Horizontal gene transfer and host specificity of beta‐haemolytic streptococci: the role of a putative composite transposon containing scpB and lmb
Beta‐haemolytic streptococci are important human and animal pathogens: their genetic traits that are associated with the ability to infect human hosts remain, however, unclear. The surface protein, Lmb, mediates the adherence of Streptococcus agalactiae to human laminin. For further analysis of the...
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| Veröffentlicht in: | Molecular microbiology 2001-08, Vol.41 (4), p.925-935 |
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| creator | Franken, C. Haase, G. Brandt, C. Weber‐Heynemann, J. Martin, S. Lämmler, C. Podbielski, A. Lütticken, R. Spellerberg, B. |
| description | Beta‐haemolytic streptococci are important human and animal pathogens: their genetic traits that are associated with the ability to infect human hosts remain, however, unclear. The surface protein, Lmb, mediates the adherence of Streptococcus agalactiae to human laminin. For further analysis of the corresponding gene, the adjacent genomic regions were sequenced. Lmb is localized on a putative composite transposon of 16 kb and is flanked by two copies of a novel insertion sequence element (ISSag2). It harbours the genes scpB and lmb, which are 98% identical with the respective genes of Streptococcus pyogenes. Analysis of the distribution of these genes and ISSag2 among 131 streptococcal strains revealed that all of the human isolates, but only 20% (12 of 61) of the animal isolates, contained scpB and lmb or their homologues. To investigate if the putative transposon can be mobilized, an erythromycin resistance marker was incorporated into the lmb gene of S. agalactiae. Screening for mutant strains with a regained susceptibility for erythromycin identified strains with a deletion of scpB, lmb, and one copy of ISSag2. We hypothesize that a horizontal gene transfer caused the exchange of scpB and lmb and that the ability of S. pyogenes, S. agalactiae and group C and G streptococcal strains to colonize or infect human hosts is dependent on their presence. |
| doi_str_mv | 10.1046/j.1365-2958.2001.02563.x |
| format | Article |
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The surface protein, Lmb, mediates the adherence of Streptococcus agalactiae to human laminin. For further analysis of the corresponding gene, the adjacent genomic regions were sequenced. Lmb is localized on a putative composite transposon of 16 kb and is flanked by two copies of a novel insertion sequence element (ISSag2). It harbours the genes scpB and lmb, which are 98% identical with the respective genes of Streptococcus pyogenes. Analysis of the distribution of these genes and ISSag2 among 131 streptococcal strains revealed that all of the human isolates, but only 20% (12 of 61) of the animal isolates, contained scpB and lmb or their homologues. To investigate if the putative transposon can be mobilized, an erythromycin resistance marker was incorporated into the lmb gene of S. agalactiae. Screening for mutant strains with a regained susceptibility for erythromycin identified strains with a deletion of scpB, lmb, and one copy of ISSag2. We hypothesize that a horizontal gene transfer caused the exchange of scpB and lmb and that the ability of S. pyogenes, S. agalactiae and group C and G streptococcal strains to colonize or infect human hosts is dependent on their presence.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2001.02563.x</identifier><identifier>PMID: 11532154</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science, Ltd</publisher><subject>Adhesins, Bacterial - genetics ; Adhesins, Bacterial - physiology ; Animals ; Bacterial Adhesion ; Base Sequence ; Blotting, Southern ; DNA Transposable Elements - genetics ; DNA, Ribosomal - genetics ; Endopeptidases - genetics ; Endopeptidases - physiology ; Evolution, Molecular ; Gene Dosage ; Gene Transfer, Horizontal - genetics ; Genes, Bacterial - genetics ; Humans ; insertion sequence ISSag2 ; laminin ; Lmb protein ; Molecular Sequence Data ; Phylogeny ; Repetitive Sequences, Nucleic Acid ; RNA, Ribosomal, 16S - genetics ; scpB gene ; Sequence Alignment ; Streptococcal Infections - microbiology ; Streptococcus agalactiae ; Streptococcus agalactiae - genetics ; Streptococcus agalactiae - physiology ; Streptococcus pyogenes</subject><ispartof>Molecular microbiology, 2001-08, Vol.41 (4), p.925-935</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. 