaredoxin, rat liver cytosolic fractions were incubated with 0–250 MONOO -. Thioltransferase activity was found to be decreased as ONOO- concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely. |
| doi_str_mv | 10.1191/096032701680350578 |
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As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO- on glutaredoxin, rat liver cytosolic fractions were incubated with 0–250 MONOO -. Thioltransferase activity was found to be decreased as ONOO- concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely.</description><identifier>ISSN: 0960-3271</identifier><identifier>EISSN: 1477-0903</identifier><identifier>DOI: 10.1191/096032701680350578</identifier><identifier>PMID: 11530836</identifier><language>eng</language><publisher>Thousand Oaks, CA: SAGE Publications</publisher><subject>Acids ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cell Culture Techniques ; dehydroascorbate reductase ; Enzymes ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; glutaredoxin ; Glutaredoxins ; Homeostasis ; Liver - drug effects ; Male ; Nitric oxide ; Oxidative stress ; Oxidoreductases - drug effects ; Oxidoreductases - metabolism ; Peroxynitrous Acid - pharmacology ; Potassium ; Proteins - drug effects ; Proteins - metabolism ; Rats ; Rats, Wistar ; thioltransferase ; Toxicology ; Transferases ; Variance analysis</subject><ispartof>Human & experimental toxicology, 2001-07, Vol.20 (7), p.373-376</ispartof><rights>2001 INIST-CNRS</rights><rights>2001 Arnold</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-9325cb42f7160405fb9584dd97c63bc1be318ad01041a4bc7df07691db894a133</citedby><cites>FETCH-LOGICAL-c426t-9325cb42f7160405fb9584dd97c63bc1be318ad01041a4bc7df07691db894a133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1191/096032701680350578$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1191/096032701680350578$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,778,782,21953,27840,27911,27912,44932,45320</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1191/096032701680350578?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1089960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11530836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aykaç-Toker, G</creatorcontrib><creatorcontrib>Bulgurcuogélu, S</creatorcontrib><creatorcontrib>Koçak-Toker, N</creatorcontrib><title>Effect of peroxynitrite on glutaredoxin</title><title>Human & experimental toxicology</title><addtitle>Hum Exp Toxicol</addtitle><description>Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO- on glutaredoxin, rat liver cytosolic fractions were incubated with 0–250 MONOO -. Thioltransferase activity was found to be decreased as ONOO- concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely.</description><subject>Acids</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques</subject><subject>dehydroascorbate reductase</subject><subject>Enzymes</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glutaredoxin</subject><subject>Glutaredoxins</subject><subject>Homeostasis</subject><subject>Liver - drug effects</subject><subject>Male</subject><subject>Nitric oxide</subject><subject>Oxidative stress</subject><subject>Oxidoreductases - drug effects</subject><subject>Oxidoreductases - metabolism</subject><subject>Peroxynitrous Acid - pharmacology</subject><subject>Potassium</subject><subject>Proteins - drug effects</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>thioltransferase</subject><subject>Toxicology</subject><subject>Transferases</subject><subject>Variance analysis</subject><issn>0960-3271</issn><issn>1477-0903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkE1Lw0AQhhdRbK3-AQ8SRPQUO5Pd7MdRSv2Aghc9h81mt6SkSd1NoP33tjZQUdDTXJ73mZmXkEuEe0SFY1AcaCIAuQSaQirkERkiEyIGBfSYDHdAvCVwQM5CWAAAVymekgFiSkFSPiR3U-esaaPGRSvrm_WmLltftjZq6mheda32tmjWZX1OTpyugr3o54i8P07fJs_x7PXpZfIwiw1LeBsrmqQmZ4kTyIFB6nKVSlYUShhOc4O5pSh1AQgMNcuNKBwIrrDIpWIaKR2R27135ZuPzoY2W5bB2KrStW26kAlERhngvyDKBBnyHXj9A1w0na-3T2RJApKn8GVL9pDxTQjeumzly6X2mwwh25Wd_S57G7rqzV2-tMUh0re7BW56QAejK-d1bcrwTS3Vzjoi4z0W9Nwervtj8yeSb5CG</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Aykaç-Toker, G</creator><creator>Bulgurcuogélu, S</creator><creator>Koçak-Toker, N</creator><general>SAGE Publications</general><general>Arnold</general><general>Sage Publications Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7ST</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Effect of peroxynitrite on glutaredoxin</title><author>Aykaç-Toker, G ; Bulgurcuogélu, S ; Koçak-Toker, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-9325cb42f7160405fb9584dd97c63bc1be318ad01041a4bc7df07691db894a133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acids</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Culture Techniques</topic><topic>dehydroascorbate reductase</topic><topic>Enzymes</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glutaredoxin</topic><topic>Glutaredoxins</topic><topic>Homeostasis</topic><topic>Liver - drug effects</topic><topic>Male</topic><topic>Nitric oxide</topic><topic>Oxidative stress</topic><topic>Oxidoreductases - drug effects</topic><topic>Oxidoreductases - metabolism</topic><topic>Peroxynitrous Acid - pharmacology</topic><topic>Potassium</topic><topic>Proteins - drug effects</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>thioltransferase</topic><topic>Toxicology</topic><topic>Transferases</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aykaç-Toker, G</creatorcontrib><creatorcontrib>Bulgurcuogélu, S</creatorcontrib><creatorcontrib>Koçak-Toker, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human & experimental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Aykaç-Toker, G</au><au>Bulgurcuogélu, S</au><au>Koçak-Toker, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of peroxynitrite on glutaredoxin</atitle><jtitle>Human & experimental toxicology</jtitle><addtitle>Hum Exp Toxicol</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>20</volume><issue>7</issue><spage>373</spage><epage>376</epage><pages>373-376</pages><issn>0960-3271</issn><eissn>1477-0903</eissn><abstract>Glutaredoxin is an important enzyme in thiol homeostasis. 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| subjects | Acids Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Culture Techniques dehydroascorbate reductase Enzymes Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology glutaredoxin Glutaredoxins Homeostasis Liver - drug effects Male Nitric oxide Oxidative stress Oxidoreductases - drug effects Oxidoreductases - metabolism Peroxynitrous Acid - pharmacology Potassium Proteins - drug effects Proteins - metabolism Rats Rats, Wistar thioltransferase Toxicology Transferases Variance analysis |
| title | Effect of peroxynitrite on glutaredoxin |
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