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The surface protein, Lmb, mediates the adherence of Streptococcus agalactiae to human laminin. For further analysis of the corresponding gene, the adjacent genomic regions were sequenced. Lmb is localized on a putative composite transposon of 16 kb and is flanked by two copies of a novel insertion sequence element (ISSag2). It harbours the genes scpB and lmb, which are 98% identical with the respective genes of Streptococcus pyogenes. Analysis of the distribution of these genes and ISSag2 among 131 streptococcal strains revealed that all of the human isolates, but only 20% (12 of 61) of the animal isolates, contained scpB and lmb or their homologues. To investigate if the putative transposon can be mobilized, an erythromycin resistance marker was incorporated into the lmb gene of S. agalactiae. Screening for mutant strains with a regained susceptibility for erythromycin identified strains with a deletion of scpB, lmb, and one copy of ISSag2. We hypothesize that a horizontal gene transfer caused the exchange of scpB and lmb and that the ability of S. pyogenes, S. agalactiae and group C and G streptococcal strains to colonize or infect human hosts is dependent on their presence.</description><subject>Adhesins, Bacterial - genetics</subject><subject>Adhesins, Bacterial - physiology</subject><subject>Animals</subject><subject>Bacterial Adhesion</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Endopeptidases - genetics</subject><subject>Endopeptidases - physiology</subject><subject>Evolution, Molecular</subject><subject>Gene Dosage</subject><subject>Gene Transfer, Horizontal - genetics</subject><subject>Genes, Bacterial - genetics</subject><subject>Humans</subject><subject>insertion sequence ISSag2</subject><subject>laminin</subject><subject>Lmb protein</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>scpB gene</subject><subject>Sequence Alignment</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus agalactiae</subject><subject>Streptococcus agalactiae - genetics</subject><subject>Streptococcus agalactiae - physiology</subject><subject>Streptococcus pyogenes</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAURS0EokPhF5DFgl1SO45jG4kFVIVWasUGJHaW43nueJTEwXZohxWfwIIv5EuadEYgsYGVn-zz7pV1EMKUlJTUzcm2pKzhRaW4LCtCaEkq3rDy9gFa_X54iFZEcVIwWX0-Qk9S2s4gIw17jI4o5ayivF6hn-ch-m9hyKbD1zAAztEMyUHEZljjTUgZpxGsd976vMPB4Ray-fX9x8ZAH7pd9hanHGHMwQZr_SucN4Bj6GBhDR6nbLL_CtiGfgzJ50PDPIdhvpyL_eCHa5zs-Pa-s-vbp-iRM12CZ4fzGH16d_bx9Ly4_PD-4vTNZWFrwVghmsa1jonaVKCE4LWRjEhS1aKFSloqmLWCKKocqJY7pmTbcGegFqDqNbTsGL3c544xfJkgZd37ZKHrzABhSlpQWldSqn-CVFLFqVrAF3-B2zDFYf6EpqrhiyYxQ3IP2RhSiuD0GH1v4k5Tohe_eqsXjXrRqBe_-t6vvp1Xnx_yp7aH9Z_Fg9AZeL0HbnwHu_8O1ldXF8vE7gBCCbdh</recordid><startdate>200108</startdate><enddate>200108</enddate><creator>Franken, C.</creator><creator>Haase, G.</creator><creator>Brandt, C.</creator><creator>Weber‐Heynemann, J.</creator><creator>Martin, S.</creator><creator>Lämmler, C.</creator><creator>Podbielski, A.</creator><creator>Lütticken, R.</creator><creator>Spellerberg, B.</creator><general>Blackwell Science, Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200108</creationdate><title>Horizontal gene transfer and host specificity of beta‐haemolytic streptococci: the role of a putative composite transposon containing scpB and lmb</title><author>Franken, C. ; Haase, G. ; Brandt, C. ; Weber‐Heynemann, J. ; Martin, S. ; Lämmler, C. ; Podbielski, A. ; Lütticken, R. ; Spellerberg, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4733-766fbf374a2e97754a83080247be28c173cc70919fe9b5f398b65fae47e94deb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>Adhesins, Bacterial - physiology</topic><topic>Animals</topic><topic>Bacterial Adhesion</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Endopeptidases - genetics</topic><topic>Endopeptidases - physiology</topic><topic>Evolution, Molecular</topic><topic>Gene Dosage</topic><topic>Gene Transfer, Horizontal - genetics</topic><topic>Genes, Bacterial - genetics</topic><topic>Humans</topic><topic>insertion sequence ISSag2</topic><topic>laminin</topic><topic>Lmb protein</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>scpB gene</topic><topic>Sequence Alignment</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus agalactiae</topic><topic>Streptococcus agalactiae - genetics</topic><topic>Streptococcus agalactiae - physiology</topic><topic>Streptococcus pyogenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Franken, C.</creatorcontrib><creatorcontrib>Haase, G.</creatorcontrib><creatorcontrib>Brandt, C.</creatorcontrib><creatorcontrib>Weber‐Heynemann, J.</creatorcontrib><creatorcontrib>Martin, S.</creatorcontrib><creatorcontrib>Lämmler, C.</creatorcontrib><creatorcontrib>Podbielski, A.</creatorcontrib><creatorcontrib>Lütticken, R.</creatorcontrib><creatorcontrib>Spellerberg, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Franken, C.</au><au>Haase, G.</au><au>Brandt, C.</au><au>Weber‐Heynemann, J.</au><au>Martin, S.</au><au>Lämmler, C.</au><au>Podbielski, A.</au><au>Lütticken, R.</au><au>Spellerberg, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Horizontal gene transfer and host specificity of beta‐haemolytic streptococci: the role of a putative composite transposon containing scpB and lmb</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2001-08</date><risdate>2001</risdate><volume>41</volume><issue>4</issue><spage>925</spage><epage>935</epage><pages>925-935</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Beta‐haemolytic streptococci are important human and animal pathogens: their genetic traits that are associated with the ability to infect human hosts remain, however, unclear. The surface protein, Lmb, mediates the adherence of Streptococcus agalactiae to human laminin. For further analysis of the corresponding gene, the adjacent genomic regions were sequenced. Lmb is localized on a putative composite transposon of 16 kb and is flanked by two copies of a novel insertion sequence element (ISSag2). It harbours the genes scpB and lmb, which are 98% identical with the respective genes of Streptococcus pyogenes. Analysis of the distribution of these genes and ISSag2 among 131 streptococcal strains revealed that all of the human isolates, but only 20% (12 of 61) of the animal isolates, contained scpB and lmb or their homologues. To investigate if the putative transposon can be mobilized, an erythromycin resistance marker was incorporated into the lmb gene of S. agalactiae. Screening for mutant strains with a regained susceptibility for erythromycin identified strains with a deletion of scpB, lmb, and one copy of ISSag2. We hypothesize that a horizontal gene transfer caused the exchange of scpB and lmb and that the ability of S. pyogenes, S. agalactiae and group C and G streptococcal strains to colonize or infect human hosts is dependent on their presence.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science, Ltd</pub><pmid>11532154</pmid><doi>10.1046/j.1365-2958.2001.02563.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesins, Bacterial - genetics Adhesins, Bacterial - physiology Animals Bacterial Adhesion Base Sequence Blotting, Southern DNA Transposable Elements - genetics DNA, Ribosomal - genetics Endopeptidases - genetics Endopeptidases - physiology Evolution, Molecular Gene Dosage Gene Transfer, Horizontal - genetics Genes, Bacterial - genetics Humans insertion sequence ISSag2 laminin Lmb protein Molecular Sequence Data Phylogeny Repetitive Sequences, Nucleic Acid RNA, Ribosomal, 16S - genetics scpB gene Sequence Alignment Streptococcal Infections - microbiology Streptococcus agalactiae Streptococcus agalactiae - genetics Streptococcus agalactiae - physiology Streptococcus pyogenes |
| title | Horizontal gene transfer and host specificity of beta‐haemolytic streptococci: the role of a putative composite transposon containing scpB and lmb |
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